Synthesis of Thermoresponsive Poly（ diethyleneglycol methacrylate-co-6-Ovinyladipoyl-D-glucopyranose） Glycopolymer via RAFT Polymerization

The monomer 6-O-vinyladipoyl-D-glucopyranose（ VAG）was synthesized by lipase catalyzed trans-esterification of divinyladipate with D-glucopyranose. A novel double hydrophilic glycopolymer poly（ diethyleneglycol methacrylate-co-6-Ovinyladipoyl-D-glucopyranose）（ P（ DEGMA-co-VAG）） with narrow polydispersity（ PDI） and thermosensitivity was prepared by reversible addition-fragmentation chain transfer（ RAFT）polymerization. P（ DEGMA-co-VAG） was characterized by1 H NMR,FTIR and gel permeation chromatography（ GPC）. The characterization of UV-visible spectroscopy showed that the micelles from glycopolymer P（ DEGMA-co-VAG） were thermo-responsive and the low critical solution temperature（ LCST） could be controlled by the molar ratio of monomers. When the molar ratio of DEGMA and VAG was 2∶ 1,the LCST of P（ DEGMA-co-VAG） was36 ℃ in aqueous solution,which could form nano micelles in the human body environment. It was found that P（ DEGMA-co-VAG）was non-toxic at 0. 1-1 mg / m L concentrations when incubated with pig iliac endothelial cells（ PIECs） for 24 h. Thus,the synthesized glycopolymers has great potential as drug delivery carriers.     

试论民办高校大学生参与科研研究——无线mesh团队学生参与项目纪实

民办高技是我国高等教育的后起之秀,这就决定了它要在当前高校扩招、生源质量和数量面临急剧下降的严峻形势下求得生存与发展.就必须要以市场为导向,以培养应用型人才为己任,以全面提高教学质量为突破口.通过特色专业建设规划和课程改革,以应用能力为主线构建学生的知识、能力、素质结构与培养方案,加强实践性教学环节,注重学生项目科研等实践性课程中实际应用能力和创新能力的培养.     

Development and application research of light weight heat treated B-grade bulletproof steel

The light weight heat treated B-grade bulletproof steel was developed through composition design and optimization based on multiplex alloying, multiplex micro-alloying design ideas and complex phase structure strengthening theory. The puzzle how to avoid the quenching deformation problem of super high strength thin sheet was solved through heat treatment in a die with a set of cooling system. Such B-grade bulletproof steel plate has fine tempered lath martensite structure. The shooting and certification test results showed that the shoot resistance of B- grade bulletproof steel plate can meet the protection demand of Protection specification for cash carrying vehicles （GA 164--2005）. In comparison with B-grade bulletproof steel plate made by one of the companies in Sweden, the weight of the developed B-grade bulletproof steel plate can be decreased by 8 % under the same shoot resistance condition. It will be meaningful for cash truck and anti-hijacking vehicle to realize light weight, energy conservation and emission reduction.     

车轮宽度对轿车风阻的影响

针对某三厢轿车,采用计算流体动力学(CFD)数值计算方法,研究车轮宽度对整车气动性能的影响.通过综合分析不同宽度孤立车轮周围的流场结构变化及具有不同宽度车轮的整车周围流场的结构特性,得到结论:车轮宽度每减小5%,单车轮模型气动阻力约减小9.2%,整车模型气动阻力约减小2%.这是因为减小车轮宽度可以减小车轮两侧的气流分离,缩小尾部涡流区域,降低车轮及汽车尾部湍流强度,从而有助于降低车轮及整车气动阻力.     

旋转车轮对整车气动性能的影响评价

为研究整车轮边流场结构特征,以不同尾部造型形式的简单车体和复杂车体为研究对象,分别对静止和旋转车轮工况进行了数值研究.计算采用定常雷诺时均纳维斯托克斯方程.针对数值计算结果,通过对静止及旋转车轮周围流场的流动情况、表面压力系数、气动阻力系数和升力系数等数据的详细分析,得到了车轮旋转会对轮边流场和整车流场产生极大的影响,整车气动阻力和气动升力下降,气动性能得到改善.     

涡流发生器对3/4汽车模型风洞流场品质的影响

采用数值计算的方法对3/4开口回流式汽车风洞喷口处的涡流发生器进行了研究.对比研究了三种不同片数的涡流发生器对流场品质的影响.首先采用定常雷诺时均纳维斯托克斯方程求解了流场的定常特性,接着采用大涡模拟(LES)方法对流场的非定常特性进行了研究.并就两种计算方法给予了相应的试验验证.对比计算结果得到涡流发生器可以在一定程度上提高流场均与性,尤其是三片式涡流发生器的工况能较好地拓宽风洞测试区的高流速区域的范围,降低测试区的静压梯度,减小试验段内的湍流度和剪切层内的湍流度,延长测试段内低湍流区的长度,分散流场内的脉动能量,降低低频颤振敏感频率区的能量聚集.     

