杜氏藻DNA酶活性比较研究

杜氏藻(Dunaliella,常称盐藻)是一属耐高盐度的单细胞绿藻,生长迅速,不具纤维素细胞壁,是基因工程潜在的DNA(基因)受体,而其DNA酶活性高低是衡量能否作为受体细胞的重要指标,越低越好。本研究中,DNA酶指能降解DNA的多种酶的总称。 采用三聚乙醛-二苯胺法分别测定了杜氏藻属6个种的DNA酶活性。从测定结果(Tab.1)看出,杜氏藻细胞的DNA酶活性偏低,并且不同种之间存在明显差异。其中D.minuta,     

固氮酶Fe-Mo辅因子空间构型模拟——分子力学在分子模型设计中的应用

在分子模型设计中交替使用分子图形学软件和分子力学优化计算,用计算机模拟固氮酶Fe-Mo辅因子空间构型,从而为进一步的理论研究提供了一个非实验方法的有效手段。     

放矿动力学方程初探

在分析放矿过程中矿岩移动规律的基础上,提出了流动体和固结体概念。建立了矿岩块体力学模型和相应的动力学方程,应用变分原理推导出块体运动轨迹和确定流动体形状,从理论上探讨放矿动力学过程取得了新的进展。     

基于区域毁伤的基础设施网络抗毁性评估模型

基础设施网络对国家经济、人民生活和社会安全具有重要意义,为了能够准确迅速地评估基础设施网络面对物理攻击或自然灾害时的抗毁性,提出了基于区域毁伤的基础设施网络抗毁性评估模型。该模型将网络的边均分成虚拟节点,提出加权聚类算法寻找关键区域,使用归一化方法平衡网络规模和网络地理覆盖范围对评估结果的影响,算法复杂度低,运算量小,模型应用范围广,便于多个网络之间或同一网络不同规划方案的抗毁性比较。以智利电力输送网络为例,设计多组实验对评估模型进行检验,并给出可视化结果和分析。     

屈大均与佛山文化

番禺人屈大均，是明末清初的著名诗人，又是一位著作等身的学者，在岭南文化思想史上占重要的地位。他对佛山的历史、地理及人文情况十分熟悉，在他很多的诗篇和著作中均有记述和评论，为佛山人留下一份丰厚的、珍贵的文化遗产。     

Photoactivated gamma-secretase inhibitors directed to the active site covalently label presenilin 1

Cleavage of amyloid precursor protein (APP) by the beta- and gamma-secretases generates the amino and carboxy termini, respectively, of the A beta amyloidogenic peptides A beta40 and A beta42--the major constituents of the amyloid plaques in the brain parenchyma of Alzheimer's disease patients. There is evidence that the polytopic membrane-spanning proteins, presenilin 1 and 2 (PS1 and PS2), are important determinants of gamma-secretase activity: mutations in PS1 and PS2 that are associated with early-onset familial Alzheimer's disease increase the production of A beta42 (refs 4-6), the more amyloidogenic peptide; gamma-secretase activity is reduced in neuronal cultures derived from PS1-deficient mouse embryos; and directed mutagenesis of two conserved aspartates in transmembrane segments of PS1 inactivates the ability of gamma-secretase to catalyse processing of APP within its transmembrane domain. It is unknown, however, whether PS1 (which has little or no homology to any known aspartyl protease) is itself a transmembrane aspartyl protease or a gamma-secretase cofactor, or helps to colocalize gamma-secretase and APP. Here we report photoaffinity labelling of PS1 (and PS2) by potent gamma-secretase inhibitors that were designed to function as transition state analogue inhibitors directed to the active site of an aspartyl protease. This observation indicates that PS1 (and PS2) may contain the active site of gamma-secretase. Interestingly, the intact, single-chain form of wild-type PS1 is not labelled by an active-site-directed photoaffinity probe, suggesting that intact wild-type PS1 may be an aspartyl protease zymogen.     

Asseessment of the Impact of the Qinghai-Tibet Railway on the Tourist Scenery in Tibet

Theqinghai TibetPlateauisendowedwithnaturalandgeographicconditions.These conditionsshapeupmanyuniquetouristresources,suchascolorfulgeomorphology,plentifulanduniquewildanimalsandplantsand pureandsimplefolkcustomwithancientculture,etc.Butasthetrafficisnotconvenientandthe trafficcostisveryhigh,toalargeextentthislimitsthedevelomentofthelocaltourism.Thelinesuccessivelypassesthroughtheplacesof culturalinterest,likenaturallyecologicallandscapesandagriculturalareas,semi agriculturalandmemi pastoralar…     

一类多变量传感器故障容错PID控制器的设计与应用

针对一类多变量非线性系统的传感器故障,提出了一种新型的基于神经网络模型的故障容错PID控制方法.该方法应用加权移动平均残差技术对传感器故障进行检测,并利用神经网络建模技术在线重构故障传感器信号,重组PID控制器反馈回路,实现容错PID控制.通过在三水箱过程设立多种不同类型的传感器故障,进行仿真研究.仿真研究结果表明,所提出的故障检测与容错控制策略具有很强的鲁棒性和容错能力.