全文获取类型
收费全文 | 157篇 |
免费 | 1篇 |
专业分类
系统科学 | 3篇 |
现状及发展 | 33篇 |
研究方法 | 33篇 |
综合类 | 73篇 |
自然研究 | 16篇 |
出版年
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2013年 | 4篇 |
2012年 | 7篇 |
2011年 | 31篇 |
2010年 | 2篇 |
2008年 | 5篇 |
2007年 | 9篇 |
2006年 | 10篇 |
2005年 | 12篇 |
2004年 | 13篇 |
2003年 | 11篇 |
2002年 | 9篇 |
2001年 | 3篇 |
2000年 | 6篇 |
1997年 | 1篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1974年 | 1篇 |
1971年 | 1篇 |
1970年 | 1篇 |
1968年 | 1篇 |
1959年 | 1篇 |
1948年 | 2篇 |
1947年 | 6篇 |
1946年 | 4篇 |
1945年 | 1篇 |
排序方式: 共有158条查询结果,搜索用时 578 毫秒
101.
Patterns of mineral nutrient uptake and distribution within the roots, stems, and leaves of Artemisia pygmaea and in the vascular parasite Orobanche fasciculata were investigated. All nutrients studied were magnified over concentrations found in the soil into the host and parasite. Nitrogen, phosphorus, potassium, and zinc were magnified along the flow gradient of soil-roots-stems-leaves of the host. All others increased in the roots and then decreased in the stems and leaves. Orobanche fasciculata concentrated phosphorus, potassium, and sodium over soil and root concentrations while excluding to some degree all others. 相似文献
102.
Craig Groves Terry Frederick Glenn Frederick Eric Atkinson Melonie Atkinson Jay Shepherd Gregg Servheen 《西北部美国博物学家》2011,57(2)
From 1990 to 1992 we surveyed for Flammulated Owls ( Otus flammeolus ) in 3 areas in Idaho: Salmon National Forest (SNF), Payette National Forest and adjacent Hells Canyon National Recreation Area (PNF-HCNRA), and Nez Perce National Forest (NPNF). We also collected and summarized information on all historic and modern records of Flammulated Owls in Idaho. Flammulated Owls were detected on 65% of 68 routes (2-16 km in length) surveyed at densities ranging from 0.04 to 1.25 singing males/40 ha. Owls were detected on survey routes as early as 10 May and as late as 23 July. Mean percent canopy cover estimated at owl locations on the PNF-HCNRA and NPNF study sites ranged from 52% to 64%, while shrub cover ranged from 16% to 21% and ground cover was 39% to 49%. Our surveys and summary of distributional records indicated that Flammulated Owls occur throughout the montane forests of Idaho in old or mature stands of open ponderosa pine ( Pinus ponderosa ), Douglas-fir ( Pseudotsuga menziesii ), and stands co-dominated by those 2 species. Fire suppression and timber harvest activity in ponderosa pine forests represent 2 main threats to the species' future security in Idaho. More research on the effects of various silvicultural treatments on Flammulated Owl populations is warranted. 相似文献
103.
Ruffed Grouse ( Bonasa umbellus ) population densities are lower in the Intermountain West than elsewhere in the species' range. Throughout much of its range, the Ruffed Grouse is closely associated with quaking aspen ( Populus tremuloides ), in part because aspen buds are an important winter food. Because population fluctuations of Ruffed Grouse have been associated with changes in aspen abundance or chemical composition, we studied winter foraging of the species in the Intermountain West where it has received little attention. Aspen buds were the most prominent forage in the bird's diet, although in contrast to other Ruffed Grouse food habits studies, reproductive buds were not eaten more than vegetative buds, and buds of other deciduous plants were also important (>20% of the diet). Excretion of high concentrations of ammonium nitrogen suggests that grouse in northern Utah are ingesting higher levels of secondary plant compounds than reported elsewhere. Our results show aspen is important in the winter ecology of Ruffed Grouse in northern Utah and suggest that continued loss of aspen may impact grouse populations. 相似文献
104.
