图像拓扑结构的识别及其应用技术研究

快速准确地建立两图像拓扑结构之间的对应关系是要解决的核心问题。通过揭示拓扑结构的本质特性,提出了邻接矩阵模型、局部模型和快速模型三种不同的拓扑结构的识别模型、数据结构及其算法,重点讨论了快速模型,并对其优良性能进行了证明。最后,把快速模型运用于全景图拼接,取得了令人满意的实验结果。     

第四代海事卫星通信陆地移动终端天线阵的设计

为解决圆极化微带天线阵馈电网络较为复杂、工程实现较为困难这一问题,提供一种适用于宽带全球局域网陆地移动终端的天线阵简易设计方法.采用微带线三维过渡段倾斜馈电,利用方形贴片切角实现圆极化工作,并采用叠层单元结构连续旋转组成天线阵,使得天线的阻抗带宽(VSWR≤2)和轴比带宽(AR≤3 dB)分别达到35%和20%.实测结果表明,天线在整个海事卫星工作频带内驻波比小于1.5,增益高于13 dB.实测结果与仿真结果具有良好的一致性.     

轴对称问题中的无网格Galerkin法

采用无网格Galerkin法分析轴对称问题，得到弹性力学中的对称问题的无网格离散方程．将这一方法与有限元耦合，即在边界处布置有限单元，这样就可以用传统有限元方法方便地处理力学边界条件．算例考察表明：本文方法通过了分片检验，计算结果达到了较高的精度，最大误差不超过5％．     

大鼠肾上腺嗜铬细胞原代培养的一种实用方法

以胶原酶Ⅰ、透明质酸酶和ＤＮａｓｅ联用消化肾上腺髓质，再经两次离心收集嗜铬细胞，建立了大鼠肾上腺嗜铬细胞的分离制备及原代培养的实用方法．经反复实验（ｎ＝１２）证明，所获得的细胞形态良好，功能正常．细胞表面光洁、完整，呈球形，直径在８～１２μｍ范围．用细胞贴附式和全细胞方式膜片钳技术在培养２～７ｄ的嗜铬细胞上记录到多种电压敏感性跨膜离子电流，说明培养细胞的膜离子通道完整，适于细胞纯度要求不很高（＜９５％）的单个细胞实验．     

2-APB对Wistar大鼠脑微动脉平滑肌细胞间缝隙连接的抑制作用

为探讨2-APB对Wistar大鼠脑微动脉平滑肌细胞间缝隙连接的作用。采用全细胞膜片钳技术,观察2-APB对Wistar大鼠脑微动脉段上平滑肌细胞膜电容(Cinput)、膜电导(Ginput)和膜电阻(Rinput)的影响。结果显示,应用2-APB后Wistar大鼠脑动脉段平滑肌细胞的Cinput、Ginput减小或Rinput增大。当2-APB浓度≥100μmol/L时Wistar大鼠脑微动脉段上平滑肌细胞的Cinput、Ginput分别从81±18 pF和3.5±0.43 nS降至10.5±1.3pF(n=7,P0.01)和0.35±0.03nS(n=7,P0.01),这与单个平滑肌细胞十分接近。由此可知,2-APB可以浓度依赖性的抑制Wistar大鼠脑微动脉平滑肌细胞间缝隙连接。     

Calyculin a increases voltage-dependent inward current in,smooth muscle cells isolated from guinea pig taenia coli

The effects of a potent phosphatase inhibitor, calyculin A (CL-A), on inward currents in guinea pig taenia coli smooth muscle cells were examined. CL-A increased the inward current, and this effect of CL-A was inhibited by a protein kinase C inhibitor, H-7, and by nifedipine. Phorbol 12,13-dibutyrate, an activator of protein kinase C, also increased the inward current and this effect was antagonized by H-7. These results suggest that in guinea pig taenia coli smooth muscle cells CL-A may facilitate the opening of thel-type Ca²⁺ channels through the protein kinase C-dependent phosphorylation system.     

Two serotonin-sensitive potassium channels in the identified heart excitatory neurone of the African giant snail,Achatina fulica Fèrussac

Summary By the patch clamp experiments, two serotonin-sensitive K⁺ channels (SL-channel and SS-channel) were demonstrated in the identified heart excitatory neurone (PON) of the African giant snail,Achatina fulica Fèrussac. The activities of both channels could be recorded in the steady state and those activities disappeared on application of 5-HT.     

Patch and whole-cell voltage clamp of single mammalian visceral and vascular smooth muscle cells

Summary Dispersal of the constituent cells of mammalian visceral and vascular smooth muscles has permitted recordings both of membrane currents under whole-cell voltage clamp, and of currents through single ionic channels using the patch-clamp technique. A rectangular depolarizing step applied to a single cell under voltage clamp yielded a net inward current followed by a net outward current in normal physiological solution. In isolated, inside-out patches of cell membrane a calcium- and potential-sensitive K channel (100 pS conductance) and a calcium-insensitive, potential-sensitive K⁺ channel (50 pS conductance) with slow kinetics have so far been identified and characterized.     

Rat pancreatic islet cells in primary culture: occurrence of giant cells amenable to patch clamping

Summary Neonatal and adult rat pancreatic islet cells were maintained in dissociated cell culture for up to three weeks. The unexpected occurrence of giant (40–50 m) cells was noted, some of which reacted positively to an insulin antiserum, indicating the presence of insulin. The giant cells were amenable to study using the extracellular patch clamp technique, which was used to demonstrate a population of membrane channels gating outwardly directed current in these cells.     

蒙特卡罗方法研究集合种群动态

根据金堇蛱蝶的实际生活史和调查数据,以天为单位采用蒙特卡罗模拟方法仿真每个个体的发育、迁移、繁殖等随机行为. 从而获取集合种群及其各局域种群的动态变化,展示了局域种群的周转行为,得到了每年各斑块局域种群大小、占据率、被占据斑块数及相应的概率分布等信息. 计算结果表明该集合种群能够进入一个基本稳定的状态从而维持适当的种群规模并得以续存下去.我们还分析了斑块质量和所处地理位置对局域种群的影响. 提供了一种能够同时描述集合种群和局域种群动态的研究方法,为将来进一步研究各种影响集合种群动态的因素和制定保护策略等奠定了基础.