Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

竞技游泳出发台出发过程中各影响因素的量化分析

运用物理学知识建立了游泳出发过程的数学模型。采用优化算法求得出发过程的最佳参数，并对出发台出发过程中的各影响因素进行了灵敏度分析，确定了各影响因素的主次关系，为制定科学的训练计划提供了依据。     

不完全投影据图像重建的最小交叉熵算法

提出图像重建的最小交叉熵算法,详细推导了算法的有关公式,并给出程序实现框图.计算机模拟结果表明:最小交叉熵算法的性能优于最大熵算法.     

电弧放电法快速合成金刚石薄膜

本文用电弧放电等离子体ＣＶＤ快速生长金刚石薄膜。用拉曼散射，Ｘ射线衍射、电子显微镜等方法进行了结构表征。文中所论述的恒定的基板温度对沉积金刚石薄膜是非常重要的。     

可调谐的同轴腔滤波器

本文介绍一种带宽为5～8MHz,调谐范围在350～510MHz的同轴腔带通滤波器。详细地介绍了腔间孔耦合理论。给出消除耦合环自感影响的方法及减弱电耦合的途径,並给出了实测结果与理论计算的比较。     

Banach空间一类常微分方程广义弱解的存在性

在弱完备的实Ｂａｎａｃｈ空间中，考虑如下的Ｃａｕｃｈｙ问题：ｘ′（ｔ）＝ｆｏ（ｔ，ｘ（ｔ）），ｘ（０）＝ｘｏ，（ｃｐ）其中ｆｏ＝ｆ＋ｇ，ｆ和ｇ满足不同的弱非紧型条件．证明一个满足不同弱非紧型条件的算子间的关系式和（ｃｐ）的广义弱解的存在定理．利用这些结果，得到一个定理，该定理的特例是［１］中定理５１１的推广     

光学式水蒸汽湿度测量仪的研制

介绍一种光学式水蒸汽湿度测量仪的工作原理、结构与性能特点．本仪器可用于测量水蒸汽的湿度和粒径；并可推广应用于悬浮介质中微粒直径和浓度的测量．     

红树林凋落叶自然分解过程中土壤微生物的数量动态

用人工模拟凋落叶方法.研究了红树林(秋茄Kandelia candel)凋落叶自然分解过程中土壤微生物数量的变化,结果表明:凋落叶有利于土壤异养微生物的生长,繁殖,使其数量明显增多;从而加速了凋落叶的分解作用,引起凋落叶分解的异养微生物,从数量看,细菌占绝对优势,它是落叶分解起先蜂和主导作用的微生物类群;丝状真菌也有不可忽视的作用;放线菌似乎被抑制而减少。     

小幅振动旋成体跨音速绕流的数值分析

假设绕小幅振动旋成体所导致的物理量的变化为平均定常扰动量的高阶小量,那么将物理量(速度、密度及压力)摄动展开后代入Euler方程,便导出了前两阶摄动量的控制方程和相应的边界条件,用TVD格式进行数值求解,得到了平均定常跨音速流(零阶)和基频(一阶)非定常分量的数值解。     

世纪末中国文学病态论

本文以宏观和俯瞰的视角,对90年代以来中国当代文学发展状况作了一次回观和审视．对文学发展中呈现出的诸钏病态提出了尖锐的、中肯的批评,如文学对“道”的放弃,文学家中缺乏“思想者”,文学创作对“底层”缺少应有的人文关注以及女性文学内质的退化弱化等等,并对其产生的恶果及原因作了一定程度的分析．指出文学家在面临危机与挑战时应有的姿态与选择,指出文学的生存发展除了受到社会外力的作用外,人的因素是第一位的。