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71.
TANG Jianguang WANG Yongjun ZHANG Hainan WANG Chunyu HU Zhiping XUE Zhigang XIA Kun SHI Xiaoliu 《自然科学进展(英文版)》2006,16(9):930-935
Phosphorylation/dephosphorylation is probably the most common and important reversible post-translational modification of proteins. Analyzing the functional effects of phosphorylation is helpful for understanding the biological functions of proteins. Identification of the phosphorylation sites of phosphorylated protein is a prerequisite for research on phosphorylation. In this work, an effective and simple method of identification of protein phosphorylation sites has been developed. Phosphopeptides were selectively enriched with immobilized metal affinity chromatography (IMAC) and subsequently chemically modified by 4-sulfophenyl isothiocyanate, and then the chemically modified phosphopeptides were sequenced with post-source decay (PSD) matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry for detecting phosphorylation sites. The charge of derivatization by 4-sulfophenyl isothiocyanate introduces a negative sulfonic acid group at the N-terminus of a peptide, and enables the selective detection of only a single series of C-terminal y-type ions. This chemically assisted method greatly simplifies the extremely complex pattern of PSD fragment ions and makes the PSD spectra more easier to be interpreted. The phosphorylation sites of a synthesized model phosphopeptide and human c-myc protein have been successfully identified by this method. 相似文献
72.
上市公司经营者报酬与公司绩效实证研究 总被引:4,自引:1,他引:4
在介绍国外经营者报酬理论研究成果的基础上,实证分析了中国上市公司经营者报酬对公司绩效的影响,发现中国上市公司经营者持股对公司绩效没有显著性影响,而经营者年薪对公司绩效具有明显的正向作用,同时发现董事会结构及股东性质对公司经营者所采用的报酬形式具有不同的影响,最后提出了建立适合中国上市公司实际情况的经营者报酬体系的一些建议。 相似文献
73.
研究了红枣试管苗在炼苗过程中光合特性的变化规律。结果表明,在炼苗过程中,试管苗叶绿素含量增加,蒸腾强度和气孔密度降低;通过炼苗,可使试管苗的光合特性发生显著变化,增强其对环境的适应性,提高成活率。 相似文献
74.
根据水平管外蒸气轴向流动速度随轴向距离的增加而逐渐减小的流动特性 ,建立了边界层外蒸气轴向流动速度方程 ,通过对数学模型的数值求解 ,得到了无量纲液膜厚度及凝结换热系数的数值解 ,同时得出了蒸气轴向流动速度随轴向坐标 z的变化关系 .并通过实验进行了验证 ,理论计算结果与实验结果吻合得较好 .通过数据整理得到了实验关联式 . 相似文献
75.
钻柱纵向自由振动研究 总被引:1,自引:0,他引:1
提出了钻柱纵向自由振动的离散力学模型 ,根据Lagrange方程推导建立了相应的数学模型和特征值的计算式 ,并用实际算例进行了计算 .计算结果表明 ,按离散模型确定的固有振动圆频率比按连续等直杆模型确定的固有振动圆频率略小 .考虑到实际钻柱系统的非等直性 ,可以认为离散模型的精度将高于连续等直杆模型的精度 相似文献
76.
The Louzidian normal fault occurs as the eastern detachment fault of the Kalaqin metamorphic core complex. Field observations
and microstructural analyses reveal that the Louzidian-Dachengzi ductile shear zone developed in its lower-plate was genetically
related to sinistral strike-slips and extensional faulting. Two samples from this ductile shear zone yield 40Ar-39Ar plateau ages of 133 Ma (Bi) and 126 Ma (Kp), which are concordant with their isochron ages. The plateau age of 133 Ma (Bi)
records the formation age of the ductile shear zone. The inconsistent relationship between the earlier strike-slip ductile
shear zone and the later normal fault makes the Kalaqin Quasi-metamorphic core complex distinctive from Cordilleran metamorphic
core complex. These ages provide important geochronological data for putting constraints on the formation age and genesis
of such ductile shear zones. 相似文献
77.
Wu Lin Li Hong Chen Shao-feng Qin Yi-min Zong Zhi-ming Wei Xian-yong Zou Guo-lin 《武汉大学学报:自然科学英文版》2002,7(2):227-230
The reaction of acenaphthene with nitrobenzene was investigated in the presence of AlCl3. The results showed that the reaction proceeded via carboncation-electrophilic substitution reaction and free radical substitution
reaction pathway. The products of acenaphthenyl phenylamine and biacenaphthyl could be synthesized by this reaction. The influence
of the amount of AlCl3 and the temperature on the components of products were also studied in this reaction.
Foundation item: Supported by the National Nature Science Foundation of China (No. 29676045)
Biography: Wu Lin (1965-), male, Postdictor, research direction: PAHs catalytic conversion and its biologic toxicity. 相似文献
78.
Two ductile shear zones trending EW and NNE respectively not only controlled the tectonic framework of the northern North
China, but also constrained the geodynamic background for gold mineralization in this region. Field observations and microstructural
analyses reveal that the EW trending ductile shear zones are mainly contributed to dextral compressional deformation resulting
from top-to-the-southeast oblique thrust shearing, whereas the NNE trending ones are genetically related to sinistral strike-slip
and extensional faulting. One sample from the former yielded an 40Ar–39Ar plateau age of (219± 4) Ma (Bi) and two samples from the latter gave 40Ar–39Ar plateau ages of (116± 2) Ma (Bi) and (127±3) Ma (Bi). These ages provide constraints on the top-to-the-southeast oblique
thrusting event occurring in Late Triassic and the sinistral extensional and strike-slip faulting event which occurred in
Early Cretaceous. 相似文献
79.
Two ductile shear zones trending EW and NNE respectively not only controlled the tectonic framework of the northern North China, but also constrained the geodynamic background for gold mineralization in this region. Field observations and microstructural analyses reveal that the EW trending ductile shear zones are mainly contributed to dextral compressional deformation resulting from top-to- the-southeast oblique thrust shearing, whereas the NNE trending ones are genetically related to sinistral strike-slip and extensional faulting. One sample from the former yielded an 40Ar-39Ar plateau age of (219±4) Ma (Bi) and two samples from the latter gave 40Ar-39Ar plateau ages of (116±2) Ma (Bi) and (127±3) Ma (Bi). These ages provide constraints on the top-to-the-southeast oblique thrusting event occurring in Late Triassic and the sinistral extensional and strike-slip faulting event which occurred in Early Cretaceous. 相似文献
80.
重庆市兽类资源及其区系分析 总被引:3,自引:0,他引:3
重庆市目前已知兽类117种,隶属于9目、31科、79属,其中,东洋界种88种,古北界种19种,季风型及广布种10种,地理分布上处于盆中平原丘陵省的物种仅54种,盆地东北缘大巴山省有85种,盆地东南缘山地省有79种,同时还探讨了重庆市兽类的生物多样性和资源类型。 相似文献