甲胺磷席夫碱及其金属配合物的合成与生物活性研究

合成了甲胺磷席夫碱及其Cu(Ⅱ）,Zn(Ⅱ),Ni(Ⅱ)配合物,对它们进行了元素分析和UV,IR测试,对配体进行了MS和1H NMR等结构表征,另外,还对化合物进行了杀灭棉铃虫活性及影响绿豆幼苗细胞存活率实验。     

(Na/ + K+)ATPase has one functioning phosphorylation site per alpha subunit

(Na+ + K+)ATPase contains two different subunits, a catalytic subunit (alpha) and a subunit with uncertain function (beta). The enzyme binds ATP, ouabain and vanadate, and can be phosphorylated by ATP as well as by inorganic phosphate. From the previously reported maximal binding and phosphorylation capacities of 3.5--4.3 nmol P per mg protein (based on Lowry protein determination) and the earlier molecular weight value of approximately 250,000, a molar binding and phosphorylation capacity of 0.87--1.07 mol per mol enzyme was derived. As it is generally agreed that the enzyme molecule contains two alpha subunits or even a multiple of two, it has been suggested that the enzyme operates by means of a so-called "half-of-the-sites mechanism" whereby only of the two alpha subunits can be phosphorylated at any one time. We now present evidence that every alpha subunit can be phosphorylated simultaneously, which rules out the operation of such a mechanism.     

利用虚拟仪器进行测试技术实验教学的研究

对虚拟仪器的发展以及将其引入测试实验教学的必要性和可行性进行了简要叙述,并提出了利用虚拟仪器对理工科院校的测控实验室进行教学改革的实践性和探索性方案。     

西天山那拉提构造带及其找矿前景

那拉提构造带位于天山西段伊犁盆地与南天山之间 ,出露宽度约 2 5 km,由早元古代片岩、片麻岩及混合岩、混合花岗岩组成 ;深层次韧性剪切带及叠加中 -浅层次剪切带发育 ;那拉提构造带南缘断裂明显 ,南侧为古生代浅变质黑色岩系 ;构造带北缘韧 /脆性剪切带宽度大 ,两侧岩石劈理化后易于混淆 ,故界线不明显。那拉提构造带基本地质特征可与邻国中天山对比 ,存在寻找国外类似大型金矿床的条件和可能     

基于SOAP协议的分布式应用研究

介绍基于SOAP协议的分布式应用，讨论如何在分散、异构的Internet环境中实现信息的传递和服务调用。     

Cell-mediated immunity in the liver of mice vaccinated against malaria.

Mice can be protected against several species of lethal malaria infection by vaccination, and their recovery correlates well with increased anti-malarial antibody levels, particularly IgG (ref.2). However, there is also a good correlation between protection by vaccines and priming for delayed-type hypersensitivity in the skin, although there is no obvious explanation for this effect. We now report an apparent relationship between protection and a cell-mediated immune response involving the migration of various types of cell capable of killing malaria parasites in vitro to the liver. We suggest that the effect of vaccination is to bring together parasites, specific antibody and nonspecific cytotoxic cells, and that the liver may be a major site for their interaction.     

Chronic gestational exposure to ethanol causes insulin and IGF resistance and impairs acetylcholine homeostasis in the brain

In fetal alcohol syndrome (FAS), cerebellar hypoplasia is associated with impaired insulin-stimulated survival signaling. This study characterizes ethanol dose-effects on cerebellar development, expression of genes required for insulin and insulin-like growth factor (IGF) signaling, and the upstream mechanisms and downstream consequences of impaired signaling in relation to acetylcholine (ACh) homeostasis. Pregnant Long Evans rats were fed isocaloric liquid diets containing 0%, 2%, 4.5%, 6.5%, or 9.25% ethanol from gestation day 6. Ethanol caused dose-dependent increases in severity of cerebellar hypoplasia, neuronal loss, proliferation of astrocytes and microglia, and DNA damage. Ethanol also reduced insulin, IGF-I, and IGF-II receptor binding, insulin and IGF-I receptor tyrosine kinase activities, ATP, membrane cholesterol, and choline acetyltransferase (ChAT) expression. In vitro studies linked membrane cholesterol depletion to impaired insulin receptor binding and insulin-stimulated ChAT. In conclusion, cerebellar hypoplasia in FAS is mediated by insulin/IGF resistance with attendant impairments in energy production and ACh homeostasis. Received 4 May 2006; received after revision 13 June 2006; accepted 20 June 2006     

The effect of αB-crystallin and Hsp27 on the availability of translation initiation factors in heat-shocked cells

The mechanism of the translational thermotolerance provided by the small heat shock proteins (sHsps) αB-crystallin or Hsp27 is unknown. We show here that Hsp27, but not αB-crystallin, increased the pool of mobile stress granule-associated enhanced green fluorescent protein (EGFP)-eukaryotic translation initiation factor (eIF)4E in heat-shocked cells, as determined by fluorescence recovery after photobleaching. Hsp27 also partially prevented the sharp decrease in the pool of mobile cytoplasmic EGFP-eIF4G. sHsps did not prevent the phosphorylation of eIF2α by a heat shock, but promoted dephosphorylation during recovery. Expression of the C-terminal fragment of GADD34, which causes constitutive dephosphorylation of eIF2α, fully compensated for the stimulatory effect of αB-crystallin on protein synthesis in heat-shocked cells, but only partially for that of Hsp27. Our data show that sHsps do not prevent the inhibition of protein synthesis upon heat shock, but restore translation more rapidly by promoting the dephosphorylation of eIF2α and, in the case of Hsp27, the availability of eIF4E and eIF4G. Received 9 December 2005; received after revision 16 January 2006; accepted 23 January 2006