聚丙烯酰胺凝胶电泳体系中蛋白质的荧光标记分析

在室温下,采用聚丙烯酰胺凝胶电泳法,分离经荧光胺或对氯荧光胺荧光标记的蛋白质样品,可在2.5小时内完成蛋白质样品的分离和其区带的鉴定。耗时是传统方法的四分之一,而且荧光图象清晰、灵敏度较高。     

Y-Ba-Cu-O系超导体的水解性研究

高转变温度T_c的Y—Ba—Cu—O系超导体是目前最引人注目的超导材料。这种超导体在液氮沸点温度以上有稳定的零电阻温度T_o。对于不同的Y、Ba和Cu组分比,只要采用相应的烧结条件,均能制备出T_c>90K的超导样品。我们在实验中发现,此种体系超导体无论组分如何,均有明显的水解特性,最终失去超导电性。水解的最终生成物由X射线衍射测定决定。另外,利用计算机对超导体完全水解的温度-时间关系进行了模拟计算。我们以组分比Y:Ba:Cu=1:2:3均匀混料,在850℃下预烧4小时(h),然后磨碎并过筛,压制成直径16mm、厚度1.5mm的圆片,在960℃下通氧烧结9小时,得到     

隔振前后振动筛对基础动负荷的理论研究

根据单自由度系统的振动理论,详细推导了振动筛对基础动负荷的计算公式;采用两个自由度系统力学模型,利用隔振理论,计算了带隔振台的振动筛对基础的动负荷,计算了采用隔振台和没有采用隔振台两种情况下对基础动负荷的比值,分析了隔振效果;为大型振动筛设计提供理论依据。     

非线性反问题求解的参数微分正则化方法

讨论了非线性反问题的求解问题,将具有大范围收敛特性的同伦方法引入到非线性反问题的求解之中,籍此克服非线性反问题常规求解过程中局部收敛的缺陷;结合吉洪诺夫正则化方法,以解决计算Frechet导数时病态的问题.在此基础上,提出了一种用于求解非线性反问题的参数微分正则化方法,给出其构造过程,并且证明了参数微分正则化方法解的存在性和收敛性.     

紫外辐照纳米尺度TiO2薄膜亲水性转变的机理

用喇曼散射光谱、扫描隧道显微学（STM）和接触角等分析方法研究了纳米尺度TiO2薄膜经365nm波长紫外辐照后的疏水－亲水转变机理。实验中观察到氧空位和OH基团的喇曼峰。用STM进行薄膜结构分析，观察到TiO2表面金红石相到锐钛矿相的结构转变。研究结果表明，纳米尺度TiO2薄膜的光致亲水性强烈地依赖于其独特的表面微结构的变化。     

Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

竞技游泳出发台出发过程中各影响因素的量化分析

运用物理学知识建立了游泳出发过程的数学模型。采用优化算法求得出发过程的最佳参数，并对出发台出发过程中的各影响因素进行了灵敏度分析，确定了各影响因素的主次关系，为制定科学的训练计划提供了依据。     

不完全投影据图像重建的最小交叉熵算法

提出图像重建的最小交叉熵算法,详细推导了算法的有关公式,并给出程序实现框图.计算机模拟结果表明:最小交叉熵算法的性能优于最大熵算法.     

电弧放电法快速合成金刚石薄膜

本文用电弧放电等离子体ＣＶＤ快速生长金刚石薄膜。用拉曼散射，Ｘ射线衍射、电子显微镜等方法进行了结构表征。文中所论述的恒定的基板温度对沉积金刚石薄膜是非常重要的。     

超滤在染料中的应用——液状活性染料研究

本文报导了用超滤法除去染料中的盐份是可行的,初步摸索了制备液状-K 型活性染料的最佳条件.