hIL-2／IFN-1嵌合基因原核表达质粒的构建与表达

为构建hIL 2 /IFN γ 嵌合基因原核表达质粒 ,筛选其高效表达工程菌 ,设计特异性引物 ,PCR扩增目的基因并克隆至pPROEXHTb质粒NcoⅠ /HindⅢ位点 ,经双酶切及序列测定筛选阳性克隆 ,IPTG诱导工程菌后以SDS PAGE电泳鉴定目的蛋白的表达情况 ,并测定其抗原性和生物学活性 ,结果成功构建了工程菌Top10F’[hIL 2 /IFN γ],经优化表达条件后 ,目的蛋白表达量约占菌体总蛋白的 30 % ,分子质量约为 34ku .ELISA和Western blot实验结果表明 ,目的蛋白能特异性结合抗IFN γ 抗体 ,呈阳性反应 ,生物学活性测定显示其IL 2和IFN γ 比活性分别为 1 7× 10 7U/mg与 3 2× 10 6 U/mg .     

动名兼类和动词名用的区别

本文力求说明汉语动名兼类词和动词名用的区别，并根据双音节复合动词与数量词组(几)“个”的组合能力来划分两者的界限。     

Velocity Distribution of Slurry in Horizontal Pipe When Solid Particles Sliding

Based on the laws of momentum conservation and impulse in accelerating process, the distribution on speed of ununiform slurry flow in a horizontal pipe was studied. According to the momentum change of solid particles and conveying liquid of slurry flow during accelerating, and some effect factors, the relationship between the speed of solid particles and the speed of conveying liquid was obtained.When dealing with the friction between sliding solid particles and pipe, it is pivotal to reasonably distribute component of friction to each solid particle. The friction coefficient between solid particles was obtained by forces analysis and theoretic calculation, and can be used to calculate the friction force on every solid particle. The effect of friction on speed of ever), solid particle was investigated through the impulse law. The result is more accurate than that by using uniform friction on solid particles. It is completely new method to use above theory to get solid particles speed distribution, conveying liquid speed distribution and slurry speed distribution.     

Efficient Feature Extraction Using Apache Spark for Network Behavior Anomaly Detection

Extracting and analyzing network traffic feature is fundamental in the design and implementation of network behavior anomaly detection methods. The traditional network traffic feature method focuses on the statistical features of traffic volume. However, this approach is not sufficient to reflect the communication pattern features. A different approach is required to detect anomalous behaviors that do not exhibit traffic volume changes,such as low-intensity anomalous behaviors caused by Denial of Service/Distributed Denial of Service(Do S/DDo S)attacks, Internet worms and scanning, and Bot Nets. We propose an efficient traffic feature extraction architecture based on our proposed approach, which combines the benefit of traffic volume features and network communication pattern features. This method can detect low-intensity anomalous network behaviors and conventional traffic volume anomalies. We implemented our approach on Spark Streaming and validated our feature set using labelled real-world dataset collected from the Sichuan University campus network. Our results demonstrate that the traffic feature extraction approach is efficient in detecting both traffic variations and communication structure changes.Based on our evaluation of the MIT-DRAPA dataset, the same detection approach utilizes traffic volume features with detection precision of 82.3% and communication pattern features with detection precision of 89.9%. Our proposed feature set improves precision by 94%.     

基于AHP的产城融合评价研究

通过阐述我国产城融合发展中存在的问题,筛选出影响产城融合的指标因素,构建了包括经济发展、城市化水平和配套设施3个指标在内的评价体系.利用AHP方法进行定性和定量分析,确定了指标的权重,同时建立了产城融合评价模型,可以明确城市发展阶段,为促进城市产城融合建设提供了一些思路.     

Backstepping approach to the adaptive regulator design for a one-dimensional wave equation with general input harmonic disturbance

This paper considers the parameter estimation and stabilization of an unstable one-dimensional wave equation with matched general harmonic disturbance at the controlled end. The backstepping method for infinite-dimensional system is adopted in the design of the adaptive regulator. It is shown that the resulting closed-loop system is asymptotically stable. Meanwhile, the estimated parameter is shown to be convergent to the unknown parameter as time goes to infinity.     

Glutenin macropolymer particles size distribution of six wheat varieties in eastern China

Size distribution of glutenin macropolymer(GMP) particles significantly affects the quality of final products in wheat. Six wheat varieties in eastern China were used for investigating the GMP content, particle size distribution and their relation. The results showed that the particle diameter of GMP changed from 0.38 μm to 269 μm. A typical bimodal curve of volume distribution of particles with peak values ranged from 4.44 μm to 5.36 μm and from 45.76 μm to 116.30 μm, respectively. Number distribution of particles showed the typical population with peak values in the range of 0.57-1.00 μm. Proportions of granules 12 μm, 12-80 μm and 80 μm were in the range of 17.1%-47.8%, 32.1%-50.3% and 10.2%-38.1% of total volume, respectively. The content of GMP was negatively correlated with the volume of GMP particles 12 μm, but positively correlated with the volume of particles 80 μm. The results suggested that the greater percentage of larger GMP particles is, the more GMP content there is in wheat grain.     

GPSNISNAV integrated relative navigation and attitude determination system for ultra-close spacecraft formation flying

For the improvement of accuracy and better fault-tolerant performance, a global position system (GPS)/vision navigation (VISNAV) integrated relative navigation and attitude determination approach is presented for ultra-close spacecraft formation flying. Onboard GPS and VISNAV system are adopted and a federal Kalman filter architecture is used for the total navigation system design. Simulation results indicate that the integrated system can provide a total improvement of relative navigation and attitude estimation performance in accuracy and fault-tolerance.     

腹腔镜胃癌根治术与传统胃癌根治术的临床效果比较

目的:比较腹腔镜胃癌根治术与传统胃癌根治术的临床效果。方法:回顾分析2010年10月至2013年10月大理大学附属医院行腹腔镜胃癌根治术及胃癌根治术共103例胃癌患者的临床资料,分析比较两组患者的临床效果。结果:应用腹腔镜组平均出血量为(153.05±11.71)m L,明显少于开腹手术的(278.45±12.65)m L(P<0.05)。腹腔镜组的平均术后恢复肛门排气时间(3.10±0.62)d,低于开腹手术组的(5.58±1.12)d(P<0.05)。腹腔镜组平均术后住院时间为(14.25±2.78)d,明显低于开腹手术的(22.07±3.59)d(P<0.05)。生存率:行腹腔镜手术者1年总生存率94.1%,开腹手术总生存率92.1%(P>0.05)。结论:腹腔镜胃癌根治术具有术中出血少、术后住院天数少、并发症少等优点,治疗效果与开腹手术相当。     

利用生物信息学数据及工具合成表达链霉亲和素基因

目的:人工合成表达链霉亲和素基因,制备链霉亲和素磁珠复合物用于核酸分离.方法:利用NCBI、BCM等提供的生物信息及软件工具,将链霉亲和素蛋白的基因进行密码子优化,并分割成互为重叠的小片段寡聚核苷酸链,采用化学和酶促合成相结合的方法合成编码链霉亲和素蛋白基因.将纯化的链霉亲和素通过偶联剂与带羧基的磁粒共价结合,用于快速...