浅孔密集钻注浆及有关参数的确定

本文阐述采用浅孔密集钻注浆时,钻孔的布置和设计,注浆材料的粒度分级和水土比的简易测定方法,以及泥浆输送中计算水力坡度、临界流速和倍线时有关参数的确定。     

基于静电场原理的图像分辨率增强算法

根据静电场的平衡原理，提出了图像分辨率的增强算法，其内插函数随相邻像素的电势能差和所处的能量带可以自动调节，从而实现了图像的自适应放大．在对彩色图像放大中，算法模型考虑了各个色彩分量对图像插值产生的影响，克服了常规方法对整幅图像无区别，以及采用同一种内插函数进行处理时所带来的边缘模糊效应和阶梯形失真，同时又消除了对各个颜色分量分别处理所产生的颜色失真现象．试验的各个性能指标表明，该算法与传统方法相比，插值后得到的图像不仅在图像质量上有明显的改善，而且能保持清晰的边缘和轮廓信息，因此可在遥感图像、医学系统、公安系统及一些图像处理的软件中得到应用．     

隔振前后振动筛对基础动负荷的理论研究

根据单自由度系统的振动理论,详细推导了振动筛对基础动负荷的计算公式;采用两个自由度系统力学模型,利用隔振理论,计算了带隔振台的振动筛对基础的动负荷,计算了采用隔振台和没有采用隔振台两种情况下对基础动负荷的比值,分析了隔振效果;为大型振动筛设计提供理论依据。     

非线性反问题求解的参数微分正则化方法

讨论了非线性反问题的求解问题,将具有大范围收敛特性的同伦方法引入到非线性反问题的求解之中,籍此克服非线性反问题常规求解过程中局部收敛的缺陷;结合吉洪诺夫正则化方法,以解决计算Frechet导数时病态的问题.在此基础上,提出了一种用于求解非线性反问题的参数微分正则化方法,给出其构造过程,并且证明了参数微分正则化方法解的存在性和收敛性.     

营造讲真话的德育环境

德育是素质教育的核心,但是长期以来德育并没能有效发挥其育人的作用。根本原因就在于德育脱离了对学生思想实际的把握。要改变这一现状,必须转变德育理念、改变灌输式的德育方法,从鼓励学生讲真话着手,夯实德育的基础。     

机器人奇异形位分析及协调控制方法

把6R机器人奇异形位分为边界奇异、内部奇异、结构边界奇异和腕部奇异，分析了这些奇异形位的形成条件，并对每一种奇异进行了计算机仿真。在深入分析奇异边界构成的基础上，给出了内部奇异直观的计算机图形仿真结果，克服了以往笼统而欠实用描述方法的不足。通过分析J^-1(J为Jacobian矩阵）提出了采用协调控制法回避机械手奇异形位的理论及在线实时控制方法。     

基于Voronoi图的机器人复杂装配新策略

提出了一种基于Voronoi图技术的机器人复杂装配的新策略；在机器人装配研究中引入形象思维方式，即集成Voronoi图技术；装配知识的表示采用Voronoi图方式，通过Voronoi分析对机器人复杂装配过程进行有效规划，从而形成机器人关于装配过程的所有过程；机器人于通过力觉和力控制来感知装配件之间的精确物理关系，并调整装配件的位姿，实施基于Voronoi图知识的机器人装配，实验证明，该方法是有效的。     

紫外辐照纳米尺度TiO2薄膜亲水性转变的机理

用喇曼散射光谱、扫描隧道显微学（STM）和接触角等分析方法研究了纳米尺度TiO2薄膜经365nm波长紫外辐照后的疏水－亲水转变机理。实验中观察到氧空位和OH基团的喇曼峰。用STM进行薄膜结构分析，观察到TiO2表面金红石相到锐钛矿相的结构转变。研究结果表明，纳米尺度TiO2薄膜的光致亲水性强烈地依赖于其独特的表面微结构的变化。     

Formation of substitutional metallofullerenes by laser ablation of externally doped fullerenes C60M x (M=Sm, Pt, Ni and Rh)

Photofragmentation study of metal fullerides C₆₀M _x (M = Sm, Pt, Ni and Rh) by excimer laser ablation-TOF mass spectrometry shows that metallofullerenes C_2n M and C_2n+1M formed in both the positive and negative ionic modes. The isotopic distributions of the metallofullerenes C_2n M and C_2n+1M are consistent with the calculated spectra based on the natural abundance of isotopes of C and M, confirming the formation of metallofullerenes. The metal atom is supposed to be incorporated into the network of the fullerene cage to replace one carbon atom of the cage forming substitutional metallofullerene. Odd-numbered high carbon clusters are observed in our laser ablation study of all the metal fullerides in the negative ion channel. Evolution of mass spectrum with irradiation laser shots shows that the formation of the substitutionally doped fullerenes is closely related to the production of metal carbide (MC). The structures, as well as formation mechanism, of metallofullerenes C_2n+1M and C_2n M with even and odd numbers of total atoms respectively are discussed based on the structural optimization of the odd-numbered high carbon clusters.     

Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.