In vitro protein synthesis and α amylase activity in F cells from hepatopancreas ofPalaemon serratus (Crustacea; Decapoda)

In crustaceans, all the steps in the assimilation of food take place in the hepatopancreas. To facilitate the study of this organ, a method for the dissociation of cell types was developed. The hepatopancreas of the prawnPalaemon serratus was mechanically dissociated and the cells separated by Percoll density-gradient centrifugation. The E and R cells had similar densities of around 1.05 g/ml. The F cells were separated into two distinct fractions with densities of 1.075 and 1.082 g/ml. The B cells sedimented at a density of 1.12 g/ml. The ratio between the two populations of F cells was found to vary during the intermolt cycle while B cells disappeared after the molt. When the density gradient fractions were incubated with³H-leucine, incorporation was highest in the F cell fractions. Measurements of -amylase activity, indicated that the two populations of F cells may be derived from the same cell type.     

产物分离固相化细胞反应器的设计和数学模型

设计了一种以挥发性产物和非挥发性底物为特征的四相反应器——分离器。在此反应器中,生物催化剂被固定在固体柱填充物上并与含有底物的液相接触,汽相流过柱子而将挥发性产物蒸发迸入汽相。反应器由同方向流的浓缩器和反方向流的分离器两个基本汽——液流部分组成。并建立了吸附的单层细胞稳态群体数学模型和反应器平衡状态模型。     

Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells

Summary Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG₁ antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascitec fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody.     

Selective protection of cells against X-irradiation. Isoproterenol protects only those cells that possesβ-adrenoreceptors

Summary In mixed culture of Chinese hamster fibroblatst, clone 431, and transformed murine L fibroblasts, clone B-82, isoproterenol was found to protect only 431 cells against ionizing radiation. It was shown that 431 cells, in contrast to B-82 cells, possess-adrenoreceptors, and the radioprotective effect of isoproterenol can be realized only if this agent interacts with-adrenoreceptors coupled with the cAMP system. Since malignization often causes the disappearance of-adrenergic and other hormone receptors, the combined culturing and irradiation of the cells studied can be regarded as a model of the growth of malignant cells (B-82) among normal tissue cells (431 cells) under conditions of radiation therapy. A possibility of selective protection against radiation damage of normal tissue cells, with retention of the former radiosensitivity of tumor cells, is discussed.     

联合实体边界检测的命名实体识别方法

针对传统命名实体识别方法无法有效利用实体边界信息的问题,提出一种联合实体边界检测的命名实体识别方法,即将实体边界检测作为辅助任务,增强模型对实体边界的判断能力,进而提升模型对实体的识别效果。首先,利用Bert预训练语言模型对原始文本进行特征嵌入获取词向量,并引入自注意力机制增强词对上下文信息的利用;其次,在命名实体识别任务的基础上,添加实体边界检测辅助任务,增强模型对实体边界的识别能力;再次,对比联合实体边界检测的命名实体识别方法与基线方法的有效性,并对测试结果进行消融实验;最后,进行样例分析,分析损失权重β对实体边界检测的影响。实验结果表明,在英文社交媒体数据集Twitter-2015上,联合实体边界检测的命名实体识别方法相较于基线模型取得了更高的精准率、召回率和F1值,其中F1值达到了73.57%;并且,边界检测辅助任务提升了基线方法的检测效果。所提方法能有效利用实体边界信息,从而获得更好的实体识别效果,促进了人机交互系统的发展,对自然语言处理下游任务有重要意义。     

