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Proteins are typically categorized into protein families based on their domain organization. Yet, evolutionarily unrelated proteins can also be grouped together according to their common functional roles. Sequestering proteins constitute one such functional class, acting as macromolecular buffers and serving as an intracellular reservoir ready to release large quantities of bound proteins or other molecules upon appropriate stimulation. Another functional protein class comprises effector proteins, which constitute essential components of many intracellular signal transduction pathways. For instance, effectors of small GTP-hydrolases are activated upon binding a GTP-bound GTPase and thereupon participate in downstream interactions. Here we describe a member of the IQGAP family of scaffolding proteins, DGAP1 from Dictyostelium, which unifies the roles of an effector and a sequestrator in regard to the small GTPase Rac1. Unlike classical effectors, which bind their activators transiently leading to short-lived signaling complexes, interaction between DGAP1 and Rac1-GTP is stable and induces formation of a complex with actin-bundling proteins cortexillins at the back end of the cell. An oppositely localized Rac1 effector, the Scar/WAVE complex, promotes actin polymerization at the cell front. Competition between DGAP1 and Scar/WAVE for the common activator Rac1-GTP might provide the basis for the oscillatory re-polarization typically seen in randomly migrating Dictyostelium cells. We discuss the consequences of the dual roles exerted by DGAP1 and Rac1 in the regulation of cell motility and polarity, and propose that similar signaling mechanisms may be of general importance in regulating spatiotemporal dynamics of the actin cytoskeleton by small GTPases.  相似文献   
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Zusammenfassung Supernatanten aus Kulturen überempfindlicher Lymphozyten und dem spezifischen Antigen mit MIF-Aktivität können die Entstehung der Pseudopodien normaler Makrophagen verhindern und zeigen nach längerem Kultivieren zuweilen toxische Eigenschaften.  相似文献   
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This article emphasizes the role of projects and project management in assuring socially responsible acting of contemporary business systems, presenting some practical applications in the field of tourism. With arising number of projects in business systems and their growing strategic importance, the role of project management has radically been changing for the last two decades. Managing organizations, as a central topic among seven crucial topics in ISO 26000 on social responsibility, is strongly associated with managing projects. Projects have important influence on the established as well as on establishing ongoing operations and are important tools in assuring business systems’ strategic development, by exposing interdependences and supporting the requisitely holistic approach. This article tries to fill the gap we detected in literature on social responsibility where projects and project management seem not to have a proper role. Four different links and interdependencies between project management and systems theory (which ISO 26000 reinforces) are exposed: (1) systemic treatment of project management, (2) systematical treatment of projects as holistic works, (3) systemic treatment of projects as parts of BSs’ strategies, (4) systemic treatment of projects throughout their entire life cycle.  相似文献   
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A stochastic service system of finite size M is comprised of identical service facilities,including or not a waiting queue,which simultaneously treats N custome...  相似文献   
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Choanoflagellates are the closest known relatives of metazoans. To discover potential molecular mechanisms underlying the evolution of metazoan multicellularity, we sequenced and analysed the genome of the unicellular choanoflagellate Monosiga brevicollis. The genome contains approximately 9,200 intron-rich genes, including a number that encode cell adhesion and signalling protein domains that are otherwise restricted to metazoans. Here we show that the physical linkages among protein domains often differ between M. brevicollis and metazoans, suggesting that abundant domain shuffling followed the separation of the choanoflagellate and metazoan lineages. The completion of the M. brevicollis genome allows us to reconstruct with increasing resolution the genomic changes that accompanied the origin of metazoans.  相似文献   
38.
The vector alignments of asteroid spins by thermal torques   总被引:1,自引:0,他引:1  
Vokrouhlický D  Nesvorný D  Bottke WF 《Nature》2003,425(6954):147-151
Collisions have been thought to be the dominant process altering asteroid rotations, but recent observations of the Koronis family of asteroids suggest that this may be incorrect. This group of asteroids was formed in a catastrophic collision several billion years ago; in the intervening period their rotational axes should have become nearly random because of subsequent collisions, with spin rates that follow a maxwellian distribution. What is seen, however, is that the observed family members with prograde spins have nearly identical periods (7.5-9.5 h) and obliquities between 42 and 50 degrees, while those with retrograde spins have obliquities between 154 and 169 degrees with periods either <5 h or >13 h. Here we show that these non-random orientations and spin rates can be explained by 'thermal torques' (arising from differential solar heating), which modify the spin states over time. In some cases, the asteroids become trapped in spin-orbit resonances. Our results suggest that thermal torques may be more important than collisions in changing the spin states (and possibly shapes) of asteroids with diameters <40 km.  相似文献   
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Volkov I  Banavar JR  Hubbell SP  Maritan A 《Nature》2007,450(7166):45-49
A formidable many-body problem in ecology is to understand the complex of factors controlling patterns of relative species abundance (RSA) in communities of interacting species. Unlike many problems in physics, the nature of the interactions in ecological communities is not completely known. Although most contemporary theories in ecology start with the basic premise that species interact, here we show that a theory in which all interspecific interactions are turned off leads to analytical results that are in agreement with RSA data from tropical forests and coral reefs. The assumption of non-interacting species leads to a sampling theory for the RSA that yields a simple approximation at large scales to the exact theory. Our results show that one can make significant theoretical progress in ecology by assuming that the effective interactions among species are weak in the stationary states in species-rich communities such as tropical forests and coral reefs.  相似文献   
40.
Summary DPP IV activity was localized in the nerve fascicles of cat glabrous skin at light and electron microscope levels. The observation that the DPP IV end product was restricted to the axon-Schwann cell interface suggests that this enzyme may be involved in the interactions between unmyelinated axons and their Schwann cells.17 November 1986  相似文献   
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