Mutation of BSND causes Bartter syndrome with sensorineural deafness and kidney failure. 总被引:21,自引:0,他引:21
R Birkenh?ger E Otto M J Schürmann M Vollmer E M Ruf I Maier-Lutz F Beekmann A Fekete H Omran D Feldmann D V Milford N Jeck M Konrad D Landau N V Knoers C Antignac R Sudbrak A Kispert F Hildebrandt 《Nature genetics》2001,29(3):310-314
Antenatal Bartter syndrome (aBS) comprises a heterogeneous group of autosomal recessive salt-losing nephropathies. Identification of three genes that code for renal transporters and channels as responsible for aBS has resulted in new insights into renal salt handling, diuretic action and blood-pressure regulation. A gene locus of a fourth variant of aBS called BSND, which in contrast to the other forms is associated with sensorineural deafness (SND) and renal failure, has been mapped to chromosome 1p. We report here the identification by positional cloning, in a region not covered by the human genome sequencing projects, of a new gene, BSND, as the cause of BSND. We examined ten families with BSND and detected seven different mutations in BSND that probably result in loss of function. In accordance with the phenotype, BSND is expressed in the thin limb and the thick ascending limb of the loop of Henle in the kidney and in the dark cells of the inner ear. The gene encodes a hitherto unknown protein with two putative transmembrane alpha-helices and thus might function as a regulator for ion-transport proteins involved in aBS, or else as a new transporter or channel itself. 相似文献
105.
Recent discoveries of native cutthroat trout populations in desert mountain ranges on the western fringe of the Bonneville Basin have prompted intensified management efforts by state and federal agencies. Analysis of Snake Valley cutthroat specimens in Trout Creek, Deep Creek Mountain Range, Utah, indicate this is a pure strain of the trout which once inhabited Pleistocene Lake Bonneville and which was thought to be extinct in Utah. The Snake Valley cutthroat is similar to Salmo clarki utah of the eastern Bonneville Basin; however, electrophoretic and morphomeristic analysis show unique genetic differences brought about by long - term isolation (8,000 years) from the remainder of the Bonneville Basin cutthroat. This cutthroat is a common ancestor to several other limited cutthroat populations within the basin in Nevada. In May 1977 the BLM withdrew from mineral entry about 27,000 acres within the Deep Creek Mountains for protection of this salmonid cutthroat and other unique resources on the range. Results of 1977 stream surveys on the Pilot Peak Mountain Range, Utah, indicate the presence of the threatened Lahontan cutthroat, Salmo clarki henshawi, in one isolated stream. 相似文献
106.
107.
Reysenbach AL Liu Y Banta AB Beveridge TJ Kirshtein JD Schouten S Tivey MK Von Damm KL Voytek MA 《Nature》2006,442(7101):444-447
Deep-sea hydrothermal vents are important in global biogeochemical cycles, providing biological oases at the sea floor that are supported by the thermal and chemical flux from the Earth's interior. As hot, acidic and reduced hydrothermal fluids mix with cold, alkaline and oxygenated sea water, minerals precipitate to form porous sulphide-sulphate deposits. These structures provide microhabitats for a diversity of prokaryotes that exploit the geochemical and physical gradients in this dynamic ecosystem. It has been proposed that fluid pH in the actively venting sulphide structures is generally low (pH < 4.5), yet no extreme thermoacidophile has been isolated from vent deposits. Culture-independent surveys based on ribosomal RNA genes from deep-sea hydrothermal deposits have identified a widespread euryarchaeotal lineage, DHVE2 (deep-sea hydrothermal vent euryarchaeotic 2). Despite the ubiquity and apparent deep-sea endemism of DHVE2, cultivation of this group has been unsuccessful and thus its metabolism remains a mystery. Here we report the isolation and cultivation of a member of the DHVE2 group, which is an obligate thermoacidophilic sulphur- or iron-reducing heterotroph capable of growing from pH 3.3 to 5.8 and between 55 and 75 degrees C. In addition, we demonstrate that this isolate constitutes up to 15% of the archaeal population, providing evidence that thermoacidophiles may be key players in the sulphur and iron cycling at deep-sea vents. 相似文献
108.
