RNAi-mediated knockingdown of rlpk2 gene retarded soybean leaf senescence

Leaf senescence that occurs in the last stage of leaf development is a genetically programmed process. It is very significant to elucidate the molecular mechanisms that control the initiation and progression of leaf senescence and the way the senescence signal is transduced. In a previous study on artificially induced soybean leaf senescence, we cloned a novel gene designated rlpk2 (Genbank Accession No. AY687391) that encodes a leucine-rich repeat (LRR) receptor like protein kinase. The expression level of rlpk2 gene was shown to be strongly up-regulated during both the natural leaf senescence process in this report and the artificially induced primary-leaf-senescence process in our previous work. The RNA interference (RNAi)-mediated knocking-down of rlpk2 dramatically retarded both the natural and nutrient deficiency-induced leaf senescence in transgenic soybean. The transgenic leaves showed more cell-aggregated surface structure and higher content of chlorophyll.     

英语阅读能力的培养

学习英语的目的就是要掌握用英语进行听、说、读、写等交际活动的能力，即“四会”，而“读”是其他“三会”的基础。从阅读方法、阅读习惯和阅读技巧3个方面阐述了如何培养和提高英语阅读能力.     

多层次的FCE在高校教师职称评定中的应用

职称评定工作是高等学校的一项重要工作,它受到了教师的普遍关注,评定的合理与否,将直接影响到高校师资队伍的建设和发展.现针对高校教师职称评定问题,将层次分析法应用于模糊综合评价中,建立了多层次的模糊综合评价模型,给出了高校教师职称评定的量化方法,使职称评定工作尽可能地减少主观因素的影响.     

L光滑拓扑空间中弱映射的强形式

在L光滑拓扑空间中给出了L光滑强不定映射、强不定开映射、强不定闭映射及L光滑强半连续映射、强半开闭映射，并讨论了它们的等价刻画和相互关系。     

基于J2EE的藏汉英电子词典的研究

文章就汉藏英网络词典的研发、J2EE核心技术及数据库的设计,通过比较各种索引结构及查找算法,提出本系统的索引结构及算法,同时介绍了系统的主要功能.     

Functional characterization of cadmium-responsive garlic gene AsMT2b： A new member of metallothionein family

METALLOTHIONEINS(MT)ARE LOW-MOLECULAR-WEIGHT AND CYSTEINE-RICH PROTEINS.THE FIRST MT PROTEIN WAS ISOLATED FROM EQUINE RENAL CORTEX IN1957[1].GENES EN-CODING MTS OCCUR IN DIVERSE ORGANISMS SUCH AS ANIMALS,HIGHER PLANTS,EUKARYOTIC MICROORGANISMS AND IN SOME…     

Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys

According to the UNAIDS/WHO report (http:// www.unaids.org/Epi2005/doc/report.html), there are about 40.3 million HIV/AIDS survivors, and the new HIV infection number amounts to about 4.9 million, while the death number is some 3.1 million in the world in…     

Criterions and Measures of Route Selection of Shallowly Embedded Long.Distance Oil and Gas Pipeline in Mountain Areas

According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows： the flat landform is the first choice in route alignment. The foot of mountain is the first choice when the route passes by the valley. The route should pass by but the shady and deposited slope and not in sunny and erosive slope as possible as it can. The pipeline should be vertical to contour climbing and descending the mountain except steep slope. Tunnel can be used in crossing foothill. Perpendicularly traversing the river is better than beveling; the worst choice is to put the pipeline along the river. Bypass is the best choice in karsts area. The order of route selection should be pre-choosing, investigation, optimization and adjustment.     

蓝色圆珠笔油墨的HPLC鉴别

本文采用高效液相色谱法对国内外生产的各种蓝色圆珠笔油墨的种类进行了鉴别。采用ZORBAXBonus-RP柱,流动相为乙腈-水,检测波长为580nm,柱温25℃,本法简便,准确,灵敏度高,重复性好,可用于蓝色圆珠笔油墨种类的鉴定。