数据驱动下交通异常行为的双层识别模型

为实现对车辆异常行为的准确识别,提高车辆行驶的安全性,提出了一种基于支持向量机(support vector machine, SVM)、监督学习与长短期记忆(long short term memory, LSTM)深度学习的交通异常驾驶行为双层识别模型。首先,对车辆轨迹数据筛除和滤波,构建异常行为数据集;其次,从异常行为轨迹特征中提取出特定异常行为的特征标签,并人为标定在训练集中;再次,构建SVM模型对训练集进行粗识别,基于SVM的二分法原理,从测试集中筛选出异常行为;最后,通过LSTM时间序列模型构建具体种类的异常行为模型,并通过深度学习的方法,从异常行为数据中细分为蛇形驾驶、急速变向、侧滑、大半径转弯、快速U型转弯、急刹车等具体的异常驾驶行为。本次实验选用下一代仿真(next generation simulation, NGSIM)数据中US-101高速公路和peachtree城市道路的数据集的轨迹数据验证SVM和LSTM双层识别模型的性能,包括均方根误差、识别准确率等。结果表明,构建的双层识别模型在第一层有98%的识别准确率,第二层有超过80%的识别准确率,可以较为准确地识...     

利用早停可微架构搜索的三维点云模型分类

针对现有三维点云分类网络采用人工设计费时费力的问题,提出早停可微架构搜索(early-stopping differentiable architecture search,ES-DARTS)算法。利用从人工设计网络架构中提取到的先验知识,预定义一个包含高效候选操作的搜索空间,可快速搜索出适用于三维模型分类任务的高性能网络模型;通过追踪网络搜索阶段各候选操作的权重变化,找出跳跃连接操作在双重优化过程中发挥不公平竞争作用的临界点并在此处停止搜索,以保证各候选操作之间的稳定性,解决DARTS算法搜索过程中易出现性能崩溃的问题。提出的算法在ModelNet40数据集上达到了93.2%的识别准确率,比当前人工设计的主流网络具有更高的识别准确率。     

白蜡虫(Ericerus pela Chavannes)雌虫神经分泌系统的形态及组织学研究

应用Ewen’s改良多聚醛品红染色方法,对雌性白蜡虫(Ericerus pela Cha-vannes)成虫神经分泌系统的形态及组织学进行了研究.确定了在前脑中存在有两群属于“A”型神经分泌细胞.愈合腹神经节中亦有分散的“A”型神经分泌细胞.脑神经分泌细胞中的神经分泌粒通过其轴突传送到心侧体中释放.心侧体为神经血器官.虫体在进入成体阶段后到产卵期间,其脑中的神经分泌细胞在形态及染色性上都没有明显差异.     

Bovine microvascular endothelial cells of separate morphology differ in growth and response to the action of interferon-γ

Five cell types recently isolated from the bovine corpus luteum differed in their epithelioid morphology and their cytoskeleton, but shared common criteria of microvascular endothelial cells^1,2. To give strong evidence for the separate entity, the growth rate of the 5 phenotypically different cells was studied. They were seeded at low density on day 0. Most of these cells were treated with 200 to 1000 U recombinant bovine interferon- (IFN-) for 3 days. The untreated remainder served as controls. Cell counts were made for all cultures on days 4, 7, 10 and 13. morphology: 13 d after treatment with IFN- senescent cells as well as intact cells occurred in cultures of cell types 1 to 4. Cultures of cell type 5 were apparently unchanged and resembled their untreated counterparts. Desminpositive cells in cultures of cell type 2 developed cell processes. Growth rate: In the absence of IFN-, the growth rate was high for cell types 3 and 4, moderate for cell type 1, and low for cell types 2 and 5. The presence of IFN- caused anti-proliferative effects. These were higher for cell types 3 and 4 than for cell types 1 and 2. IFN- could be cytotoxic on cell type 3. In contrast, the cytokine tended to support the cell growth of cell type 5. These findings substantiate the postulate that endothelial cells exhibiting separate morphology in culture also function differently.     

细胞内游离钙的荧光测定

由于细胞内游离钙浓度是重要的生理指标之一,所以重点在测定细胞内游离钙的技术要求、影响测定的重要因素、钙的荧光试剂以及新进展四个方面叙述。实验证明Ｗａｚｏ－１试剂对细胞无毒性,与钙反应迅速、灵敏及选择性高,细胞内游离钙的荧光测定快速准确。但是,荧光试剂的合成路线较长,还需要改进。