用聚合酶链式反应扩增抗冻肽基因并在双元表达载体中克隆

抗冻肽基因导入植物的第一步合成了５＇－末端分别带有限制性酶ｘｂａＩ和ＫｐｎＩ识别位点的一对引物，以美洲拟鲽抗冻肽的ｃＤＮＡ克隆为模板，用锚定聚合酰酶链反应扩增了抗冻肽基因，并克隆到细菌表达载体ｐＧＥＭ３２ｆ（一）和植物表达盒式载体ｐＧＡ６４３中，以限制性酶切分析和菌落杂交技术证明了转化子质粒ＤＮＡ中的含有抗冻肽基因，又用ＰＣＲ方法扩增了转化子的抗冻肽基因，从而证实了克隆成功。     

果蝇细胞周期蛋白Cyclin D在胚胎发育不同时期的表达

细胞周期蛋白Cyclin D（CycD）是发育中重要的蛋白,作者利用原位杂交法和免疫组化法,分别对野生型的果蝇w1118以及本研究室构建的一个转基因株系cycD-lacZ进行分析,从而建立了在果蝇发育的早期胚胎发育各阶段cycDmRNA的表达模式.结果表明：Stage 5时期,cycDmRNA集中于胚胎表面新生成的细胞处.Stage10,在发育中的神经系统,前中肠和后中肠处可以检测到cycD的mRNA.Stage14,cycD基因于中肠有着强烈的表达.cycDmRNA在胚胎发育中的表达模式的确立,为后期研究CycD在胚胎发育中所起的作用奠定了基础.     

Localization and the possible role of plasminogen activators and inhibitors in early stages of placentation

The distribution of mRNAs of tissue type (t) and urokinase type (u) plasminogen activator (PA) plus their corresponding inhibitors, type-1 (PAI-1) and type-2 (PAI-2) have been studied in the tissues of human first and second trimester placentae by in situ hybridization. The results show that: (ⅰ) All the molecules, tPA, uPA, PAI-1 and PAI-2, were identified in the blood vessels, the majority of extravillous trophoblastic cells of the decidual layer between Rohr’s and Nitabuch’s stria and in the trophoblast cells lining the chorionic plate, basal plate, intercotyledonary septae and cytotrophoblast cells of the chorionic villous tree. (ⅱ) No expression of such probes was observed in the basal and chorionic plate, glandular cells of the decidua, the septal tissues or the villous core mesenchyme. The co-distribution of the molecules observed suggests that the co-ordinated expression of the activators and inhibitors in various cells of the placental tissue may play a role in angiogenesis related to conversion of spiral arteries into utero-placental arteries and establishment of a chorio-decidual blood flow during early stages of placentation.     

考虑行为主体的置换流水车间干扰管理研究

针对置换流水车间计划外新工件到达干扰问题,研究从加工系统参与主体:企业管理者、车间工人和客户三方进行基于前景理论的扰动度量,并建立同时考虑初始成本目标和基于行为运筹扰动目标的干扰管理模型.经分析发现问题复杂度为NP-难,为了弥补单一元启发式算法具有求解性能依赖于初始解的质量以及局部搜索不足等缺陷,从初始解改进和加强局部搜索两个角度,提出一种具有一般通用性的元启发式算法混合策略,分别基于量子计算、粒子群搜索和模拟退火设计了四种具体混合算法.为验证该混合策略的有效性,基于置换流水车间Taillard(Ta)标准测试集设计干扰算例并应用算法进行求解,验证了基于前景理论的扰动度量的优势,通过输出有效前沿多样性和收敛性分析证明了所提算法混合策略求解问题的有效性.     

后殖民主义入侵下的翻译策略

以往的翻译理论研究大多是在语言学的层面上进行,即以语言分析为基础。而后殖民主义翻译理论则摆脱了单一语言研究的束缚,使翻译研究从文本内部走向了社会、文化及政治等领域。运用后殖民主义的相关理论对归化、异化、杂合化翻译策略进行分析,有助于探索如何在抵制文化霸权的同时弘扬我国的优秀传统文化。     

Rapid diagnosis with FISH for chromosomal abnormality of fetal pyelectasia

Fluorescence in situ Hybridization （FISH） was used to investigate whether the chromosome of the fetus prenatally diagnosed as pyelectasis was normal or not. Amniotic fluid was taken from the pregnant woman whose fetus was detected with pyelectasia by prenatal examination. The chromosome of the amniotic fluid cell without culture was examined with FISH. The result shows that compared with the traditional amniotic fluid cell culture, FISH has the advantages of more rapid, higher sensitivity and specificity, and was 10-12 days earlier to complete the diagnosing than the traditional method. The fetuses detected chromosomal abnormality in each groups were induced during the middle and late trimester, while those fetuses with normal chromosome continued pregnancy, the rate of spontaneous disappearance of pyelectasia decreased as the severity of pyelectasia increased. FISH can satisfy the urgent need in the clinical prenatal diagnosis due to its rapidity to determine whether fetus with pyelectasia was accompanied with chromosomal.     

