高效液相色谱测定人尿中异硫氰酸酯方法的优化

优化了反相高效液相色谱法测定人尿液中异硫氰酸酯含量的方法。本法适合大批样品测定人尿液中异硫氰酸酯．     

一种改进的相对轨道动力学模型

线性化的Clohessey-Whiltshire(C-W)方程描述相对运动虽然方便,但是精度不高,为了解决这一问题,提出了一种C-W方程的改进形式。首先基于C-W方程的推导,分析了在对中心引力取近似的过程中导致原方程不准确的数学原因和物理现象;然后通过对简化部分的数量级进行分析和比较,将原推导过程中忽略掉的部分重要内容重新予以考虑,并运用数学变换将新加入的内容合并转化,最终给出了一组非齐次线性常系数微分方程,在不增加计算复杂度的情况下提高了精度。仿真验证表明该方程在圆轨道下相对于C-W方程精度改善很多,小椭圆轨道下也消除了C-W方程的长期误差。     

抛物型方程初值问题的概率数值解法

Ｆｒｉｅｄｍａｎ在［１］中给出了一般抛物型方程Ｃａｕｃｈｙ问题解的概率表达式。在此基础上，给出了它的概率数值及其误差估计。     

扩散过程样本的Holder连续性及其应用

本文讨论扩散过程样本的Ｈｏｌｄｅｒ连续性，在局部Ｌｉｐｓｃｈｉｔｚ条件下，我们证明了扩散过程样本类似于Ｂｒｏｗｎ运动样本一样的Ｈｏｌｄｅｒ连续性，进一步，我们还了扩散过程样本的象与图集的Ｈａｕｓｄｒｏｆｆ维数。     

Storage of irradiated human blood; a source of error in quantitative chromosome analysis

Summary Human whole blood was irradiated with 2.5 Gy of 220 kVp X-rays and stored before culture with 9.7 M BrdU and 19.4 or 38.7 M BrdU for 0, 24, 48 and 72 h. The frequency of dicentrics and ring chromosomes was determined in cells staining as first division (M1) metaphases with the fluorescence plus Giemsa technique. Storage had no influence on the observed aberration yields in 44 h cultures containing 9.7 M BrdU. In 66 h cultures at 19.4 M BrdU the observed yields after 2 and 3 days' storage were significantly lower as compared to cultures from fresh blood. No storage effect was revealed in 66 h cultures containing 38.7 M BrdU. In cases where cytogenetic radiation dosimetry has to be carried out using blood samples which have been in transit for 2–3 days, the findings are of relevance for a correct determination of the chromosome damage in M1 cells.     

Self-labeling of human polymorphonuclear leucocyte myeloperoxidase with125iodine

In order to obtain a radioimmunoassay (RIA) technique for the measurement of human plasma myeloperoxidase (MPO), we purified the enzyme from polymorphonuclear granulocytes (neutrophils), and compared three methods of labeling it with¹²⁵Iodine: chloramine T, lactoperoxidase, and an original technique of self labeling based on the ability of the enzyme to oxidize and bind¹²⁵I in the presence of H₂O₂. The chloramine T technique produced a degraded protein, as well shown by a high non-specific binding of tracer to antibody. The lactoperoxidase technique did not succeed in labeling MPO with an adequate specific activity. In contrast, the self-labeling method gave a stable tracer with a specific activity of 23 Ci/gmg MPO (85 MBq), a satisfactory level of immunoreactivity, and a low-specific binding (3%). After labeling, purification of tracer was achieved by gel filtration chromatography in phosphate buffer (0.05 M; pH7) to which 0.1% poly-L-lysine was added. The labeled molecule remained stable for 40 days and could be used for RIA with a polyclonal antibody raised in rabbits.     

Thrombospondin co-localises with TGFβ and IGF-I in the extracellular matrix of human osteoblast-like cells and is modulated by 17β estradiol

Thrombospondin (TSP) is a multifunctional glycoprotein which is synthesised by several cell types including osteoblasts, and incorporated into the extracellular matrix (ECM) of these cells. The function and regulation of TSP in bone is not clear. In this study, using a long term culture model of human osteoblast-like cells, we examined the distribution of TSP in the ECM and its modulation by added estradiol. In this model the osteoblast-like cells form a regular multilayer which continues to increase in depth up to 50 days post confluence. In the ECM of these cultures and in 19-week fetal bone, the bone markers osteocalcin and alkaline phosphatase were diffusely distributed in the matrix. In contrast, labelling for TSP was concentrated, confined to the banded collagen and its immediately adjacent ECM. This pattern of labelling resembled that of the growth factors transforming growth factor-I (TGF), and insulin-like growth factor-I (IGF-I), with which TSP label co-localised. Labelling intensities were comparable between fetal bone and the in vitro material for TSP, TGF and IGF-I. TSP label was present by 10 days post confluence, reached a maximum by 20 days, and declined slowly thereafter, a time course which was similar to that of IGF-I. Incubation of osteoblast-like cell cultures with 17 estradiol resulted in an increase in multilayer depth and a maximal 3-fold increase in TSP labeling at 30 days as well as approximately 2-fold increases for TGF and IGF-I. The dose-response relationship for these responses to estradiol treatment was biphasic with maximal increases at 10^–10 M–10^–11 M of added estradiol. Treatment with 17 estradiol produced labelling intensities that were not significantly different from controls. Studies with other cell types have suggested that TSP may be involved in modulation of growth factor activity. The similarities between TSP, TGF and IGF-I, in terms of their distribution and regulation by 17 estradiol treatment, may indicate a role for TSP in modulating bone cell proliferation and function through interaction with local growth factors.     

基于逻辑相似性的运动检索算法

针对基于内容的人体运动检索问题,提出了一种基于逻辑相似性的运动数据检索方法。首先,针对特定人体运动序列数据,使用自组织网络映射算法对运动数据进行预处理,找出运动数据帧的最佳匹配单元。然后,应用数据编码规则对匹配单元进行量化及编码处理,获得基于符号化表示的运动描述符。基于符号化表示的描述符建立运动序列索引机制,应用多分辨率框架进行运动模式匹配。最后,按照模式匹配结果进行运动相似性排序,输出检索结果。在CMU运动数据库中的试验结果表明了算法有效性。