鸡肝碱性磷酸酶提取及在线固定化研究

分别用有机溶剂沉淀法和盐析法从大骨鸡肝中提取出碱性磷酸酶,并考察了提取酶液的酶促反应动力学性质.在此基础上,利用顺序注射系统将提取酶在线固定于XC—72导电炭黑表面,制备了固定化碱性磷酸酶.结果表明,大骨鸡肝碱性磷酸酶催化磷酸苯二钠分解的最佳pH=10.8,最适温度为50℃,米氏常数为1.62 mmol/L.相比游离酶,所得固定化酶的比活力显著提高.     

斑马鱼SAA基因克隆及表达分析

利用巢式PCR(nest PCR)的方法克隆斑马鱼血清淀粉样蛋白A基因,并进行生物信息学分析.实时荧光定量PCR结果显示:SAA在斑马鱼胚胎发育时期的表达量呈现明显的“增长—稳定—增长”趋势.成鱼组织分布中,SAA在11个斑马鱼组织中均能检测到,在心脏和肝脏中相对高表达.     

浅谈三段式电流保护

当线路发生短路故障时,重要特征之一是线路的电流急剧增大,当电流流过某一预定值时,反应于电流升高而动作的保护装置叫过流保护。而三段式电流保护具有作为主保护和后备保护的全部功能,在实际中得到广泛应用。     

浅谈我国铁路通信信号发展趋势

随着信息时代的到来,铁路的发展也在日新月异的变化着,铁路通信信号的发展趋势正在向一体化、多信息化、功能集成化、高度自动化的方向前进。文章主要从计算机联锁设备、列车自动控制系统、调度指挥系统三个部分对信号系统进行了分析和阐述。     

祖厉河流域生态环境修复型农业经济模式探讨

通过对祖厉河流域资源配置的分析:流域内土地面积大、土层较厚、表层土壤疏松、土肥容易流失;土壤呈微碱性,土壤养分氮少、磷缺、钾适中;祖厉河流域光照充足,辐射较强,但光能利用率低,提高太阳能利用率的潜力巨大;流域内气候温凉,山、川、塬热量差异较大,川区热量富足,山、塬区热量略欠;植物生长季节短,易发生冻害;祖厉河流域降水总...     

Structure of a nanobody-stabilized active state of the β(2) adrenoceptor

G protein coupled receptors (GPCRs) exhibit a spectrum of functional behaviours in response to natural and synthetic ligands. Recent crystal structures provide insights into inactive states of several GPCRs. Efforts to obtain an agonist-bound active-state GPCR structure have proven difficult due to the inherent instability of this state in the absence of a G protein. We generated a camelid antibody fragment (nanobody) to the human β(2) adrenergic receptor (β(2)AR) that exhibits G protein-like behaviour, and obtained an agonist-bound, active-state crystal structure of the receptor-nanobody complex. Comparison with the inactive β(2)AR structure reveals subtle changes in the binding pocket; however, these small changes are associated with an 11?? outward movement of the cytoplasmic end of transmembrane segment 6, and rearrangements of transmembrane segments 5 and 7 that are remarkably similar to those observed in opsin, an active form of rhodopsin. This structure provides insights into the process of agonist binding and activation.     

Crystal structure of the β2 adrenergic receptor-Gs protein complex

G protein-coupled receptors (GPCRs) are responsible for the majority of cellular responses to hormones and neurotransmitters as well as the senses of sight, olfaction and taste. The paradigm of GPCR signalling is the activation of a heterotrimeric GTP binding protein (G protein) by an agonist-occupied receptor. The β(2) adrenergic receptor (β(2)AR) activation of Gs, the stimulatory G protein for adenylyl cyclase, has long been a model system for GPCR signalling. Here we present the crystal structure of the active state ternary complex composed of agonist-occupied monomeric β(2)AR and nucleotide-free Gs heterotrimer. The principal interactions between the β(2)AR and Gs involve the amino- and carboxy-terminal α-helices of Gs, with conformational changes propagating to the nucleotide-binding pocket. The largest conformational changes in the β(2)AR include a 14 ? outward movement at the cytoplasmic end of transmembrane segment 6 (TM6) and an α-helical extension of the cytoplasmic end of TM5. The most surprising observation is a major displacement of the α-helical domain of Gαs relative to the Ras-like GTPase domain. This crystal structure represents the first high-resolution view of transmembrane signalling by a GPCR.     

学习型组织知识创新的文化环境分析

知识创新是学习型组织的内在要求。学习型组织的知识创新是在社会系统中进行的,社会文化环境对其有重要的影响和作用。而社会环境文化的作用包括了外生变量和内生变量两种不同的方式,其中外生变量表现为适应环境的行为,内生变量表现为积极主动地创新。     

用于卫星载荷数据传输总线的终端IP核设计

根据通信卫星有效载荷数据传输总线协议及其工作原理,提出了一种总线终端IP(intellectual property)核的设计及验证方法,该IP核由总线接口模块、时钟分频模块、协议处理模块、数据采集模块等组成,其设计和研制具有自主知识产权.该IP核完成了模块仿真验证、芯片系统仿真验证和FPGA(field progra...     

基于组件式GIS的地产市场管理信息系统建设

针对地产市场状况,为了高效管理地产市场,本文在地理信息系统的基础上,利用组件式GIS二次开发技术,提出一些开发地产市场管理信息系统的设计思想,并对系统组成的模块进行了分析和设计.