绿色荧光裸鼠部分免疫学指标的测定及分析

摘要: 目的检测绿色荧光蛋白( Green Fluorescence Protein,简称GFP) 转基因裸鼠的部分免疫学指标,分析GFP 转基因裸鼠与其他小鼠在免疫学方面的优缺点,为将来的研究提供基础参考值。方法① 实验选用6 ～ 8 周GFP 转基因裸鼠、BALB/c 裸鼠和BALB/c 小鼠,活体称体质量,剖取脾脏称其质量,之后进行脾淋巴细胞增殖能力的测定。② 取外周血,测定部分血常规指标及免疫球蛋白指标。结果① GFP 转基因裸鼠脾脏质量、脾脏系数以及T、B 淋巴细胞增殖反应与BALB/c 裸鼠相近,但与BALB/c 小鼠差异显著。② GFP 转基因裸鼠、BALB/c 裸鼠两种裸鼠品系外周血白细胞水平、淋巴细胞以比例及免疫球蛋白IgG、IgM 都远低于BALB/c 小鼠,差异显著。结论从脏器系数、白细胞水平,免疫球蛋白水平等角度发现,GFP 转基因裸鼠和BALB/c 裸鼠的免疫系统水平较BALB/c 小鼠相弱,其中GFP 转基因裸鼠的免疫系统水平最为低下。     

黄道婆对手工棉纺织生产发展的贡献

本文根据宋末元初之际,江南地区的社会经济和纺织技术发展状况,以及海南岛黎族人民的植棉和棉纺织技术发展水平,探讨了元代杰出的纺织家黄道婆对上海地区棉纺织业生产发展的贡献。作者认为黄道婆的主要贡献可以概括为三个方面:一.手工棉纺织生产机具上的革新和创造,特别是发明了被后世人们称为“黄道婆纺车”的供纺棉用的脚踏三锭纺车;二.开发了棉织物的新品种,特别是色织棉布和提花棉织物,创造了名扬天下的“鸟泥泾被”;三.推动了以上海县为中心的松江府一带的手工棉纺织生产的商品化过程,在松江府发展为全国最大的手工棉纺织业中心,有“衣被天下”之誉。     

A simulation study on the thermal and wetting behavior of alkane thiol SAM on gold (111) surface

Molecular dynamics simulations have been performed to investigate the structural,thermal and wetting properties of self-assembled monolayer(SAM) of alkane thiol on gold surface.The specific heat capacity of the gold SAM surface was found to linearly increase with the temperature in the range 100–300 K.It was found to drop down at 400 K and this decrease might be attributed to the disorder of the SAM chains.Hydration of gold SAM surface for two different terminal groups,namely methyl(hydrophobic),and hydroxy(hydrophilic) was studied at room temperature.The difference in their wetting behavior and the structure of their interfacial water were examined from the estimation of the z density profile,radial distribution function,hydrogen bonds and orientation of water dipoles in the interfacial region.The present simulation results suggest that the wetting behavior of the gold SAM surface can be modified by altering the terminal functional group of the SAM chains.     

基于广域网的金相显微镜远程监控系统

介绍了针对大型金相显微镜设计的远程监控系统。该系统通过广域网传输监控信号，利用微机和单片机实现对金相显微镜的远程监控。同时，提出了动态适应性编码方法，从而很好地协调了传输速度和传输数据量之间的矛盾，实现了视频数据的实时传输。     

基于核方法的分类型属性数据集模糊聚类算法

针对分类型属性数据的聚类问题．将核方法的思想推广到快速、高效率的模糊c-均值算法,构造了基于核函数的模糊核c-均值聚类算法．该算法通过使用经验核矩阵充分利用了数据间的“相异性”信息,并且避免了模糊k-modes算法中每次迭代均要直接计算类中心的缺点,提高了聚类的精确度和稳定性．同时该算法对模式(类中心)的初始值选择不敏感．时实际的线性可分的和线性不可分的分类型属性数据集的仿真实验证明了该算法的有效性．     

实现“（dE／dt）~（－1）－E”倒数示波图的模拟电路参数选择和单板机装置

对实现“（ｄＥ／ｄｔ）－１－Ｅ”倒数示波图的实验装置进行了进一步深入研究：改进了以往的模拟电路，考察了电参数的影响；研制了一台单板机装置．     

超几何多项式_3F_2(-n,n+a,b;n+c,-n+d;z)零点的渐近分布(英文)

利用经典的分析方法导出了一类超几何多项式3F2(-n,n+a,b;n+c,-n+d;z)零点的渐近分布.     

Protein kinases mediate nitric oxide-induced apoptosis in the insect cell line IPLB-LdFB

The involvement of protein kinases (PKA, PKC and PKB) in nitric oxide (NO)-induced apoptosis with sodium nitroprusside plus N-acetyl-L-cysteine in the IPLB-LdFB cell line from the insect Lymantria dispar was investigated. The presence of protein kinase-like molecules was demonstrated by western blot analysis. The role of the kinases in programmed cell death was analysed in cytofluorimetric experiments by incubating the insect cells with H-89 (a specific inhibitor of PKA), calphostin C (an inhibitor of PKC) or wortmannin (an inhibitor of phosphatidylinositol 3-kinase). The results show that PKA is correlated with the induction and PKC and PKB with the prevention of NO-induced insect cell death. Moreover, NO-induced apoptosis involves the release of cytochrome c. Received 15 March 2002; accepted 25 March 2002     

具不变集的平面三次系统极限环的不存在性

用定性分析的方法,证明了具有两条三次代数曲线解ｙ＾２＝（ａｘ＾３＋ｂｘ）＾２的平面三次系统无级限环,但可以有奇闭轨。