Identification of lymphocyte subpopulations by simultaneous E-rosette formation and unspecific acid esterase staining.

E-rosettes of human peripheral blood lymphocytes were stained by an unspecific acid esterase stain; 74.5% of the rosette forming cells were esterase positive, while only 30% of the non-rosette forming cells showed a reaction product.     

The effects of vitamin A nutritional status on microsomal lipid peroxidation and alpha-tocopherol level in rat liver

In vitamin A-deficient rats, liver glutathione peroxidase activity was decreased, alpha-tocopherol content was strongly enhanced, but microsomal liquid peroxidation remained unchanged.     

On the structure of pterobilin,the blue pigment ofPieris brassicae

Zusammenfassung Aus dem Tegument von Raupen des Kohlweisslings (Pieris brassicae) wird das bereits früher beschriebene Pterobilin isoliert, das auf Grund seiner Abbauprodukte und der chromatographischen und spektralen Eigenschaften als Biliverdin-IX identifiziert wird.     

Stereological measurements of atrial ultrastructures in the guinea-pig

Zusammenfassung Mit morphometrischen Methoden wurde beim Meerschweinchen eine quantitative Analyse von Volumen und Oberflächenareal der Ultrastrukturkomponenten der Herzvorhof-Muskulatur durchgeführt, die mit dem Erregungs-Kontraktions-Kopplungsvorgang und der Erschlaffung verknüpft sind.     

Genetic variation in DLG5 is associated with inflammatory bowel disease

Crohn disease and ulcerative colitis are two subphenotypes of inflammatory bowel disease (IBD), a complex disorder resulting from gene-environment interaction. We refined our previously defined linkage region for IBD on chromosome 10q23 and used positional cloning to identify genetic variants in DLG5 associated with IBD. DLG5 encodes a scaffolding protein involved in the maintenance of epithelial integrity. We identified two distinct haplotypes with a replicable distortion in transmission (P = 0.000023 and P = 0.004 for association with IBD, P = 0.00012 and P = 0.04 for association with Crohn disease). One of the risk-associated DLG5 haplotypes is distinguished from the common haplotype by a nonsynonymous single-nucleotide polymorphism 113G-->A, resulting in the amino acid substitution R30Q in the DUF622 domain of DLG5. This mutation probably impedes scaffolding of DLG5. We stratified the study sample according to the presence of risk-associated CARD15 variants to study potential gene-gene interaction. We found a significant difference in association of the 113A DLG5 variant with Crohn disease in affected individuals carrying the risk-associated CARD15 alleles versus those carrying non-risk-associated CARD15 alleles. This is suggestive of a complex pattern of gene-gene interaction between DLG5 and CARD15, reflecting the complex nature of polygenic diseases. Further functional studies will evaluate the biological significance of DLG5 variants.     

An olivine-free mantle source of Hawaiian shield basalts

More than 50 per cent of the Earth's upper mantle consists of olivine and it is generally thought that mantle-derived melts are generated in equilibrium with this mineral. Here, however, we show that the unusually high nickel and silicon contents of most parental Hawaiian magmas are inconsistent with a deep olivine-bearing source, because this mineral together with pyroxene buffers both nickel and silicon at lower levels. This can be resolved if the olivine of the mantle peridotite is consumed by reaction with melts derived from recycled oceanic crust, to form a secondary pyroxenitic source. Our modelling shows that more than half of Hawaiian magmas formed during the past 1 Myr came from this source. In addition, we estimate that the proportion of recycled (oceanic) crust varies from 30 per cent near the plume centre to insignificant levels at the plume edge. These results are also consistent with volcano volumes, magma volume flux and seismological observations.