Interactions between the nuclear matrix proteins and the 5′-flankingcis-acting elements of the human ε-globin gene

An erythroid-specific nuclear matrix protein (termed ε-NMP_k) in K562 cells, which can specifically bind to the positive stage-specific regulatory element (ε-PRE II, - 446 - 419 bp) upstream of the human ε-globin gene, has been identified by using gel mobility shift assay. Meanwhile, Southwestern blotting assay showed that the nuclear matrix protein ε-NMP_k in K562, cells may be composed of two polypeptides (∼ 40 ku). In addition, it is observed in the gel mobility shift assay that the nuclear matrix proteins from K562, HEL and Raji cells can bind to the silencer DNA (- 392 - 177 bp) in the 5′-flanking sequence of human ε-globin gene respectively. However, the shift band K detected in K562 cells is different from shift band H/R in HEL and Raji cells, suggesting that a common nuclear matrix protein may exist in HEL and Raji cells. Results show that the nuclear matrix protein may play an important role in the regulation of the human ε-globin gene expression.     

下腹部预扩张皮瓣治疗手部烫、烧伤后疤痕挛缩畸形12例临床分析

目的:探讨下腹部预扩张皮瓣治疗手部烫烧伤后疤痕挛缩畸形的临床效果。方法:2007年10月至2011年6月应用下腹部预扩张皮瓣治疗手部烫烧伤后疤痕挛缩畸形12例。结果:功能矫正满意,疤痕切除完整,随访半年无疤痕增生及挛缩出现。结论:下腹部预扩张皮瓣治疗手部烫、烧伤后疤痕挛缩畸形是一种远期效果肯定的手术方法。     

浅谈透皮贴剂的发展

铬物质是潜在的降糖药物,文章通过荧光光谱技术研究了磺基水杨酸铬配合物与牛血清白蛋白的作用,揭示了在药物代谢过程中,血清白蛋白起到很重要的作用.     

耦合模块度优化与谱聚类的供水管网分区算法

为降低供水管网漏损,实现供水管网快速准确分区,提出一种耦合模块度优化与谱聚类的供水管网分区算法。该算法采用快速模块度优化算法对供水管网进行社区划分,以每个社区为节点、社区间连接关系为边,结合各社区内的水力特征和空间区位特征计算差异性作为边权重,构建对偶图。利用谱聚类算法完成供水管网分区。结果表明,该算法求解的管网分区结果相比快速模块度优化和谱聚类算法,将空间上更邻近的管段划分在同一分区,不会产生狭长型无效分区,且在模块度及边界管道数量上表现较为均衡,管网分区方案不仅模块度高,而且边界管道数量少。     

舰船空气尾流场特性及其控制技术研究综述

舰船空气尾流场是影响舰载机安全起降的重要因素之一;其相关研究在舰载机作战、舰船操纵、海上航空气象保障等领域具有重要意义。总结了无耦合条件下、舰船/舰载机耦合条件下的舰船空气尾流场特性相关研究成果,分析了当前舰船空气尾流场的控制技术研究现状;并提出基于射流技术的新型舰船空气尾流场控制技术;可为舰船空气尾流场特性和控制技术研究提供有益的参考。     

A novel ubiquitin ligase is deficient in Fanconi anemia

Fanconi anemia is a recessively inherited disease characterized by congenital defects, bone marrow failure and cancer susceptibility. Cells from individuals with Fanconi anemia are highly sensitive to DNA-crosslinking drugs, such as mitomycin C (MMC). Fanconi anemia proteins function in a DNA damage response pathway involving breast cancer susceptibility gene products, BRCA1 and BRCA2 (refs. 1,2). A key step in this pathway is monoubiquitination of FANCD2, resulting in the redistribution of FANCD2 to nuclear foci containing BRCA1 (ref. 3). The underlying mechanism is unclear because the five Fanconi anemia proteins known to be required for this ubiquitination have no recognizable ubiquitin ligase motifs. Here we report a new component of a Fanconi anemia protein complex, called PHF9, which possesses E3 ubiquitin ligase activity in vitro and is essential for FANCD2 monoubiquitination in vivo. Because PHF9 is defective in a cell line derived from an individual with Fanconi anemia, we conclude that PHF9 (also called FANCL) represents a novel Fanconi anemia complementation group (FA-L). Our data suggest that PHF9 has a crucial role in the Fanconi anemia pathway as the likely catalytic subunit required for monoubiquitination of FANCD2.     

碱式反应氢化物发生—ICP光谱法测定矿物中锗的含量

含ＮａＢＨ４的碱性锗试液和酸反应（碱式反应），也能产生ＧｅＨ４，产率和传统的含锗酸性试液与ＮａＢＨ４反应（酸式反应）相同。碱式反应的突出优点是能消除ⅤⅢ和ＩＢ族元素对锗氢化的严重化学干扰。用碱式反应氢化物发生－ＩＣＰ光谱法测定精铜和锌渣中含锗量，结果较好。本方法的回收率为９７．２％，检出限为２．５ｎｇ／ｍｌ。     

Development of a nurse robot serving in infectious disease isolation wards

A prototype of nurse robot system serving in infectious disease wards is developed by analyzing the systems requirement. Firstly, the type synthesis and dimension synthesis for optimizing the workspace are presented. Secondly, the tele-control system based on velocity control mode is introduced, and tele-control program is written. Finally, the imitation of position workspace and experiment of transforming objects from buffer area to isolation area are carried out.     

过氧化氢酶在DNA修饰的热解石墨电极上的直接电子转移

通过将单、双螺旋的小牛胸腺DNA膜修饰在热解石墨电极上,研究了过氧化氢酶的直接伏安和电催化性质.结果表明过氧化氢酶(Cat)-DNA膜上显示出一对很好的几乎可逆的循环伏安峰,在50 mmol·L,pH为7.0磷酸缓冲溶液中,扫描速度为0.1 V·s-1情况下,单、双螺旋DNA膜上,其式电位分别为-0.223 V和-0.209V,其还原和氧化电子传递速率常数值分别为ks,red=21.0 s-1;ks,ox=33.0 s-1和ks,Red=13.5 s-1;ks,ox=13.3 s-1.在pH为3.81～7.72的范围内,Fe(Ⅲ)/Fe(Ⅱ)电对的还原电位随pH呈线性变化,表明一电子的转移伴有一质子的耦合.另外从紫外可见吸收光谱可见,在中性pH条件下,过氧化氢酶在DNA膜中保持了原有的结构不变.此外,我们还研究得到在DNA膜中过氧化氢酶保持了对过氧化氢的催化活性,并提出了可能的催化机制.     

柔性基础上主动隔振执行器安装方式的动力学研究

研究了积极隔振和消极隔振情况下，柔性基础上主动隔振执行器在并联结构安装方式和基础消振结构安装方式下的动力学特性，分析比较了不同安装方式的主动控制力与被动隔振系统及柔性基础之间的参数关系，为主动隔振器安装方式的选取提供了科学依据。