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131.
L. De Petrocellis V. Di Marzo G. Cimino 《Cellular and molecular life sciences : CMLS》1993,49(1):57-64
The participation of protein kinase C (PKC) in the regeneration of tentacles ofHydra vulgaris was studied. Regeneration was induced by 1,2-sn-dioctanoyl-glycerol (diC8) and the novel diterpenoidic diacylglycerol verrucosin B (VB), a potent PKC activator extracted from marine sources. VB substantially increasedHydra average tentacle number (ATN) at concentrations 10,000 times lower than those needed for diC8 to exert an analogous effect. When both synthetic and natural VB analogues were tested, the structure/activity relationship found inHydra tentacle regeneration was identical to that known for DAG-induced activation of PKC in vitro. VB-induced increase of ATN was strongly counteracted by the PKC inhibitors sphingosine and A3, but was not synergic with a tenfold increase of extracellular Ca2+ concentration or with an increase of intracellular Ca2+ concentration obtained either with the ionophore A23187 or with thapsigargin. This suggested the involvement of a non-Ca2+-dependent PKC in VB-triggeredHydra tentacle regeneration. The involvement of phospholipase A2 (PLA2) activation inHydra regenerative processes was studied using the novel site-specific inhibitor of the enzyme, oleyloxyethylphosphorylcholine (OOPC), which brought about a striking inhibition of ATN in the low molar range. This effect was reversed by arachidonic acid (AA), while an enhancement of ATN was also observed with an inhibitor of AA uptake from membrane phospholipids, thus suggesting that PLA2-catalysed liberation of AA is involved inHydra tentacle regeneration. OOPC also blocked verrucosin B-induced PKC-mediated enhancement of ATN, thus suggesting that this effect is also mediated by PLA2 activation. ATN was increased also by compound 48/80, a direct activator of pertussis toxin-sensitive GTP-binding proteins, and this effect was counteracted by pertussis toxin pretreatment. None of the known AA cascade inhibitors exhibited an effect on ATN comparable to that exerted by OOPC, but, surprisingly, the cycloxygenase inhibitor indomethacin strongly enhanced ATN, thus suggesting that prostanoids might effect a negative control onHydra regenerative processes. This represents the first attempt so far reported to study the implication of more than one biochemical pathway as a signalling event in the hydroid regenerative processes. 相似文献
132.
几个高蛋白冬小麦品种在冀东地区的生态适应性 总被引:1,自引:0,他引:1
以全国不同生态类型区的8个高蛋白冬小麦品种为材料,试验研究了其在冀东地区的生态适应性,并进行了综合评价。结果表明,超优66和京农97-86两个品种单株次生根、单株干物重、分蘖成穗率、籽粒产量以及蛋白质产量均较高,成熟期适宜,抗倒性较好,适宜在冀东地区大面积推广种植。 相似文献
133.
变色酸2R共振光散射光谱法测定蛋白质 总被引:7,自引:1,他引:7
基于人血清白蛋白(HAS)对酸性偶氮染料变色酸2R的共振光散射的增强效应,拟定了一种新的测定HSA的共振光散射法。在pH=4.10的条件下,变色酸2R本身只有极弱的光散射,但它与蛋白质的结合物却有强烈的共振光散射作用,在λex=λcm=420nm处,光散射有最大的散射强度,并且光散射强度与蛋白质HSA的浓度成线性关系,线性范围为0.0-10.0μg/mL,检测限可达0.12μg/ml。该法简便、快速,用于人体血清样品蛋白质的测定并与考马斯亮蓝法比较,结果令人满意。 相似文献
134.
利用培养新生大鼠心肌细胞,检测NO前体L-精氨酸(L-arginine,L-Arg)和NO供体硝普钠(sodium nitroprusside,SNP)对PKC活性的影响,并探讨内、外源性NO在PKC激动剂佛波指(phorbol 12-myristate 13-acetate,PMA)激活PKC中的作用.实验结果表明:培养基中加入L-Arg,PKC活性呈剂量依赖性降低;用L-Arg进行预处理,30 min后加入PMA,PKC活性明显降低,与单纯PMA组相比有显著差异;NOS抑制剂L-NAME本身对基础状态PKC活性无明显影响,但可阻断L-Arg对上述2个效应的影响;培养液中加入NO供体SNP,PKC活性呈剂量依赖性降低;用SNP预处理心肌细胞,5 min后加入PMA,PKC活性与单纯PMA组相比有显著性差异.以上结果表明,内、外源性NO均具有剂量依赖性抑制PKC活性的作用,PKC可能是NO对心肌细胞作用的胞内信号传导通路的关键部位或重要信号分子之一;L-Arg通过NOS先生成NO,NO再对PKC起抑制作用. 相似文献
135.
