精巢间质细胞雄激素合成及其调节的研究进展

本文综述了近年来精巢间质细胞雄激素的合成及其分泌调节的研究进展。间质细胞内的雄激素合成的限速步骤是胆固醇转化为孕烯醇酮时的胆固醇跨线粒体膜转运。在此转运过程中，PBR可能是作为依赖于配体的通道蛋白，对胆固醇分子进行跨膜转移；StAR则通过解离线粒体膜与胆固醇分子的吸附作用，加速了胆固醇分子的转运。雄激素的合成还受垂体、支持细胞、巨噬细胞和其自身分泌激素和细胞因子的调控。同时对今后研究的方向做了总结和展望。     

齿瓣延胡索细胞悬浮系的建立和延胡索乙素的形成

就各种因素对齿瓣延胡索悬浮细胞生长和延胡索乙素形成的影响做进一步的研究。结果表明：随着接种量的提高，细胞生长加速，培养液浊度增大，齿瓣延胡索细胞悬浮液培养中最适接种量为2g/30ml;pH5.6最有利于细胞生物量的积累；2，4—D与2种细胞分裂素的组合，对于生长量积累和THP含量的作用最显著；在培养基中添加甘露糖和半乳糖，对于提高培养物THP含量是有效的；G1y Phe Lys的作用效果最显著，细胞鲜质量、干质量和THP含量均最高。     

铂(II)与胺类配合物的合成、晶体结构和抗肿瘤活性研究

研究铂(II)与三种胺类配合物的合成、晶体结构和体外抗肿瘤活性,化合物1的X-射线单晶结构分析表明:这是一个很少见的具有四方锥几何构型的铂(II)配合物,顺式平面四方形配位的铂的轴向上有第5个配位原子。其抗肿瘤活性实验显示3个配合物对几种研究的癌细胞都有较强的抑制作用。     

Some Properties of Fractals Generated by Linear Cellular Automata

Fractals and cellular automata are both significant areas of research in nonlinear analysis. This paper studies a class of fractals generated by cellular automata. The patterns produced by cellular automata give a special sequence of sets in Euclidean space. The corresponding limit set is shown to be a fractal and the dimension is independent of the choice of the finite initial seed. As opposed to previous works, the fractals here do not depend on the time parameter.     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

口服转胸腺素α1基因聚球藻对小鼠T细胞亚群作用的研究

为探讨口服后转胸腺α1(Tα1)基因聚球藻对T细胞亚群的作用,将小鼠随机分为空白对照组、野生藻组、转化藻(小剂量、中剂量和大剂量)组和日达仙组,用灌服的方式,给予空白对照组20mL·kg~(-1)的生理盐水,野生藻组0.4g·kg~(-1)的野生聚球藻;转化藻小、中、大剂量组,分别0.2g·kg~(-1)、0.4g·kg~(-1)和0.6g·kg~(-1)的转Tα1基因聚球藻;日达仙组则肌注给予3.2mg·kg~(-1)的胸腺素α1(日达仙)。结果表明转Tα1基因藻口服后可显著提高CD3亚群水平,显著提高CD4亚群水平,明显提高CD4/CD8的比值。提示转Tα1基因聚球藻具有增强机体免疫功能的作用。     

基于广义归纳逻辑因果模型的知识发现与算法实现

以语言场、广义细胞自动机和广义归纳逻辑因果模型为理论依据，分析了广义因果联系类知识的发现机理，给出了因果联系类知识发现的实现算法。该算法为解决具有随机不确定和模糊不确定性特征的因果联系类知识的发现提供了行之有效的方法。通过算法的运行实例，验证和说明了算法的正确性和有效性。     

明矾对蚕豆和大蒜根尖毒性效应的细胞遗传学比较研究

以蚕豆和大蒜根尖为实验材料，研究不同浓度明矾对蚕豆和大蒜胚根根尖的细胞遗传学效应。结果表明，不同浓度明矾在不同处理时间内均能诱发较高微核率和染色体畸变率，且在同样的条件下，蚕豆根尖细胞的微核率和染色体畸变率明显高于大蒜的(p＜0．01或p＜0．001)。结论是明矾对蚕豆和大蒜根尖均具有明显的细胞遗传学务性效应，且对蚕豆根尖的务性效应明显高于对大蒜根尖的毒性效应。