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31.
Of 52 serum samples from infertile women tested against isolated porcine oocytes by immunofluorescence, 8 produced intense reactions in zonae pellucidae, while only one of 52 sera from control subjects showed the reaction. Autoantibodies to the zona may be present in these women. 相似文献
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Expression of functional acetylcholine receptor from cloned cDNAs 总被引:40,自引:0,他引:40
M Mishina T Kurosaki T Tobimatsu Y Morimoto M Noda T Yamamoto M Terao J Lindstrom T Takahashi M Kuno 《Nature》1984,307(5952):604-608
The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the alpha-subunit is indispensable for alpha-bungarotoxin binding activity. 相似文献
35.
T. Mori T. Nishimoto M. Kitagawa Y. Noda T. Nishimura T. Oikawa 《Cellular and molecular life sciences : CMLS》1978,34(6):797-799
Summary Of 52 serum samples from infertile women tested against isolated porcine oocytes by immunofluorescence, 8 produced intense reactions in zonae pellucidae, while only one of 52 sera from control subjects showed the reaction. Autoantibodies to the zona may be present in these women.This work was supported in part by a grant from WHO Task Force on Immunological Methods for Fertility Regulation Project No. 76464. 相似文献
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Dioxin receptor is a ligand-dependent E3 ubiquitin ligase 总被引:2,自引:0,他引:2
Ohtake F Baba A Takada I Okada M Iwasaki K Miki H Takahashi S Kouzmenko A Nohara K Chiba T Fujii-Kuriyama Y Kato S 《Nature》2007,446(7135):562-566
38.
In viruses, as in eukaryotes, elaborate mechanisms have evolved to protect the genome and to ensure its timely replication and reliable transmission to progeny. Influenza A viruses are enveloped, spherical or filamentous structures, ranging from 80 to 120 nm in diameter. Inside each envelope is a viral genome consisting of eight single-stranded negative-sense RNA segments of 890 to 2,341 nucleotides each. These segments are associated with nucleoprotein and three polymerase subunits, designated PA, PB1 and PB2; the resultant ribonucleoprotein complexes (RNPs) resemble a twisted rod (10-15 nm in width and 30-120 nm in length) that is folded back and coiled on itself. Late in viral infection, newly synthesized RNPs are transported from the nucleus to the plasma membrane, where they are incorporated into progeny virions capable of infecting other cells. Here we show, by transmission electron microscopy of serially sectioned virions, that the RNPs of influenza A virus are organized in a distinct pattern (seven segments of different lengths surrounding a central segment). The individual RNPs are suspended from the interior of the viral envelope at the distal end of the budding virion and are oriented perpendicular to the budding tip. This finding argues against random incorporation of RNPs into virions, supporting instead a model in which each segment contains specific incorporation signals that enable the RNPs to be recruited and packaged as a complete set. A selective mechanism of RNP incorporation into virions and the unique organization of the eight RNP segments may be crucial to maintaining the integrity of the viral genome during repeated cycles of replication. 相似文献
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Atg1 family kinases in autophagy initiation 总被引:1,自引:1,他引:0
40.
Maiko Noda Alexei V. Abramov Galina Baranova Gennady Baryshinikov Hideo Ichikawa 《Journal of Natural History》2016,50(11-12):771-786
To test postglacial population expansion in small arboreal mammals dependent on boreal and subboreal forests, we used complete mitochondrial cytochrome b gene sequences (1140 bases) to investigate the phylogeography of the Eurasian red squirrel (Sciurus vulgaris orientis) on the Hokkaido Island of Japan. This subspecies is common in the boreal and subboreal forests of Hokkaido. We examined 61 specimens from 27 localities in Hokkaido. Phylogenetic relationships among 29 haplotypes found in the Hokkaido populations were not associated with geographic distribution of sampling localities. There were four mitochondrial DNA phylogroups. Phylogeographic analyses support sudden expansion of S. vulgaris orientis from restricted refugium in the southern part of Hokkaido during the last glacial period. The phylogeographic structure of this subspecies directly reflects the boreal and subboreal forest dynamics occurring in Hokkaido. 相似文献