稻壳糟蛋白饲料的研究

本文报道利用热带假丝酵母(Candida tropicalis 2.637)以固体糟为底物,在发酵料中加入锌终浓度15ppm、钼20ppm,稀土元素0.1％,尿素2％时,30℃培养48小时后,粗蛋白含量由本底20.93％提高到36.21％。     

蛋白质结构类预测的新方法：基于蛋白质二级结构序列的预测方法

提出了由蛋白质二级结构序列预测蛋白质结构类的新方法，并给出了预测蛋白质结构类的简明预测规则。     

淡水白鲳营养需要量的研究

本文报导用蛋白质梯度试验确定淡水白鲳最适饵料蛋白含量，用生长率为指标求得的结果为２７．２３一３７．４０％；结合蛋白质效率与生长率为指标求得的结果为３１．４２％。在此基础上设计正交试验，用生长比速结合蛋白质效率和饵料系数为评价指标确定每１００ｇ淡水白鲳对蛋白质、糖、脂肪、纤维素和无机盐的日需要量分别为１．５７ｇ、１．８９ｇ、０．３０ｇ、０．１０ｇ和０．２０ｇ。     

植物细胞钙靶蛋白的研究进展

简述了植物细胞的钙离子调控过程，对钙调蛋白、钙调磷酸酶B类蛋白和钙离子依赖型蛋白质激酶3类钙靶蛋白的最新研究进展进行了综述。     

MAP kinase specifically mediates the ABA-induced H<Subscript>2</Subscript>O<Subscript>2</Subscript> generation in guard cells of<Emphasis Type="Italic">Vicia faba</Emphasis> L.

The plant hormone abscisic acid (ABA) is involved in regulating adverse physiological processes, including stomatal closure, seed development and germination, and mediating many environmental stress responses, such as drought, salinity and extreme temperatures[1,2]. In re-sponse to various stress stimuli, ABA synthesis is in-creased in plant cells, which triggers a series of physio-logical responses to adapt the stress conditions[1—3]. For example, under water deficit, ABA acts directly on…     

Characterization of a calmodulin binding protein kinase from<Emphasis Type="Italic">Arabidopsis thalian</Emphasis>

A full-length calmodulin binding protein kinase cDNA ,AtCBK1 ,from Arabidopsis has been isolated by screening of an Arabidopsis cDNA library and by 5′-RACE-Northern blot and in situ hybridization indicated that the expression of AtCBK1 was more abundant in the vascular bundles and the meristems than in other tissues,The phylogenetic analyses revenl that AtCBK1 is different from animal CaMKs and it falls into CRK subgroup,indicating that they may come from different ancestors,The result suggests that AtCBK1 encoldes a CaM-binding serine/threonine protein kinase.     

Modulating multidrug resistance through inhibiting of protein kinase C activity by phenothiazines

In the present study, actions of phenothiazines(PTZ) in reversing multidrug resistance(MDR) and inhibiting PKC activity were investigated. It was found that the three PTZs caused 2.49, 36.58 and 75.78 fold reversal of K562/AO2 MDR cells resistant to adriamycin, respectively, while the chemosensitizer verapamil caused 40 fold reversal in the same condition, indicating that PTZ11 is a novel reversal agent of MDR and a potential chemotherapeutic reagent for tumor therapy. PKC activity analysis in the presence of PTZs showd that PTZ6 and PTZ11 inhibited rat brain protein kinase C activity in a manner of dose_dependent. The IC 50 values were (489.77±31.4) and (113±9.64) μmol/L, respectively. PTZ7 had no inhibition on PKC activity. Further study showed that PTZ11 could reduce PMA_mediated activation of PKC in a manner of dose_dependent, suggesting that PTZ11 might compete for the high_affinity phorbol ester binding site within PKC molecule. Recently, an X_ray structure of PMA in complex with PKC Cys2 activator_binding domain was solved. We therefore decided to explore the possible binding model of PTZ11 with PKC molecule using SYBYL 6.02 program. It was shown that the binding site of PTZ11 with PKC molecule partially overlapped with that of PMA, providing for the first time new data for designing PKC inhibitors and MDR reversal drugs.     

T-cell signal transduction and the role of protein kinase C

The T lymphocyte has a vital part to play in maintaining the host response to bacterial and viral infection and also appears to play a key pathological role in autoimmune diseases such as rheumatoid arthritis. In this review, we summarize the signalling pathways which trigger antigen-driven T-cell proliferation and examine the evidence which suggests that protein kinase C (PKC) is fundamental to this process. Finally, we discuss the therapeutic potential that PKC inhibitors may have in the treatment of autoimmune disease. Received 31 March 1998; received after revision 19 May 1998; accepted 19 May 1998     

磺化铝酞菁在微量蛋白分析中的应用

利用蛋白质对磺化铝酞菁的吸附特性，建立了一种测量微量蛋白的方法．观测了多种影响因素，并探讨了其机理，与传统方法相比，本法具有简便快速的特点，测定结果较满意．牛血清白蛋白测定的线性范围为０．６７～８．００ｍｇ／Ｌ，检出限为０．５５ｍｇ／Ｌ，精密度为６．７％