Induction of nicotine in tobacco by herbivory and its relation to glucose oxidase activity in the labial gland of three noctuid caterpillars

Tobacco Nicotiana tabacum L. is a host plant ofHelicoverpa armigera (Huibner), Helicoverpa assulta Guenee and Spodoptera litura (Fabricius) (Lepidoptera, Noctuidae). The difference in leaf nicotine response to the feeding by these three larvae and the mechanical simulation of their feeding was examined by HPLC. Results indicated that nicotine induction was suppressed by H. armigera and H. assulta larvae feeding or by simulated damage treated with their labial glands extracts. The production of nicotine was also suppressed by the glucose oxidase from Aspergillus niger when it was treated on mechanically wounded leaf area. On the contrary, the nicotine production was stimulated by S. litura larva feeding or by simulated damage treated with its labial gland extract. Heat denature can not counteract the stimulation effect of the S. litura labial gland extracts to tobacco nicotine production. The glucose oxidase activity was detected in labial gland extracts of both H. armigera and H. assulta, but the activity in H. armigera was significantly higher than that in H. assulta. No glucose oxidase activity was detected in labial gland extracts of S. litura. It is shown that the glucose oxidase activity in labial glands of caterpillars plays an important role in the nicotine response to herbivory. The glucose oxidase was mainly contained in the labial gland of H. armigera larva, and had the highest activity at pH 7.0. D-Glucose was the optimal substrate of the glucose oxidase. Labial gland glucose oxidase activities varied daily during larval development with high activities found when larvae were actively feeding.     

血涂片中出现有核红细胞的临床意义

目的观察血涂片中出现有核红细胞的病因及临床意义，方法新鲜血片经瑞氏，姬姆萨染色，光学显微镜镜检，结果100例血片中出现有核红细胞的病例分布于12种疾病中，结论：观察血片中有核红细胞的形态，对某些疾病的诊断有重要的临床意义。     

Java多线程同步机制平台相关性浅析

Java多线程具有很大的平台相关性，导致了其同步机制的平台相关性。通过试验讨论了Java同步机制在不同平台下的成功实现和实现效率，并分析了这种平台相关性的原因和影响因素，对处理多线程同步机制的Java程序的跨平台性编写和执行具有借鉴意义。     

片姜黄质量标准再评价研究

为对片姜黄的质量标准进行再评价研究,并完善其质量标准,建立了吉马酮含量测定的方法,在原标准的基础上增加吉马酮的含量测定.结果显示,吉马酮在0.058~3.672μg范围内与峰面积呈良好线性关系(R2=1);精密度、稳定性、重复性RSD值分别为0.78%、2.60%、2.90%;平均加样回收率为94.8%,RSD值为4.50%.实验表明,片姜黄现行质量标准可控性不强,而改进后的标准可增强其质量的可控性.     

外科手术配合胆宁片治疗老年胆结石的效果

探讨外科手术配合胆宁片治疗老年胆结石的效果。选择2015年4月-2016年4月于天祝县人民医院诊治的老年胆结石患者89例,根据随机分组法将其分为常规组(43例)和观察组(46例),两组均采用外科手术治疗,观察组在手术治疗的基础上予以胆宁片配合治疗,观察两组患者的治疗效果。观察组总有效率明显高于常规组(P0.05),止痛药使用率与并发症发生率均比常规组低,肛门排气时间与住院天数较常规组更短,且在总治疗费用方面两组无显著差异(P0.05)。在外科手术基础上辅以胆宁片治疗老年胆结石可有效提高患者的治疗效果,促进康复,且并发症少,安全性高。     

电子烟的电磁感应加热雾化技术发展趋势研究

【目的】本研究重点分析新兴的电磁感应加热雾化技术，对其技术发展脉络和研发路线进行剖析，以期为行业提供参考。【方法】通过挖掘专利，对基于电磁感应加热的电子烟技术进行梳理，并对2020年以来的最新技术进行分析。【结果】将前期发展脉络划分为前期技术期和技术稳定期，并对新技术进行归类。【结论】综合表明，电磁感应加热雾化技术在加热性能方面比电阻式加热技术有显著优势，而且不会产生积碳和污垢，具有简单易用、不易损坏的优点，将成为电子烟发展的大趋势。     

