The ubiquitin-specific protease USP25 interacts with three sarcomeric proteins

The biological functions of the more than one hundred genes coding for deubiquitinating enzymes in the human genome remain mostly unknown. The USP25 gene, located at 21q11.2, encodes three protein isoforms produced by alternative splicing. While two of the isoforms are expressed nearly ubiquituously, the expression of the longer USP25 isoform (USP25m) is restricted to muscular tissues and is upregulated during myogenesis. USP25m interacts with three sarcomeric proteins: actin alpha-1 (ACTA1), filamin C (FLNC), and myosin binding protein C1 (MyBPC1), which are critically involved in muscle differentiation and maintenance, and have been implicated in the pathogenesis of severe myopathies. Biochemical analyses demonstrated that MyBPC1 is a short-lived proteasomal substrate, and its degradation is prevented by over-expression of USP25m but not by other USP25 isoforms. In contrast, ACTA1 and FLNC appear to be stable proteins, indicating that their interaction with USP25m is not related to their turnover rate. Received 7 November 2005; received after revision 7 January 2006; accepted 13 January 2006     

Functional interactions of protein kinase A and C in signalling networks: a recapitulation

On the basis of evidence collected from the literature, we propose a general model by which protein kinase (PK) A and the different PKC isoforms can inversely affect cell growth. Molecular switches, which are able to direct the signal towards antiproliferative or mitogenic pathways, are the different isoforms of Raf and PKC. Conflicting data are also reported and discussed in an attempt to reconcile them. Received 10 November 2005; received after revision 28 December 2005; accepted 3 January 2006     

植物标签技术

传统的正向遗传学主要用于克隆表型或功能已确定的基因。转座子标签突变体可用随机标签法从带有活性转座子的自交后代中筛选得到,或用定向标签法从杂交一代中筛选得到,即用目的基因的隐性突变统合体与具高度活性转座号的统合体杂交,极大多数的FI个体为正常表型,但其中会有极少量的表现隐性性状的转庄子插入突变体。正向基因标签和克隆法在利用异源和低拷贝数品系时尤其有用。采用反向fCR或热不对称交替PCR（TAIL－PCR）且很容易从单拷贝或低拷贝数品系中获得插入于两侧的基因组序列。TAIL－PCR由三轮连续的半巢式PCR组成,所…     

人类的太空未来

人类像乘飞机那样方便地乘太空交通工具巡游外层空间在近几十年的未来可能还实现不了,但在此期间我们将孜孜不倦地为达此目的而攻研多项重大课题：倾全力设计新型发射运载工具、建立国际太空空间站、寻索太空能……l993年,美国国会通过一项表决,拯救了国际太空空间站项目的立项,这是发生在人类征服太空史上的一件重大事件,此项意义特殊重大的关键可能将要深深影响着人类的未来。事实已经证明,建造国际太空空间站实际上对西方国家探索日益增长的大量科技领域新知提供了促激动力。若人类不希望成为仅立足于地球上而面临人口日益拥挤的被…     

BEM Analysis of Wave Propagation in a Water-Filled Borehole in an Anisotropic Solid

This paper describes a time-domain boundary element method developed to analyze the interactions of acoustic and elastic waves near the interfaces between water and an anisotropic elastic solid. Two models are analyzed with one being the interface between two half spaces of fluid and solid and the other being a fluid region sandwiched by half space domains of anisotropic elastic solids. Both monopole and dipole point sources are used to generate an initial pressure wave in the fluid. Some snapshots of the transient wave behavior near the fluid-solid interfaces are given. The effect of the anisotropy in the solid on the pressure waveforms in the fluid is discussed. The numerical results allow detailed arrival identification and interpretation of acoustic and elastic waves propagating along the fluid-solid interfaces.     

基于EXCEL和MATLAB的矩形薄片热传导计算与仿真研究

对二维导热问题进行了离散化研究,推导出了节点差分方程,以矩形薄片为例,用MS.Excel对节点温度进行了计算,用MATLAB对计算结果进行了仿真,得到了薄片的温度分布仿真图.     

关于Quarter可层化空间

对第二纲的仿紧Quarter可层化空间进行了研究,证明了这类空间含有σ闭离散的稠密子集. 同时给出了实数集的子集是消去拓扑半群,但是不是Quarter可层化空间.     

Spectrum characteristics and pump wavelengths of Nd3+-doped double-clad fiber

Conclusion The optimum wavelength of Nd³⁺-doped double-clad fiber laser or amplifier made in China is near 830 nm. The efficiency of Nd³⁺-doped double-clad fiber devices pumped by 820-nm laser is low due to ESA. In order to increase the photochemical stability of the outer cladding, the material of the outer cladding must be advanced.     

基于可控多扫描使能信号的片上系统TR-TC联合测试成本模型

基于片上系统的扫描链结构,针对全速测试研究了多扫描使能（SE）信号的可测性设计,并建立了新颖的测试资源 覆盖率（TR-TC）联合测试成本线性规划数学模型.研究结果表明,该模型不仅可以高效控制全速测试的测试资源消耗以及可测性设计复杂度,而且还可以确立SE信号数量的最优上限,进而避免了以盲目提升SE信号数量来提高转换故障覆盖率的纯理论方式,使面向片上系统全速测试的多SE信号可测性设计方法有一个可靠的目标控制值.     

世界优秀男子110 m栏运动员成绩变化分析

采用文献资料调查、数理统计等科研方法, 对1997年世界田径锦标赛男子110 m栏前 7 名运动员的成绩进行相关分析, 揭示当今男子110 m栏全程速度变化的规律和特点, 并对近 4 年来世界优秀男子 110 m栏运动员与我国男子110 m栏运动员的成绩进行对比分析, 希望能对我国男子110 m栏的训练提供一些新的参考和启示.