Localization of calmodulin and calmodulin-like protein and their functions in biomineralization in P. fucata

Calmodulin (CaM) and calmodulin-like protein (CaLP) are two proteins involved in biomineralization. Their localizations in Pinctada fucata mantle epithelia were studied by Western blot (WB) analysis of the nuclear/cytosol fraction of primary cultured Pinctada fucata mantle cells and immunogold electron microscopy. The results showed a completely different distribution of these two proteins at the subcellular level. CaM was distributed throughout both the nucleus and cytoplasm of the mantle epithelium but CaLP was distributed only in the cytoplasm. The functions of these two proteins in biomineralization were investigated by shell regeneration. During this process, the expressions of CaM and CaLP were greatly enhanced in different organelles of the mantle epithelium. Overexpression of these two proteins and a mutant of calmodulin-like protein (M-CaLP) that lacks an extra C-terminal tail in MC3T3-E1 promoted the mRNA expression of osteopontin, a biomineralization marker for osteoblasts. All of the results indicated that CaM and CaLP have completely different distributions in the mantle epithelium and affect the biomineralization process at different levels. The extra C-terminal tail of CaLP is important for its functions in biomineralization in Pinctada fucata.     

模仿的定义、重要性及其分类

目的是总结模仿研究领域的主要研究成就,以为未来的学术研究建立坚实的基础。总结了模仿领域三个关键问题上的研究进展,即模仿的定义、重要性及其分类。与此同时,指出了目前关于模仿研究的三个误区。     

光合细菌在水产养殖中应用的研究进展

光合细菌是一类能进行光合作用的特殊微生物,在水产养殖中有广泛的应用.论述了光合细菌的基本特性,并重点介绍了光合细菌在净化水质、饲料添加剂和预防疾病中的相关应用.     

存活心肌的检测

心肌梗塞后是否有活性成为冠心病治疗决策的关键,而检测心肌存活性的金标准-PET又难以在临床广泛使用。国内外大量的研究表明,存活心肌的检测以多巴酚丁胺超声心动图负荷实验、多巴酚丁胺心电图负荷实验及室壁运动分析、心肌声学造影等方法的特异性、敏感性高,且操作简便、迅速、安全可靠,最值得临床推广运用。     

相变特性对MgO—PSZ热膨胀特性的影响

采用热膨胀仪研究了MgO-PSZ陶瓷材料的热膨胀特性.试样在一定条件下烧结,经550～950 ℃热处理后发生了明显的单斜相→四方相相变,此低温相变降低了MgO-PSZ的热膨胀率,其相变产生的体积收缩与受热产生的体积膨胀相互抵消,使试样表现出零膨胀特性,从而改善了材料的抗热震性能.     

钢水测氧过程锆管温度场的有限元分析

采用ANSYS软件对钢水测氧过程中测头插入钢水10 s瞬间锆管热传导过程进行模拟分析.结果表明,模拟锆管达到稳定温度的时间与实测得的温度/电动势曲线的响应时间基本相符.对不同壁厚和内径的锆管插入钢水后温度达到稳定的时间进行比较,得出厚度、内径与锆管管头、管壁内温度达到稳定时间之间的相关关系.     

热轧工艺对CSP低碳钢冷轧板成形性能的影响

通过对某钢厂CSP低碳钢冷轧基板的生产数据的统计分析,研究了化学成分、卷取温度和终轧温度对CSP低碳钢冷轧板深冲性能的影响,观察了不同终轧温度和卷取温度下冷轧基板组织的变化.结果表明,适当增加Al元素含量和减少Mn元素含量以及采用高终轧温度和低卷取温度的热轧工艺有利于成品深冲性能的提高,且冷轧基板组织检验结果证实了该热轧工艺的有效性.     

P-集合与隐形图像生成-还原

P-集合是把动态特性引入到有限普通集合中,改进普通集合得到的,P-集合(XF,XF)是一个集合对.利用P-集合生成的图像,提出P-隐形图像的概念,给出P-隐形图像的属性特征和度量特征,提出P-隐形图像生成-还原定理,并给出了P-隐形图像在图像信息系统中的应用.