全文获取类型
收费全文 | 19123篇 |
免费 | 48篇 |
国内免费 | 41篇 |
专业分类
系统科学 | 116篇 |
丛书文集 | 263篇 |
教育与普及 | 30篇 |
理论与方法论 | 117篇 |
现状及发展 | 9400篇 |
研究方法 | 855篇 |
综合类 | 8215篇 |
自然研究 | 216篇 |
出版年
2013年 | 110篇 |
2012年 | 271篇 |
2011年 | 497篇 |
2010年 | 122篇 |
2008年 | 300篇 |
2007年 | 317篇 |
2006年 | 363篇 |
2005年 | 355篇 |
2004年 | 454篇 |
2003年 | 312篇 |
2002年 | 317篇 |
2001年 | 514篇 |
2000年 | 464篇 |
1999年 | 344篇 |
1992年 | 305篇 |
1991年 | 236篇 |
1990年 | 261篇 |
1989年 | 243篇 |
1988年 | 214篇 |
1987年 | 258篇 |
1986年 | 290篇 |
1985年 | 355篇 |
1984年 | 250篇 |
1983年 | 235篇 |
1982年 | 231篇 |
1981年 | 231篇 |
1980年 | 251篇 |
1979年 | 631篇 |
1978年 | 529篇 |
1977年 | 510篇 |
1976年 | 361篇 |
1975年 | 433篇 |
1974年 | 644篇 |
1973年 | 518篇 |
1972年 | 552篇 |
1971年 | 607篇 |
1970年 | 765篇 |
1969年 | 605篇 |
1968年 | 592篇 |
1967年 | 608篇 |
1966年 | 492篇 |
1965年 | 416篇 |
1964年 | 134篇 |
1959年 | 197篇 |
1958年 | 337篇 |
1957年 | 253篇 |
1956年 | 190篇 |
1955年 | 196篇 |
1954年 | 196篇 |
1948年 | 130篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
51.
52.
53.
54.
J E Landegent N Jansen in de Wal G J van Ommen F Baas J J de Vijlder P van Duijn M Van der Ploeg 《Nature》1985,317(6033):175-177
During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AAF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8. A mixture of cosmid cHT2-derived subclones of the 3' part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated. 相似文献
55.
56.
Neurotransmission from mossy fibre terminals onto cerebellar granule cells is almost certainly mediated by L-glutamate. By taking advantage of the small soma size, limited number of processes and short dendrite length of granule cells, we have obtained high-resolution recordings of spontaneous miniature excitatory postsynaptic currents (m.e.p.s.cs) and evoked currents in thin cerebellar slices. Miniature currents have a similar time-course and pharmacology to evoked currents and consist of an exceptionally fast non-NMDA (N-methyl-D-aspartate) component (measured rise-time, 200 microseconds; estimated pre-filtered rise-time less than 100 microseconds; decay time constant, tau = 1.0 ms), followed by 50 pS NMDA channel openings that are directly resolvable. We could find no evidence for the recent proposal that miniature currents in granule cells are mediated solely by NMDA channels with a novel time course. The non-NMDA receptor component of m.e.p.s.cs has a skewed amplitude distribution, which suggests potential complications for quantal analysis. The difference in time course between the m.e.p.s.cs reported here and other synaptic currents in the brain could reflect differences in synaptic function or electrotonic filtering; the relative contribution of these possibilities has yet to be established. 相似文献
57.
The fecundity of the blood-feeding insect,Rhodnius prolixus, was observed to increase in successive periods of egg production, each period being triggered by a single large blood meal. As previously published, the fecundity of mated animals was significantly higher than that of unmated animals for the first period of egg production. For a second period of egg production, fecundity increased significantly in both mated and unmated animals. By the fourth period, fecundity had returned to first-feed values for mated animals, but remained high for unmated animals, and the fecundity of mated and unmated animals was not significantly different. Thus, during successive periods of egg production, the processes which maintain fecundity of unmated animals below that of mated animals are overcome. 相似文献
58.
P. Levy G. Cherqui A. Robert D. Wicek J. Picard 《Cellular and molecular life sciences : CMLS》1989,45(6):588-591
Summary During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4-phorbol 12-myristate, 13-acetate and 1,2-dioctanoyl-glycerol inhibited glycosaminoglycan sulfation. By contrast, 4-phorbol 12, 13 didecanoate was ineffective.These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses. 相似文献
59.
J. P. Barthélemy 《Journal of Classification》1988,5(1):85-87
The theorem of the paper Aggregation of Equivalence Relations, by Fishburn and Rubinstein, states a result already known. This theorem improves a result from Mirkin (1975) and appears as a corollary occurring in Leclerc (1984).
Resume L'unique théorème de l'article Aggregation of Equivalence Relations de Fishburn et Rubinstein est déja connu. Il améliore, en fait, un résultat de Mirkin (1975) et apparait en tant que corollaire dans Leclerc (1984).相似文献
60.
G. C. Hewitt 《Cellular and molecular life sciences : CMLS》1988,44(4):297-303
Summary Public suspicion of science stems from science's challenging of perceptions and myths about reality, and a public fear of new technology. The result is a susceptibility to pseudoscience. In claiming that creation science is as valid as evolution the creationists misquote scientists and seek to spread their own scientific myths concerning a young age for the earth, an act of creation based on a particular literalist interpretation of the Christian Bible and a single worldwide flood. They use methods of debate and politics, rather than scientific research. A selection of their arguments is examined and the nature of the evidence for evolution is discussed. Problems with the creation science model are noted. In the myth of the hundredth monkey phenomenon, original research is misquoted to denigrate scientific research and support sentimental ideas of paranormal events. The misuse of science is seen as damaging to society because it reduces the effective gathering and application of scientific information. However, pseudoscience provides a valuable guide to gaps in public scientific education. 相似文献