云南白药联合妇科千金胶囊治疗宫颈糜烂临床效果研究

探讨云南白药联合妇科千金胶囊治疗宫颈糜烂临床效果。选取2016年1月~2016年12月在定西市安定区第二人民医院治疗的138例宫颈糜烂患者进行分析,随机将其分为常规组(69例)和研究组(69例),常规组患者采取常规基础治疗,研究组患者在此基础上加用妇科千金胶囊联合云南白药,并比较两组患者临床治疗效果以及脱痂时阴道流血量等。治疗后,与常规组患者比较,研究组患者其临床治疗总有效率显著增加,两者间比较差异显著具有统计学意义(P0.05);研究组患者创面脱痂时轻度、中度和重度出血率明显优于常规组,两组比较差异无统计学意义(P0.05)。妇科千金胶囊联合云南白药对宫颈糜烂具有较高的临床效果,且阴道出血量少,该方法值得推广借鉴。     

镍铁钴磷化物纳米片阵列的制备及其电催化析氧性能

采用溶剂热法将镍、铁和钴的硝酸盐溶液在180 ℃条件下反应24 h，经过后续磷化反应，在泡沫镍基底上生长镍铁钴磷化物纳米片阵列(NiFeCoP@NF).采用X射线衍射、扫描电子显微镜、透射电子显微镜、X射线光电子能谱等测试手段对材料进行了表征，并用电化学工作站研究了镍铁钴磷化物纳米片阵列的析氧性能.结果表明:当电流密度达到10 mA/cm2时，需要的过电势仅为160 mV，并且在恒定电流密度下，过电势可保持24 h.研究结果对于研究纳米结构的过渡金属磷化物电催化剂具有借鉴意义.     

碘化N-正丁基氟哌啶醇对大鼠缺氧复氧心肌细胞钠钙交换体电流的抑制作用

碘化N-正丁基氟哌啶醇(N-n-butyl haloperidol iodide,F2)为本研究室改造合成的新化合物。前期研究发现F2作为L-型钙通道拮抗剂,能剂量依赖地拮抗缺血再灌注所导致的大鼠心脏损伤。研究F2对缺氧复氧(hypoxia/reoxygenation,H/R)大鼠心肌细胞钠钙交换体电流的作用并探讨其保护机制。采用Langendorff灌流系统灌流SD大鼠心脏,标准酶解法消化分离得到单个心室肌细胞。正常台式液灌流5min,立即灌流充90%N2-10%CO2的缺氧液,建立体外心肌细胞H/R模型,采用全细胞膜片钳技术记录对照、模型以及不同浓度F2(0.1、1、10μmol/L)对心肌细胞钠钙交换体电流,观察H/R状态F2对心肌细胞钠钙交换体电流的影响。结果显示:缺氧抑制钠钙交换体电流主要是抑制外向电流;H/R引起钠钙交换体电流增大,尤其是外向电流的增大。F2呈浓度依赖地抑制钠钙交换体电流,钠钙交换体电流I-V曲线上移。以上表明:F2能抑制钠钙交换体电流,尤其是外向电流,防止H/R时心肌细胞的钙超载,保护心肌细胞。     

Ion flux dropout observed near dipolarization front

An ion flux dropout near the dipolarization front （DF） at around XGSM =- 11 RE in the Earth＇ s plasma sheet was observed by Time History of Events and Macroscale Interaction during substorms （THEMIS） on March 31, 2009. The ion differential energy fluxes at energies from 450 eV to 150 keV measured by the ESA and SST instruments from THC began to decrease about 2 s before the detection of the DF and reached a local minimum 6 s later. Then, the ion fluxes gradually increased to form a dropout around the DF. The spatial extent of the dropout was about 4,000 km. For energies above 20 keV, the ion fluxes after the dropout are greater than those before it, contrary to the fluxes at energies below 20 keV. The associated ion density variation indicates that the ion flux dropout coincides with the ion density dropout. Taking advantage of multipoint observations, THD, THC, and THE detected the same DF consecutively. Only THC detected an obvious ion flux dropout; THD observed an indistinct one about 2 s before THC; no high-energy （E 〉 30 keV） ion flux dropout was observed by THE. Our study suggests that the ion flux dropout may evolve with the earthward-propagating DF, and its properties can depend on locations relative to the DF.