鄱阳湖滨湖区污染控制区划与防治对策研究

实行分区污染控制与管理是开展鄱阳湖滨湖区污染综合防治的有效措施。基于鄱阳湖滨湖区主要污染物来源分析和污染控制区划原则,对鄱阳湖滨湖区的污染控制区划进行了探讨,在各类型污染源分区叠加基础上,确定滨湖区污染控制区划,并根据各分区污染负荷特征提出了相应的分区控制措施。结果表明,鄱阳湖滨湖区主要污染物氮、磷、CODCr和氨氮主要来源于畜禽养殖和城镇生活污染。依据各类型污染源影响,可将鄱阳湖滨湖区划分为工业与生活污染控制区、农业养殖污染控制区和农业种植面源综合整治区。并将各区进一步细分为重点控制区和一般控制区。针对各区污染特征提出了相应的污染控制对策,以期为鄱阳湖滨湖区污染防治工作提供帮助。     

Observation of directional change of core field inside flux ropes within one reconnection diffusion region in the Earth’s magnetotail

Two consecutive magnetic flux ropes, separated by less than 30 s （△t 〈 30 s）, are observed within one magnetic reconnection diffusion region without strong guide field in the Earth＇s magnetotail by Cluster multispacecraft. The flux ropes are characterized by bipolar signatures of the south-north magnetic field component Bz accompanied with strong core magnetic field By, intense current J and density depletions inside of them. In spite of the small but non-trivial global scale negative guide field （-By）, there exists a directional change of the core fields of two flux ropes, i.e., -By for the first one, and ＋By for the second one. The directions of the core fields are the same as the ambient cross-tail magnetic field component （By） just outside of flux ropes. Therefore, we suggest that the core field of flux ropes is formed by compression of the local preexisting By and that the directional change of core field is due to the change of local preexisting By. Such a change in ambient By might be caused by some microscale physics.     

小麦GLB1蛋白单克隆抗体的制备及鉴定

以小麦主要过敏原Glb-1蛋白为免疫原免疫BALB/c小鼠,取免疫小鼠脾细胞与小鼠骨髓瘤NS-1细胞融合。采用细胞融合和有限稀释法相结合的方法快速筛选获得稳定分泌的特异性杂交瘤细胞株,用杂交瘤细胞株诱生小鼠腹水并用蛋白A亲和层析法纯化抗体后检测。采用间接ELISA法鉴定该单克隆抗体的IgG亚型;通过间接ELISA鉴定该单克隆抗体的特性和交叉性。利用双单抗夹心ELISA法检测单抗的抗原表位特异性。结果表明:共获得4株可稳定分泌小鼠抗小麦主要过敏原Glb-1蛋白的单克隆抗体,分别命名为1C4、4H5、1A9、4F5,经检测其Ig亚型均为IgG1,且4株单抗效价均在10-9以上。ELISA结果分析表明该4株单抗均能特异性识别小麦主要过敏原Glb-1蛋白且和其他常见食物无交叉反应性。将4株单抗两两配对进行ELISA实验,结果发现1C4与4H5可能有不同的抗原表位,以此建立的双抗夹心ELISA系统可以检测小麦Glb1-G3蛋白。实验成功制备了鼠抗小麦主要过敏原Glb-1蛋白抗原的单克隆抗体,并且建立了双单抗夹心ELISA检测系统,为小麦主要过敏原蛋白的检测奠定了基础。     

In fl uence of HEPES buffer on the local pH and formation of surface layer during in vitro degradation tests of magnesium in DMEM

The human body is a buffered environment where p H is effectively maintained. HEPES is a biological buffer often used to mimic the buffering activity of the body in in vitro studies on the degradation behavior of magnesium. However, the influence of HEPES on the degradation behavior of magnesium in the DMEM pseudo-physiological solution has not yet been determined. The research aimed at elucidating the degradation mechanisms of magnesium in DMEM with and without HEPES. The morphologies and compositions of surface layers formed during in vitro degradation tests for 15–3600 s were characterized. The effect of HEPES on the electrochemical behavior and corrosion tendency was determined by performing electrochemical tests. HEPES indeed retained the local p H, leading to intense intergranular/interparticle corrosion of magnesium made from powder and an increased degradation rate. This was attributed to an interconnected network of cracks formed at the original powder particle boundaries and grain boundaries in the surface layer, which provided pathways for the corrosive medium to interact continuously with the internal surfaces and promoted further dissolution. Surface analysis revealed significantly reduced amounts of precipitated calcium phosphates due to the buffering activity of HEPES so that magnesium became less well protected in the buffered environment.