ABA、6—BA对质膜破坏的影响研究

以小麦(Tricua aestivum)离体叶片为材料,通过衰老处理,研究其衰老过程中质膜的分解与破坏。结果表明:经脱落酸(ABA)处理的叶片,其超氧化物歧化酶(SOD)活性、抗坏血酸(Vitam C)含量下降加剧,丙二醛(MDA)迅速积累,质膜透性丧失加快,膜破坏迅速;经6-苄氨基嘌呤(6-BA)处理,其SOD活性、Vitam C含量下降减缓,MDA缓慢积累,质膜透性丧失减慢,膜破坏减缓。     

对小麦杂种优势研究方法的探讨

目前小麦品种问的遗传相似系数达0．8以上,造成杂种优势幅度降低,缺乏强优势组合,因此,迫切需要拓宽小麦遗传基础和扩大基因多样性。利用杂种优势是提高小麦单产的重要途径之一。本文分析了小麦杂种优势利用方法。     

Sm-6229菌剂对小麦幼苗生长的影响

采用０．１％、０．２％的Ｓｍ－６２２９菌剂浸种和喷施小麦幼苗,并观测其幼苗的生长与生理变化、结果表明：菌剂对小麦种子萌发、幼苗生长、以及对叶绿素、蛋白质和可溶性糖含量等均有促进作用,尤其是０．２％的菌剂浸种,效果较明显,作为一种促进植物工的物质,可和于农业生产。     

抗黄矮病小麦易位系cDNA文库的构建

对小麦-中间偃麦草易位系HW642一周龄幼苗接种饲黄矮病毒蚜虫,接种48 h后取幼叶提取mRNA构建了一个cDNA文库,文库的滴度为1.5×105pru/mL.随机挑取103个重组克隆,用限制内切酶EcoR I、Xho I消化,统计分析结果表明插入片段在150～4000 bp之间,平均插入片段大小为2000 bp左右,重组率为95.15 ％.文库可用于筛选抗病候选基因及EST研究等.     

Molecular dissection of plant height QTLs using recombinant inbred lines from hybrids between common wheat （Triticum aestivum L.） and spelt wheat （Triticum spelta L.）

In wheat, plant height is an important agronomic trait, and a number of quantitative trait loci (QTLs) controlling plant height have been located. In this study, using the conditional and unconditional QTL mapping methods, combined with data from five different growth stages over two years of field trials, the developmental behavior for plant height in wheat was dissected. Nine unconditional QTLs and 8 conditional QTLs were identified, of which 6 were detected by both methods. None of the 11 QTLs was detected at all of the 5 investigated developmental stages, but 7 QTLs were detected at certain stages in both years. Further analysis identified 9 unconditional QTLs at different stages, which could explain the phenotypic variation from 4.81% to 17.35%. It was noteworthy that one major QTL designated QHt-4B-2, which was located on chromosome 4B, was detected on May 18 and 25 in both years, and its genetic contributions to plant height ranged from 13.42% to 16.13%. Moreover, of the 8 conditional QTLs identified, six were detected in both years, in the order of QHt-3B→QHt-4B-1→QHt-4B-2→QHt-4D→QHt-5A and QHt-2B expressed at the same developmental stage. The results indicate that QTL expression during plant height development is selective and in a temporal order.     

添加物对黑小麦草青贮的细胞壁成分和体外干物质消化率的影响及其与小麦、黑麦草青贮的比较

用乳熟期的黑小麦草、小麦及黑麦草调制了青贮，每种草都有添加乳酸菌(LAB)、纤维素酶(CEL)、乳酸菌 纤维素酶(MIX)和对照(CON)4种处理，常温下贮存49天开封后，测定了青贮的细脓壁成分(OCW—总纤维、Ob—低消化性纤维、Oa—高消化性纤维)含量和体外干物质消化率（IVDMD)．结果表明，添加CEL和MIX使所有青贮的OCW、Ob含量和黑小麦草青贮的Oa含量减少，添加CEL使小麦青贮的IVDMD提高；黑小麦草青贮的OCW和Ob含量高于小麦青贮低于黑麦草青贮，Oa含量低于其他两种青贮，IVDMD低于小麦青贮，极显著地高于黑麦草青贮．