To observe the regulation of platelet-derived growth factor (PDGF) receptor-βin myocyte stimulated by angiotensin II (AngII) at both integrated and cellular levels and reveal the signal transduction mechanism in cell, two kidneys, one clip (2K1C) renal hypertension were performed by placing a sliver clip around the left renal artery. Blood pressure and the ratio of left ventricular weight to body weight were measured at 4 and 8 weeks after operation. The content of AngII in heart was detected by radioimmunology assay; the protein level of PDGF receptor-βin heart was measured by Western blot analysis. The alteration of PDGF receptor-βstimulated by AngII and several inhibitors was observed on cultured neonatal rat ventricular myocyte (NRVM). The content of AngII in heart of 2K1C renal hypertensive rat at 4 and 8 weeks after operation was increased. Compared with sham group, 4 and 8 weeks after operation, PDGF receptor-βin heart of 2K1C group was upregulated by 100.3% and 127.1% (P < 0.05), respectively. This upregulation could be inhibited by captopril. For cultured myocyte, PDGF receptor-βwas increased by 47.1% after being stimulated by AngII and this upregulation could be inhibited by losartan which was an inhibitor of AT1 receptor. PLC inhibitor (U73122) and MEK inhibitor (PD98059) could partly inhibit PDGF receptor-βupregulation induced by AngII. These results suggested that AngII could upregulate PDGF receptor-βin myocyte by its AT1 receptor and this effect was at least partly dependent on PLC and extracellular signal-regulated kinase (ERK). 相似文献
136.
近红外光谱定量分析小麦粉蛋白质含量 总被引:3,自引:0,他引:3
基于逐步多元线性回归的算法,对小麦粉的近红外光谱进行了蛋白质浓度的定量分析。通过对原始近红外光谱在波长范围1300~1860nm内,采用不同波长间隔的逐步回归,提取了较理想的回归波长,建立了少参量数学模型。对不同未知样本进行了预测,结果表明,所建模型具有一定的稳定性,对未知样品蛋白质浓度的测量偏差较小,其最大偏差不超过1.0。 相似文献
137.
138.
Computer simulations at the atomic level have arrived at a stage where they provide realistic modeling of flexibility in proteins
(and the mobility of their associated solvent) that is important in understanding the nature of molecular motions. This can
now be extended to the molecular and atomic motions that are associated with protein mechanisms. Moreover, the derived data
agree reasonably accurately with experimental measurements of several kinetic and thermodynamic parameters. Fundamental insights
emerge on the roles that this intrinsic flexibility plays in the thermodynamic characteristics of macromolecules in solution;
these equip the investigator to probe the consequences of cognate interactions and ligand binding on entropy and enthalpy.
Thus simulations can now provide a powerful tool for investigating protein mechanisms that complements the existing and the
emerging experimental techniques.
Received 29 May 2005; received after revision 23 August 2005; accepted 21 October 2005 相似文献
139.
Oshima T Sasaki M Kataoka H Miwa H Takeuchi T Joh T 《Cellular and molecular life sciences : CMLS》2007,64(23):3139-3147
Tight junctions (TJs) create a paracellular permeability barrier. Although reactive oxygen species have been implicated as
mediators of inflammation in inflammatory bowel diseases, their influence on the function of colonic epithelial TJs remains
unknown. Oxidative stress-mediated colonic epithelial permeability was significantly attenuated by a p38 mitogen-activated
protein (MAP) kinase inhibitor, SB203580. Although the amount of TJ proteins was not altered, hydrogen peroxide (H2O2) changed the localization of claudin-4 protein from an NP-40 insoluble fraction to a soluble fraction and from an apical
TJ to lateral membrane. The p38 MAP kinase inactivator Wip1 significantly attenuated phosphorylation of p38 MAP kinase, and
oxidative stress mediated permeability. H2O2-induced changes in claudin-4 localization were abolished by SB203580 pretreatment as well as Wip1-expressing adenovirus infection.
This is the first study to demonstrate that exogenous Wip1 functions to protect oxidative stress-mediated colonic mucosal
permeability and that H2O2-induced claudin-4 dislocalization is abolished by Wip1.
Received 14 June 2007; received after revision 8 October 2007; accepted 8 October 2007 相似文献
140.
Delhommeau F Pisani DF James C Casadevall N Constantinescu S Vainchenker W 《Cellular and molecular life sciences : CMLS》2006,63(24):2939-2953
Myeloproliferative disorders (MPDs) are clonal haematopoietic malignancies involving the abnormal proliferation of myeloid
lineages. The World Health Organisation (WHO) classification of haematopoietic malignancies distinguishes MPDs from myelodysplastic/
myeloproliferative disorders and systemic mastocytosis. These malignancies frequently involve constitutive tyrosine kinase
activity, resulting from either oncogenic fusion protein production or from point mutations. Chronic myelogenous leukaemia
is the model used for studies of the consequences of such molecular defects. However, the heterogeneity of the clinical course
of MPDs should be seen in a more rationale conceptual framework, including the many molecular events associated with these
diseases. This review focuses on the various tyrosine kinase-related molecular mechanisms underlying both MPDs and rare diseases
with myeloproliferative features. We pay particular attention to the newly identified JAK2 V617F mutation in polycythaemia
vera, essential thrombocythaemia and idiopathic myelofibrosis and deal with disease heterogeneity and putative additional
molecular mechanisms.
Received 9 June 2006; received after revision 28 July 2006; accepted 11 September 2006 相似文献