大规模原位杂交筛选爪蟾发育调控基因

应用半自动化的系统表达筛选,研究了2400个爪蟾EST克隆在胚胎发育中的表达模式,从筛选出的624个分化表达克隆中,发现了协同表达组的新成员及调控爪蟾体节形成的新基因.     

芪丹颗粒剂对肺纤维化鼠Ⅰ型前胶原和Ⅲ型前胶原mRNA表达的影响

观察芪丹颗粒对博莱霉素致肺纤维化鼠的Ⅰ型前胶原和Ⅲ型前胶原mRNA表达的影响,探讨其作用机制.用160只SD大鼠按随机区组设计分为对照组(N)、模型组(M)、芪丹颗粒剂1组和2组(Q1、Q2)、氢化可的松1组和2组(H1、H2)共6组.对照组20只气管内灌注和灌胃均用生理盐水.余140只SD大鼠经气管内一次性灌注博莱霉素A5按BLM 5 mg·kg-1体重诱导大鼠肺间质纤维化,随机取40只为模型组.芪丹1组和氢可1组分别从造模后第2天灌注芪丹颗粒剂(3 125 mg/kg)和腹腔注射氢化可的松(25mg/kg),药物干预后第7、14、28天分别处死动物.芪丹2组和氢可2组分别从造模14天后灌注芪丹颗粒剂和腹腔注射氢化可的松(用量同前),2组动物第28、42天分别处死.用苏木素-伊红(HE)评价肺组织病理学变化和原位杂交方法检测各组大鼠肺Ⅰ型和Ⅲ型前胶原mRNA表达.肺组织病理HE染色显示芪丹组肺泡炎及肺纤维化程度均明显轻于模型组和氢可组(P＜0.05).在肺间质纤维化形成中,Ⅰ型和Ⅲ型前胶原mRNA表达呈动态变化,早期肺泡炎以Ⅲ型前胶原mRNA大量增生为主,晚期纤维化期以Ⅰ型前胶原mRNA增生为主.芪丹组Ⅲ型前胶原mRNA的表达在第14天处于最高,至28天仍维持较高的水平,芪丹组Ⅲ型前胶原mRNA的表达高于模型组和氢可组(P＜0.05).Ⅰ型前胶原mRNA的表达在28天时芪丹1组和氢可1组及芪丹2组和氢可2组组间均有显著性差异,但在42天时,芪丹2组与氢化可的松2组其表达无显著差异(P＜0.05).大鼠肺纤维化的早期以Ⅲ型前胶原mRNA的表达为主,晚期纤维化期以Ⅰ型前胶原mRNA的大量表达为主.芪丹颗粒可减轻博莱要素诱导大鼠肺泡炎及肺纤维化的程度,其机制可能通过影响了Ⅰ型和Ⅲ型前胶原mRNA的代谢和表达,从而减慢肺间质纤维化的进程.芪丹颗粒对肺间质纤维化有治疗作用,且优于氢化可的松.     

rhGM-CSF/IL-3融合蛋白中残余菌体DNA的检测

本文利用地高辛标记及检测试剂盒,标记ｐｏｐ２１３６（ＰＭＴＧ３）工程菌染色体ＤＮＡ作为探针,检测基因工程药品重组人粒细胞－巨噬细胞集落刺激因子／白细胞介素－３（ｒｈＧＭ－ＣＳＦ／ＩＬ－３）融合蛋白中残余的菌体ＤＮＡ．结果符合质量标准每剂＜１００ｐｇ．     

Chromosomalin situ hybridization ofCyprinus carpio genomic DNA repetitive sequence CR1

Common carpCyprinus carpio genomic DNA repetitive sequence CR1 has been DIG-labeled and hybridizedin situ against chromosomes of red common carp (Cyprinus carpio L. Xingguo red var. ). It is found that the repetitive sequence CR1 is mainly localized at the centromeric regions of chromosomes of the red common carp. The application of the chromosomalin situ hybridization technique on fish and the relationship between CR1 repetitive sequence distribution and its function have been discussed.