溶氧对黄原胶发酵的影响

黄原胶是野油菜黄单孢菌分泌的胞外多糖,可用淀粉或葡萄糖为碳源发酵生产。本文着重以淀粉为碳源研究不同的溶氧水平对多糖的影响。试验结果表明:溶氧水平高于饱和度的20%对多糖发酵影响不大,低于20%时菌的生长受影响。溶氧水平为饱和度的25%,菌体生长最佳。     

福建丽藻属二新种

本文报导了丽藻属的二新种。分别定名为异分枝丽藻(Nitella heterofurcata和穗枝丽藻(Nitella spicata),前者采自福建邵武下沙乡,后者采自福建尤溪双鲤乡。     

云南木兰科新植物

1 显脉木兰新种 Magnolia phanerophlebia B. L. Chen, sp. nov. Arbor parva glaberrima, c. 3 m alta. 6 cm in diam., cortice incano laevi: ramuli junniores virides in sicco cinerascentes et pruinosi. Folia sempervirentia, lamina rigide coriacea, obovata vel obovato-elliptica'35-43cm longa, 10-17 cm lata, apice acuta vel breviter acuminata, basi anguste cuneata, supra nitidissima     

内蒙古孩儿参属的研究

本文确认了内蒙古境内分布的6种孩儿参属Psendostelaria Pax植物,比1978年出版的《内蒙古植物志》第二卷增加了4种,其中P.japonica(Korsh.)Pax、P.rupetris(Turcz.)Pax、P.maximowicziana(Franch.et Sav.)Pax为內蒙古分布新记录种,而P.helanshanensis W.Z.Di et Y.Ren为新种收载种。本文否定了新种P.termnialis W.Z.Di et Y.Ren它应为P.ropestris(Turez.)Pax的新异名。     

偶氮染料及其还原产物的微生物降解

从印染厂排出水中分离出了两种细菌。其中的邻单胞菌Plesiomonas sp.X_(13)菌株对五种偶氮染料均有脱色作用。脱色率达75％以上;脱色产物中有芳香胺,但X_(13)菌株对苯胺在振荡培养或深层静止培养条件下均无降解能力。另一种,无色细菌Achromobacter sp.A_8菌株对脱色过程中产生的芳香胺则具有降解作用,其降解能力也因培养条件而异。在振荡培养条件下,其降解芳香胺的速率远比静止培养时为快。     

中国盘菌目新属和新种及其系统地位

本文描述了产自中国云南省盘菌目Pezizales新属——假地耳属Otideopsis Liu et Cao gen.nov。和新种——云南假地耳O.Yunnanensis Liu et Cao sp.nov.,并对其在盘菌目中的系统地位作了论述。     

昆嵛山萱草属(Hemerocallis L.)植物的核型及其染色体变异研究

研究了两种来自昆嵛山萱草属（Hemerocallis）植物的染色体数目、核型，结果表明：黄花莱（H．citrina）的体细胞中期染色体核型为2n=2X=22=16m＋4sm＋2T。核型不对称系数为58．9％，染色体相对长度组成为2n=2X=22=6L＋10M1＋6S。染色体总体积为105．39μm^3；对于北萱草（Hesculenta），以上各项则分别为2n=2X=22=20m＋2T、53．8％、2n=2X=22=4L＋2M2＋14M1＋2S和99．43μm^3．同时对染色体的性状进行了巢式方差分析，表明居群内个体间、细胞间染色体的形态和大小具有一定程度的变异。且居群内的变异主要来自于个体间．     

Characterization, development and exploitation of EST-derived microsatellites in Gossypium raimondii Ulbrich

COTTON IS AN IMPORTANT GLOBAL CASH CROP. IN THE RECENTYEARS, MOLECULAR MARKER TECHNOLOGY HAS BEEN WIDELY APPLIED TO SUCH STUDIES ON COTTON AS GENETIC MAP- PING[1―4], VARIETY PURITY DETECTION[5], GENETIC DIVERSITY ANALYSIS[6], MOLECULAR MARKER-ASSISTED BR…     

Cloning, prokaryotic expression,purification and sequence analysis of 20S proteasome subunit gene from T.harzianum

The gene encoding the 20S proteasome subunit（PR29） was cloned from cDNA library of Trichoderma harzianum and expressed in Escherichia coli BL21 （D3） using a pET-28a expression system. The molecular weight of the protein was found to be approximately 29 kDa, as estimated by SDS-PAGE on gels. The target protein was insoluble when induced at 22℃ with 0.4 mmol/L IPTG, while dissoluble if induced at 37℃ with 0.8mmoL/L IPTG. The expressed product was purified through Ni-magnetic beads His Bind. The purity of the fusion protein reached above 80%. The entire eDNA sequence consisted of 1094 bp with 173 and 135 bp in 5＇ and 3＇ untranslated regions respectively. The gene encoding 261 amino acids has no signal peptide sequence. These results could provide a basis for validating the func-tions of PR29. It also provided a preliminary indication for further study of the mechanism and function of proteasome, and more information of proteasome mechanism in T.harzianum could be obtained.