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91.
92.
F Awouters C J Niemegeers J Van den Berk J M Van Neuten F M Lenaerts M Borgers K H Schellekens A Broeckaert J De Cree P A Janssen 《Experientia》1977,33(12):1657-1659
Oxatomide is a new potent inhibitor of anaphylactic and allergic reactions. After oral administration, the compound both inhibits the release of endogenous histamine and prevents the effects of exogensous histamine, at comparable doses. The combination of these effects appears to be the basis of the effectiveness of oxatomide in allergic reactions and may lead to clinical application different from classical antihistaminics and from cromoglycate. 相似文献
93.
94.
Prokaryotic origin of the actin cytoskeleton. 总被引:47,自引:0,他引:47
It was thought until recently that bacteria lack the actin or tubulin filament networks that organize eukaryotic cytoplasm. However, we show here that the bacterial MreB protein assembles into filaments with a subunit repeat similar to that of F-actin-the physiological polymer of eukaryotic actin. By elucidating the MreB crystal structure we demonstrate that MreB and actin are very similar in three dimensions. Moreover, the crystals contain protofilaments, allowing visualization of actin-like strands at atomic resolution. The structure of the MreB protofilament is in remarkably good agreement with the model for F-actin, showing that the proteins assemble in identical orientations. The actin-like properties of MreB explain the finding that MreB forms large fibrous spirals under the cell membrane of rod-shaped cells, where they are involved in cell-shape determination. Thus, prokaryotes are now known to possess homologues both of tubulin, namely FtsZ, and of actin. 相似文献
95.
J van den Berg A van Ooyen N Mantei A Schamb?ck G Grosveld R A Flavell C Weissmann 《Nature》1978,276(5683):37-44
Cloned beta-globin genes of both mouse and rabbit each contain a large and a small intervening sequence (intron) of about equal length at precisely the same positions relative to the coding sequence. The homologous introns show some sequence similarity, particularly at the junctions with the coding sequence. They most probably arose from a common ancestral sequence and diverged substantially during evolution. 相似文献
96.
J. van den Bercken S. Broekhuizen J. Ringelberg H. H. W. Velthuis 《Cellular and molecular life sciences : CMLS》1967,23(1):44-45
Zusammenfassung Die Versuche vonPapi undPardi
1–6 wurden mitTalitrus saltator wiederholt. Auch unter völligem Lichtabschluss zeigten die Tiere ein noch ähnlich orientiertes Verhalten. Ausschaltung des erdmagnetischen Feldes bis auf 50 konnte die Orientierung im Dunkeln nicht aufheben. 相似文献
97.
98.
R. J. Hamilton W. J. A. Van den Heuvel E. C. Horning 《Cellular and molecular life sciences : CMLS》1964,20(10):568-568
Résumé Les auteurs ont extrait un stérol d'une méduse des eaux portugaises (Physalia physalis) et l'ont identifié par chromatographie en phase gazeuse comme étant du cholestérol. 相似文献
99.
Protein-tyrosine phosphatases (PTPs) are vital for regulating tryosine phosphorylation in many processes, including growth and differentiation. The regulation of receptor-like PTP (RPTP) activity remains poorly understood, but based on the crystal structure of RPTPalpha domain 1 we have proposed that dimerization can negatively regulate activity, through the interaction of an inhibitory 'wedge' on one monomer with the catalytic cleft of domain 1 in the other monomer. Here we show that dimerization inhibits the activity of a full-length RPTP in vivo. We generated stable disulphide-bonded full-length RPTPalpha homodimers by expressing mutants with single cysteines at different positions in the ectodomain juxtamembrane region. Expression of wild-type RPTPalpha and Phe135Cys and Thr141Cys mutants in RPTPalpha-null mouse embryo cells increased dephosphorylation and activity of Tyr 529 in the protein tyrosine kinase c-Src; in contrast, expression of a Pro137Cys mutant did not. Mutation of Pro 210/211 to leucine in the inhibitory wedge of the Pro137Cys mutant restored its ability to activate c-Src, indicating that dimerization may inhibit full-length RPTPalpha activity in a manner stereochemically consistent with RPTPalpha crystal structures. Our results suggest that RPTPalpha activity can in principle be negatively regulated by dimerization in vivo. 相似文献
100.