Loss of antibody production accompanied by chromosome loss in a cloned hybrid line secreting antibodies to sheep red blood cells

Summary Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG₁ antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascitec fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody.     

Juvenile hormone titre and regulation in the cockroachDiploptera punctata

Summary Titres of juvenile hormone (JH) have been determined in both hemolymph and whole body extracts of femaleDiploptera punctata during the first gonotrophic cycle using a method employing gas chromatography/mass spectrometry for qualitative and quantitative analysis. JH III is the sole JH found in both adult and last instarD. punctata. Maximum values of 1500 ng/ml (6M) were observed at the middle of the gonotrophic cycle, when basal oocyte growth rate was greatest. Changes in rates of JH release in vitro by corpora allata paralleled closely the changes in JH titre, suggesting that biosynthesis is a major regulator of titre. JH levels per animal were calculated from observed JH titres, and at certain time points in the gonotrophic cycle JH levels obtained from analysis of whole bodies were significantly greater than those predicted from hemolymph titres. These results suggest the existence of a nonhemolymph JH pool inD. punctata. Decay in JH titre after allatectomy of 5 day females has also been studied. Following a rapid initial decline, the rate of decay slowed appreciably 4 h post-operation. Thus, use of a first-order rate constant to estimate half-life of JH significantly underestimated the longevity of the hormone. The apparent persistence of JH following allatectomy may be due to the existence of a nonhemolymph JH pool.     

煤炭企业固定工的地位及现存问题的改进

本文对当前煤炭企业用工制度改革存在的问题、改进途径和各种形式用工的比例进行了分析,并指出为适应煤矿安全生产及机械化发展的需要,保持固定工的用工比例,克服“铁饭碗在手”等隋性意识,充分发挥他们的骨干作用是问题的关键。为此,本文提出了改变工资结构、劳动结构等相应的改革措施。     

基于水光电力系统时序生产模拟模型的水光优化配比研究

基于考虑系统负荷、新能源发电出力等时序变化特性的时序生产模拟法,建立了水光电力系统时序生产模拟模型;从系统结构性、经济性、灵活性与可靠性方面综合确定最佳水光容量配比,并通过金沙江上游川藏段水光系统验证了模型的合理性。金沙江上游川藏段光伏电站与水电站出力具有较好的年内、日内互补特性;对于2030年水平年,考虑枯期水电留存,913.6万kW水电配套400万kW光伏的方案综合性能更优,其新增非水可再生电量约60亿kW·h。     

一类两种群竞争模型的定性分析

对两种竞争模型｛ｘ＝ｘ（ａ－ｂｘ－ｃｙ－ｋｘｙ）＝Δｘｆ１（ｘ,ｙ）;ｙ＝ｙ（ｅ－ｆｘ－ｇｙ－ｌｘｙ）＝Δｙｆ２（ｘ,ｙ）,的平衡点的全局稳定性和极限环的存在性作定性研究,得到了正平衡点全局稳定的充分条件,证明了该系统在第一象限内不存在极限环。     

批量制造业模糊交货期准时化生产计划方法

通过建立批量制造业模糊交货期下的准时化生产计划模型,将隶属度函数引入到生产计划问题中,从而拓宽了准时化生产计划问题的研究范围,并为实际生产计划的编制提供了一种有效途径。     

一种使用非线性循环数列解决堆栈问题的算法

提出了一种使用非线性循环数列解决堆栈问题的算法,对研究栈的性质和求解栈的输出序列具有一定的实用价值。     

基于分散网络化生产系统的车间生产管理模式及系统设计研究

基于分散网络化生产系统,针对我国中小型机械制造企业技术现状,提出了在基本上不改变现有车间基础设施的前提下,通过信息集成建立产品数据,财务和计算机辅助车间调度等管理子系统,以增强企业适应产品市场多变的能力;采用多品种,小批量生产方式,以满足产品生命周期缩短和２１世纪国际化大市场竞争的需求。     

我国东南低山丘陵区土地粮食生产潜力研究──以桂林市为例

我国东南低山丘陵区资源丰富,历来为重要的粮食生产基地．但其粮食生产近年发展缓慢．根据采用趋势潜力与资源潜力相结合的方法,所进行的桂林市土地粮食生产潜力及其发挥和实现影响因素的研究结果,该区粮食生产潜力较大,但目前发挥和实现程度低．为促进粮食生产潜力的发挥．并保证其持续发展．今后应大力改造中产田．提高土地质量和耕地灌溉保证率．加强粮食生产的物化投入,保证活劳动投入的需要,并同时注意协调化肥的投入结构．     

福州陶江中国淡水蛏Novaculina chinensis生殖周期的研究

采用活体性腺观察和组织切片法对福建闵候尚干陶江中国淡水蛏的生殖腺进行了初步研究,淡水蛏的性腺发育分为５期,增殖期,生长期,成熟期,排放期和休止期,一年只有一个繁殖周期,繁殖期为３月下旬至４月,雌雄异体,有雄性早熟现象,较晚期的卵母细胞和成熟卵子有双质核仁现象。