Blaga CI Xu J DiChiara AD Sistrunk E Zhang K Agostini P Miller TA DiMauro LF Lin CD 《Nature》2012,483(7388):194-197
Establishing the structure of molecules and solids has always had an essential role in physics, chemistry and biology. The methods of choice are X-ray and electron diffraction, which are routinely used to determine atomic positions with sub-?ngstr?m spatial resolution. Although both methods are currently limited to probing dynamics on timescales longer than a picosecond, the recent development of femtosecond sources of X-ray pulses and electron beams suggests that they might soon be capable of taking ultrafast snapshots of biological molecules and condensed-phase systems undergoing structural changes. The past decade has also witnessed the emergence of an alternative imaging approach based on laser-ionized bursts of coherent electron wave packets that self-interrogate the parent molecular structure. Here we show that this phenomenon can indeed be exploited for laser-induced electron diffraction (LIED), to image molecular structures with sub-?ngstr?m precision and exposure times of a few femtoseconds. We apply the method to oxygen and nitrogen molecules, which on strong-field ionization at three mid-infrared wavelengths (1.7, 2.0 and 2.3?μm) emit photoelectrons with a momentum distribution from which we extract diffraction patterns. The long wavelength is essential for achieving atomic-scale spatial resolution, and the wavelength variation is equivalent to taking snapshots at different times. We show that the method has the sensitivity to measure a 0.1?? displacement in the oxygen bond length occurring in a time interval of ~5?fs, which establishes LIED as a promising approach for the imaging of gas-phase molecules with unprecedented spatio-temporal resolution. 相似文献
109.
Becker T Franckenberg S Wickles S Shoemaker CJ Anger AM Armache JP Sieber H Ungewickell C Berninghausen O Daberkow I Karcher A Thomm M Hopfner KP Green R Beckmann R 《Nature》2012,482(7386):501-506
Ribosome-driven protein biosynthesis is comprised of four phases: initiation, elongation, termination and recycling. In bacteria, ribosome recycling requires ribosome recycling factor and elongation factor G, and several structures of bacterial recycling complexes have been determined. In the eukaryotic and archaeal kingdoms, however, recycling involves the ABC-type ATPase ABCE1 and little is known about its structural basis. Here we present cryo-electron microscopy reconstructions of eukaryotic and archaeal ribosome recycling complexes containing ABCE1 and the termination factor paralogue Pelota. These structures reveal the overall binding mode of ABCE1 to be similar to canonical translation factors. Moreover, the iron-sulphur cluster domain of ABCE1 interacts with and stabilizes Pelota in a conformation that reaches towards the peptidyl transferase centre, thus explaining how ABCE1 may stimulate peptide-release activity of canonical termination factors. Using the mechanochemical properties of ABCE1, a conserved mechanism in archaea and eukaryotes is suggested that couples translation termination to recycling, and eventually to re-initiation. 相似文献
110.
Churchill GA Airey DC Allayee H Angel JM Attie AD Beatty J Beavis WD Belknap JK Bennett B Berrettini W Bleich A Bogue M Broman KW Buck KJ Buckler E Burmeister M Chesler EJ Cheverud JM Clapcote S Cook MN Cox RD Crabbe JC Crusio WE Darvasi A Deschepper CF Doerge RW Farber CR Forejt J Gaile D Garlow SJ Geiger H Gershenfeld H Gordon T Gu J Gu W de Haan G Hayes NL Heller C Himmelbauer H Hitzemann R Hunter K Hsu HC Iraqi FA Ivandic B Jacob HJ Jansen RC Jepsen KJ Johnson DK Johnson TE Kempermann G 《Nature genetics》2004,36(11):1133-1137
The goal of the Complex Trait Consortium is to promote the development of resources that can be used to understand, treat and ultimately prevent pervasive human diseases. Existing and proposed mouse resources that are optimized to study the actions of isolated genetic loci on a fixed background are less effective for studying intact polygenic networks and interactions among genes, environments, pathogens and other factors. The Collaborative Cross will provide a common reference panel specifically designed for the integrative analysis of complex systems and will change the way we approach human health and disease. 相似文献