Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

Expression of human clotting factor Ⅸ mediated by recom- binant lentiviral vector in cultured cells and hemophilia B mice

To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driven by ubiquitin-C promoter, FUXW, and by ABP liver specific promoter, FAXW, was constructed respectively. Recombinant lentivirus was harvested from 293T cells by calcium phosphate-mediated transient cotransfection of three plasmids (transgene vector, CMV腞8.2, VSV-G). hFⅨ expression was detected in supernatant of 293T, BHK and L-02 cells infected with FUXW virus, whereas higher expression of hFⅨ levels (630 ng/106 cells/48 h) was detected only in L-02 cells infected with FAXW virus. Serum hFⅨ antigen was detected in all hemophilia B mice treated with FAXW virus by tail vein injection, an efficiency level of hFⅨ was observed (45 ng/mL, approximately 1% of normal human levels), the expression lasted for more than 60 d. The results indicated that HIV-based lentiviral vectors offer a promising approach to the gene therapy of hemophilia B.     

DNA分子标记在果树上的应用

介绍了在果树上常用的DNA分子标记的原理和特点，并对其在果树的分子遗传图谱和指纹图谱的构建，品种鉴别、系谱分析和分类，基因定位与分子标记辅助选择等方面的应用进行了综述。     

口服转胸腺素α1基因聚球藻对小鼠T细胞亚群作用的研究

为探讨口服后转胸腺α1(Tα1)基因聚球藻对T细胞亚群的作用,将小鼠随机分为空白对照组、野生藻组、转化藻(小剂量、中剂量和大剂量)组和日达仙组,用灌服的方式,给予空白对照组20mL·kg~(-1)的生理盐水,野生藻组0.4g·kg~(-1)的野生聚球藻;转化藻小、中、大剂量组,分别0.2g·kg~(-1)、0.4g·kg~(-1)和0.6g·kg~(-1)的转Tα1基因聚球藻;日达仙组则肌注给予3.2mg·kg~(-1)的胸腺素α1(日达仙)。结果表明转Tα1基因藻口服后可显著提高CD3亚群水平,显著提高CD4亚群水平,明显提高CD4/CD8的比值。提示转Tα1基因聚球藻具有增强机体免疫功能的作用。     

鄂尔多斯蒙古族、汉族5项遗传学指标研究

调查了内蒙古鄂尔多斯蒙古族(508例)、汉族(461例)人的5项头面部形态特征.5项指标是鼻梁侧面观、鼻孔形状、下颏突出度、额头发际、头发形状.调查显示:(1)这两个人群多为非凸型鼻梁、宽型鼻孔、非突型下颏、直发,额头发际有尖率与无尖率接近.(2)在内蒙古诸人群中,这两个人群凸鼻梁率低、宽型鼻孔率高、突型下颏率低、额头发际有尖率高、直发率低.(3)5项指标彼此多不相关,仅鼻梁类型与鼻孔形状相关,鼻梁类型与下颏类型相关.     

具有局部搜索能力的自适应变异遗传算法

针对函数优化问题，提出一种自适应变异遗传算法来提高局部搜索的能力，弥补简单遗传算法易于早熟收敛的缺陷。最后以De Jong函数为仿真对象，将此算法与其它三种遗传算法进行比较，仿真结果表明此算法对于函数优化问题非常有效，大大加快了算法的收敛速度，并大幅度提高了搜寻到最优解的概率。     

基于基因算法的模糊滤波器的研究

对基因算法的模糊识别进行了研究。对于简单自适应模糊滤波器不能很好地解决由于信息冗长带来的局部最优问题 ,可采用基因算法进行优化。其在不牺牲学习效率的前提下可以获得良好的性能。     

种植器官的“农场”

詹姆士·芬恩已被逼到了绝境。帕金森氏症正在吞噬他的运动和语言能力,而且所有常用药物都无济于事。因此当医生向他提出一种试验性治疗的建议时,他决定试试运气。1996年9月24日,医生在他的头骨上凿了一个洞,植人数百万个神经元,以取代其大脑已经停止制造的一种化学物质。但这些植入物的来源却非同寻常:它们来自一头猪。     

转基因食品的安全性与发展前景

欧美关于转基因食品是否安全的争论闹得沸沸扬扬。转基因食品生产和输出大户美国,从其自身利益出发,主张只要科学上无法证明它有害,就不应该限制。而转基因食品输入最多的欧洲国家,公众对转基因食品普遍感到恐慌;他们认为,一些动物实验显示,某些转基因食品对动物有害,而对人类如何由于时间短还不清楚,但只要不能否定其危险性,就应该加以限制,二者针锋相对。为此,2000年2月28日在英国爱丁堡举行了一次有关基因改性食品安全的国际论坛,会议呼吁人们对此类具有革命性但尚有争议的产品保持谨慎,并予以合理评价;2002年“3·15”,国际消费者联合会…     

视黄醇结合蛋白基因的研究发展

视黄醇结合蛋白(Retinol-Binding Proteins，RBP)是一类维生素A的运载蛋白，参与血清和细胞内视黄醇／酸的转运，是疏水小分子结合蛋白家族的成员。RBP主要在肝脏中合成并释放入血液进而进入各种组织，通过与视黄醇、前白蛋白及细胞表面受体相互作用，在维生素A的储存、代谢、转运到周围靶器官中具有重要功能。细胞内视黄醇结合蛋白则主要在细胞内发挥类似作用。本文主要就血清及细胞内视黄醇结合蛋白的基因结构、作用机理、发育性表达、组织定位及染色体定位、对动物繁殖性能和胚胎发育的影响等方面进行综述。