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785.
L. Máródi J. Jezerniczky S. Csorba L. Karmazsin P. C. J. Leijh R. van Furth 《Cellular and molecular life sciences : CMLS》1984,40(12):1407-1410
Summary Chemotactic responsiveness and random movement of cord-blood granulocytes were studied with a modified Boyden's method. Cord-blood granulocytes were less active chemotactically than granulocytes from healthy children and adults, whereas the random filter movement of the cells from all three sources was about the same. In cord sera, concentrations of cell directed chemotaxis inhibitors were equal to those in sera from other age groups. Compared with the situation in healthy children and adults, the generation of chemotactic factors in cord-blood sera was impaired. This impairment was not related to an increased activity of chemotactic factor inactivators. Measurement of the cyclic nucleotide levels in granulocytes from cord-blood and from children belonging to various age groups revealed that the cord granulocytes have significantly lower concentrations of cAMP and cGMP, which could have been responsible for the decreased chemotactic responsiveness. 相似文献
786.
Chiral selection in poly(C)-directed synthesis of oligo(G) 总被引:3,自引:0,他引:3
G F Joyce G M Visser C A van Boeckel J H van Boom L E Orgel J van Westrenen 《Nature》1984,310(5978):602-604
Theories of the origin of optical asymmetry in living systems place fundamental importance on the amplification of optical asymmetry by an autocatalytic process. The replication of a polynucleotide is one obvious choice for such an autocatalytic growth mechanism. If an optically homogeneous polynucleotide could replicate by directing the polymerization of monomers of the same handedness, while excluding monomers of the opposite handedness, its chiral descendants would come to dominate what was once an achiral environment. Recently, two highly efficient template-directed reaction systems have been developed for the oligomerization of activated guanosine mononucleotides (Fig. 1) on a poly(C) template. The synthesis of L-guanosine 5'-mononucleotide makes it possible to study chiral selection in these systems. We report here that poly(C)-directed oligomerization of activated guanosine mononucleotides proceeds readily if the monomers are of the same optical handedness as the template, and is indeed far less efficient if the monomers are of the opposite handedness. However, in template-directed reactions with a racemic mixture, monomers of the opposite handedness to the template are incorporated as chain terminators at the 2'(3') end of the products. This inhibition raises an important problem for many theories of the origin of life. 相似文献
787.
Summary Two h of nerve stimulation at 10 Hz or of elevated spontaneous release in hypertonic solution increased the size of miniature end-plate potentials (m.e.p.p.'s) and currents at the frog neuromuscular junction, probably by increasing the amount of acetylcholine in a quantum. Increases in quantal size may modulate synaptic transmission.Acknowledgments. Supported by grant 10320 from the NINCDS and by a grant from the MDA. We are grateful to Dr I.S. Cohen for participating in some of the experiments and for helpful criticism, and to Drs C. Clausen and G. Baldo for help with the computer. Some of the results were presented at a meeting of the Physiological Society. 相似文献
788.
G A Schuiling N Pols-Valkhof G C van der Schaaf-Verdonk F H de Jong T R Koiter 《Experientia》1987,43(8):895-898
The mid-pregnancy rescue of corpora lutea can be mimicked in the pseudopregnant rat by induction of decidual tissue in the uterus: in such rats, around day 10, there is neither luteolysis, nor resumption of follicle-development or increase of the pituitary responsiveness to LHRH. The results suggest that the mid-pregnancy rescue of corpora lutea is caused by a maternal factor. 相似文献
789.
T. R. Koiter G. C. J. van der Schaaf-Verdonk S. Broers N. Pols-Valkhof G. A. Schuiling 《Cellular and molecular life sciences : CMLS》1986,42(3):304-307
Summary In ovariectomized rats the desensitization of the LH cells in vivo, which develops during constant rate infusion of LHRH, 1) does not depend on a concomitant depletion of the pituitary LH stores, 2) proceeds normally when the hypothalamo-pituitary connection has been severed and 3) is a process in which LH itself is not involved. 相似文献
790.
Summary Adequate moisture was transported by an electrical potential gradient of 1 V/cm of the correct polarity to maintain the turgid state of broad-bean plants subjected to severe stress conditions.Acknowledgments. A.P. is grateful to the University of stellenbosch for the financial aid and the privilege of conducting research on their premises. 相似文献