水稻花粉特异性启动子的克隆与表达

利用PCR技术,从水稻幼苗总DNA中扩增并克隆了一段DNA片段。将该片段与报告基因GUS基因融合,构成植物表达载体。经农杆菌转化烟草萌发花粉,共培养约24h后,作瞬时表达检测。结果表明,该克隆片段具有启动子功能。DNA序列分析表明,该克隆片段长度为526bp,含有TATA盒及CAAT盒。与原基因（PSI）启动子序列比较,同源性为52％。部分顺式调控元件相同。     

Single‐index and portfolio models for forecasting value‐at‐risk thresholds

The variance of a portfolio can be forecast using a single index model or the covariance matrix of the portfolio. Using univariate and multivariate conditional volatility models, this paper evaluates the performance of the single index and portfolio models in forecasting value‐at‐risk (VaR) thresholds of a portfolio. Likelihood ratio tests of unconditional coverage, independence and conditional coverage of the VaR forecasts suggest that the single‐index model leads to excessive and often serially dependent violations, while the portfolio model leads to too few violations. The single‐index model also leads to lower daily Basel Accord capital charges. The univariate models which display correct conditional coverage lead to higher capital charges than models which lead to too many violations. Overall, the Basel Accord penalties appear to be too lenient and favour models which have too many violations. Copyright © 2008 John Wiley & Sons, Ltd.     

Redescription of the species of Passalus Fabricius, 1792 (Coleoptera: Passalidae) described by Walter Douglas Hincks (1906–1961) deposited in the Museum of the University of Manchester

ABSTRACT

As part of the effort to study Passalus Fabricius, 1792, we are redescribing and re-illustrating the species of the genus described by Walter Douglas Hincks deposited the Museum of the University of Manchester. Applying characters not yet described for these species as well as morphometric measures we hope to support future taxonomic and phylogenetic studies.     

Tegmina-size variation in a Neotropical cricket with implications on spectral song properties

This study evaluates the relationship between shape and size of tegmen, harp, mirror, and spectral range of calling song frequency of a Neotropical cricket subpopulation (Lerneca inalata beripocone. In addition, we compare intraspecific morphological divergence and calling song properties between individuals from different sites of the Pantanal of Poconé, Mato Grosso, Brazil. Regression analysis showed that the dominant and maximum calling song frequencies were negatively correlated with tegmen size, i.e. frequencies are either lower or higher depending on the corresponding size variation in resonance structures of the forewings. Canonical variable analysis demonstrated marked intraspecific differences in morphometric characters between localities of a L. inalata subpopulation c. 35 km apart (SESC-Pantanal Advanced Research Base and Pouso Alegre Farm, Mato Grosso, Brazil). Lerneca inalata beripocone at SESC had larger forewings than conspecifics from Pouso Alegre Farm. These morphological variations of wing properties related to reproductive behaviours were interpreted as fitness parameters, likely shaped by restricted gene flow during temporal habitat isolation episodes. Such isolation patterns occur in the Pantanal wetlands for several months during the annual hydrological cycle.     

Structure of the insulin receptor ectodomain reveals a folded-over conformation

The insulin receptor is a phylogenetically ancient tyrosine kinase receptor found in organisms as primitive as cnidarians and insects. In higher organisms it is essential for glucose homeostasis, whereas the closely related insulin-like growth factor receptor (IGF-1R) is involved in normal growth and development. The insulin receptor is expressed in two isoforms, IR-A and IR-B; the former also functions as a high-affinity receptor for IGF-II and is implicated, along with IGF-1R, in malignant transformation. Here we present the crystal structure at 3.8 A resolution of the IR-A ectodomain dimer, complexed with four Fabs from the monoclonal antibodies 83-7 and 83-14 (ref. 4), grown in the presence of a fragment of an insulin mimetic peptide. The structure reveals the domain arrangement in the disulphide-linked ectodomain dimer, showing that the insulin receptor adopts a folded-over conformation that places the ligand-binding regions in juxtaposition. This arrangement is very different from previous models. It shows that the two L1 domains are on opposite sides of the dimer, too far apart to allow insulin to bind both L1 domains simultaneously as previously proposed. Instead, the structure implicates the carboxy-terminal surface of the first fibronectin type III domain as the second binding site involved in high-affinity binding.     

Nanog promotes transfer of pluripotency after cell fusion

Through cell fusion, embryonic stem (ES) cells can erase the developmental programming of differentiated cell nuclei and impose pluripotency. Molecules that mediate this conversion should be identifiable in ES cells. One candidate is the variant homeodomain protein Nanog, which has the capacity to entrain undifferentiated ES cell propagation. Here we report that in fusions between ES cells and neural stem (NS) cells, increased levels of Nanog stimulate pluripotent gene activation from the somatic cell genome and enable an up to 200-fold increase in the recovery of hybrid colonies, all of which show ES cell characteristics. Nanog also improves hybrid yield when thymocytes or fibroblasts are fused to ES cells; however, fewer colonies are obtained than from ES x NS cell fusions, consistent with a hierarchical susceptibility to reprogramming among somatic cell types. Notably, for NS x ES cell fusions elevated Nanog enables primary hybrids to develop into ES cell colonies with identical frequency to homotypic ES x ES fusion products. This means that in hybrids, increased Nanog is sufficient for the NS cell epigenome to be reset completely to a state of pluripotency. We conclude that Nanog can orchestrate ES cell machinery to instate pluripotency with an efficiency of up to 100% depending on the differentiation status of the somatic cell.     

新形势下的高校毕业生就业指导与服务

新形势对高校学生就业指导和服务工作提出新的要求 ,本文针对目前高校学生就业指导和服务工作中存在的不足 ,提出必须构建较为完善的集教育、管理、指导、服务为一体的毕业生就业指导与服务体系     

单位矢量在柱、球坐标的变化及微机图示

直接配合图示给出球坐标中单位矢量r0,θ0,β0(当然也包括柱坐标中的单位矢量r0,θ0)随角度θ,β改变时的变化率,并同时给出在微机上成立体画面的操作程序.     

四维变系数对流扩散方程的通用并行数值计算

将Crank-Nicolson隐差分法与分带交替并行方法结合，提出一种绝对稳定的变系数四维（空间三维加时间一维）对流扩散方程的通用并行数值计算模型-Codie4D，可用于模拟一般性的不可压缩流体中的对流扩散过程，利用普遍的MPI库在工作站网络上可并行化实现Codie4D，实验结果表明，Codie4D具有通用性强，无条件稳定，精度高和运行性能好的特点。