Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

“信号与系统”与“数字信号处理”的知识点整合

信号与系统和数字信号处理是通信专业的2门核心课程,它们联系非常紧密,内容上存在一定程度上的重复。对2门课程的知识体系及其间的联系进行了分析,在此基础上对2门课程的知识结构进行了重新整合。实践表明,通过知识点的整合,有效地减少了重复的教学内容,解决了教学内容多与教学课时少的矛盾,极大地提高了授课效率。     

Progress in molecular materials and devices

Initial studies on organic semiconductors began in the early 1960s in China.In the 1970s,a specialized laboratory(Lab.No.6)was established at the Institute of Chemistry,Chinese Academy of Sciences(ICCAS),which housed the first research team engaged in this field.In 1991,Organic Solids Laboratory was     

作物氮素营养状况的高光谱遥感估测技术探析

氮素是植物必需的三大营养元素之一.合理施用氮肥已成为农业生产中最为重要的问题.随着高光谱遥感技术的兴起,快速、无损伤地对作物氮素营养状况进行监测成为可能.利用高光谱遥感技术对作物氮素营养状况估测的研究方法及进展进行了综述.     

微机控制泵阀动静态特性测试及数据处理系统

本文较为详细地介绍了微机控制泵阀动静态特性测试及数据处理系统的硬件配置、软件编程和接口技术，其理论和实践具有较广泛的应用价值     

杯[4[芳烃环氧丙醚的合成

以杯「４」芳烃和环氧氯丙烷反应，合成杯「４」芳烃环氧丙醚：２５，２７－二（２‘，３’－环氧丙氧基）－２６，２８－二羟基杯「４」芳烃，并通过＾１Ｈ－ＮＭＲ，ＩＲ以及元素分析对其结构进行表征。     

IC设计中晶体管直流模型参数的提取

提出一种ＩＣ芯片设计中双极型器件直流模型参数的提取方法。采取ＧＰ模型建立双向目标函数进行全局优化提取，分１级和２级参数两层处理．１级参数系提取ＩＣ设计中各管的共同参数，２级参烽则为提取与各个管的结构和尺寸相关的特定参数，尽管提取对象只是样管，但可以由模型内在关系通过程序计算出各管的特定参数，从而保证了电路模拟所需输入参数的真实性。     

不同方法对青海粮食产需水平的预测分析

本文运用灰色预测系统，三次指数平滑法和多元回归法，对在正常年景下２０００年，２００５年和２０１０年青海粮食产需水平进行预测，并对结果作出分析讨论。     

各向异性媒质中三维涡流场的等效源法

提出计算各向异性媒质中三维涡流场的等效源法。该法是将各向异性媒质中涡流场问题转化为附加等效源的各向同性媒质中涡流场问题。还提出实现等效源法的迭代算法，该算法是用通常的求解各向同性媒质中涡流场的方法（如有限元法、边界元法、模拟源法等）迭代计算附加等效源的各向同性媒质中涡流场。文中还给出了等效源迭代算法的收敛条件。由于每次迭代都可以利用计算各向同性场的中间结果，所以迭代计算所需的机时不多。文中给出了计算实例。     

用于高效电合成过氧化氢的球形聚多巴胺改性碳毡阴极

作为最有前景的过氧化氢生产方法之一,过氧化氢的电合成越来越受到人们的重视,而适合氧气电还原合成过氧化氢的阴极材料的开发成为关键的控制因素。通过水热法制备了一种用于过氧化氢合成的球形聚多巴胺改性碳毡(ht-pDA/ACF)电极材料。在电压为2.0 V、pH为1.0的条件下,水热pDA改性碳毡阴极电解氧气还原6 h后的过氧化氢生成量达到220 mg/L,为未改性碳毡阴极的7倍。实验结果表明,pDA的引入带来了大量的缺陷位点,这些缺陷位点能够作为活性位点促进过氧化氢的合成。其次,适当聚合条件下形成的球形结构pDA有利于活性位点的暴露。该改性碳基材料制备方法成本低,为实现高效过氧化氢电合成提供材料支持。