重核的多核子转移反应和冷碎裂反应

扼要介绍重核的多核子转移反应和冷碎裂反应,指出它们是产生重质量丰中子未知核素的有效途径。     

Green fluorescent protein （GFP） transsenic pig produced by somatic cell nuclear transfer

Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm animals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfecUon system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe- tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h resulted in the highest transfecUon rate （3.6%）. The percentage of GFP reconstructed embryos that de- veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Reconstructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 delivered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research.     

核子湿度密度仪在施工中的应用

<正>郑漯改扩建工程水泥稳定碎石底基层、基层施工中规范要求压实度检测频率为2000m2为6个点,规范要求检测的压实度频率大、工作量大,如果单是靠常规的灌砂法检测现场压实度,     

2022年原子核物理科技热点回眸

作为连接微观粒子物理与介观原子分子物理的桥梁，核科学是一个广泛而多样的学科。从宇宙大爆炸后几个微秒内生成的夸克-胶子等离子体，到质子和中子的形成开始以及元素的合成与演化，再到天体核过程的恒星爆炸和中子星并合，原子核物理学是我们理解宇宙的基础。同时，经过百余年的发展，核物理领域仍有蓬勃的生命力，大量未知现象的发现与科技的发展为人类带来了新的机遇与挑战。简要回顾了2022年原子核物理科技发展的前沿与热点，其中不乏国内引领的优秀工作。而这些方向的突破也为基础科学的发展、国家安全的保障和其他社会应用开辟了新的途径。