一个特殊的顺式发夹核酶对感染烟草原生质体的影响

研究一个烟草花叶病毒(TMV)cDNA3′末端被特异切割的发夹核酶对感染原生质体的作用。选用一个外源基因绿色水母荧光蛋白(GFPuv)作为报道基因，取代了大部分的CP编码区域，以研究在烟草原生质体中发夹核酶对TMV载体的复制，通过GFP的表达来确定表达量。顺式发夹核酶对TMV载体感染效率的影响通过体外转录来观察。TMV GFPRIB体外转录感染原生质体的效率比与其不含该核酶的对应物的感染效率高3～5倍。Northern杂交结果表明，包括核酶在内的3’末端非病毒序列在体外转录反应开始后就被顺式发夹核酶切除了。当直接用载体DNA pSTMVGFPRIB感染原生质体时，感染原生质体的数量比与之相对应的pSTMVGFP NOS感染原生质体数多50％～80％。     

聚能射流水中侵爆盖板B炸药仿真研究

为获得聚能射流对水中盖板B炸药侵爆作用能力,开展水层厚度影响规律研究.基于准定常侵彻理论得到了紫铜聚能射流侵彻水介质时射流速度计算式;建立侵爆仿真模型,研究了聚能射流水中成型与运动、侵彻盖板及起爆装药的变化特性,计算给出了射流侵彻盖板前后的速度和直径的变化,结果表明,射流侵爆水中盖板B炸药的临界水层厚度为261 mm,并依据Held判据得出B炸药的起爆阈值为18.598 mm³·μs^-2.研究结果对聚能射流水下毁伤技术的应用提供技术参考.     

基于USB接口的涉密数据传输可信监控系统研究

针对传统基于USB接口的监控系统存在的诸如缺乏介质身份认证手段,难以有效地对各等级进行隔离,可信度低等问题,一种基于可信机制的数据传输监控系统被提出以解决上述问题.系统应用动态口令与指纹识别相结合的双向身份认证方案及文件过滤驱动技术,以“可信构件”为根构建系统内部的可信链模型,通过驱动层与应用层相结合的多级安全策略加强...     

分层饱和土中横向受荷桩动力响应分析

考虑饱和土的成层性和孔隙流体与土骨架变形的相互作用,用积分变换方法构建盘面和柱面荷载作用下土体与结构动力相互作用影响函数,用边界元方法建立相应的考虑饱和土成层性的地基与桩基础动力相互作用分析方法.以层状饱和土中横向受荷桩动力阻抗分析表明分析方法的可行性.算例分析孔隙流体对分层饱和土中横向受荷桩动力响应影响.     

黄粉虫的营养保健作用

为了解黄粉虫油脂和去油虫渣的营养保健作用,将黄粉虫油脂和去油虫渣掺入饲料,饲喂初成年的ICR小鼠,测定了其对小鼠的学习记忆能力和抗疲劳能力的影响;同时研究了黄粉虫全虫干粉对皮下注射了D-半乳糖致衰老模型小鼠的影响。结果表明:分离后的黄粉虫油脂和油渣均能提高小鼠的抗疲劳能力;但仅黄粉虫油渣可促进其学习能力。饲料中掺入黄粉虫幼虫干粉,使其在饲料中的质量分数为0.05和0.10,可抑制D-半乳糖致衰老小鼠体重的降低,显著提高其脾脏和胸腺的脏器重量,增强免疫力;但对血清抗氧化能力无明显的改善作用。     

Stepwise replication identifies a low-producing lymphotoxin-alpha allele as a major risk factor for early-onset leprosy

Host genetics has an important role in leprosy, and variants in the shared promoter region of PARK2 and PACRG were the first major susceptibility factors identified by positional cloning. Here we report the linkage disequilibrium mapping of the second linkage peak of our previous genome-wide scan, located close to the HLA complex. In both a Vietnamese familial sample and an Indian case-control sample, the low-producing lymphotoxin-alpha (LTA)+80 A allele was significantly associated with an increase in leprosy risk (P = 0.007 and P = 0.01, respectively). Analysis of an additional case-control sample from Brazil and an additional familial sample from Vietnam showed that the LTA+80 effect was much stronger in young individuals. In the combined sample of 298 Vietnamese familial trios, the odds ratio of leprosy for LTA+80 AA/AC versus CC subjects was 2.11 (P = 0.000024), which increased to 5.63 (P = 0.0000004) in the subsample of 121 trios of affected individuals diagnosed before 16 years of age. In addition to identifying LTA as a major gene associated with early-onset leprosy, our study highlights the critical role of case- and population-specific factors in the dissection of susceptibility variants in complex diseases.     

煤矿地下多层火区探测技术研究与应用

采用地面氡气测量法、瞬变电磁法和激发极化法相结合手段,经过野外数据采集、室内资料处理、理论研究和综合分析,查明了地下三层煤层自燃火区的位置与范围,取得了很好的实际应用效果.     

Switch from protective to adverse inflammation during influenza: viral determinants and hemostasis are caught as culprits

Influenza viruses cause acute respiratory infections, which are highly contagious and occur as seasonal epidemic and sporadic pandemic outbreaks. Innate immune response is activated shortly after infection with influenza A viruses (IAV), affording effective protection of the host. However, this response should be tightly regulated, as insufficient inflammation may result in virus escape from immunosurveillance. In contrast, excessive inflammation may result in bystander lung tissue damage, loss of respiratory capacity, and deterioration of the clinical outcome of IAV infections. In this review, we give a comprehensive overview of the innate immune response to IAV infection and summarize the most important findings on how the host can inappropriately respond to influenza.     

基于组件的分布式虚拟仿真平台的研究与实现

分布式环境下虚拟仿真技术是在虚拟的逼真环境下,对产品设计及其周边环境进行协同仿真验证的有效手段.本文首先提出了分布式虚拟仿真系统设计的一些概念和原理,接着提出了基于组件对象模型的虚拟仿真系统建模与仿真方法,并重点阐述了支持分布式虚拟仿真的集成框架及其关键实现技术,该平台在一具体的仿真实验系统—飞机模拟仿真飞行系统的的设计中得到具体应用。     

国际科技合作融入全球创新网络研究评述与展望

对近些年国际科技合作融入全球创新网络研究文献进行综述,依次从国际科技合作融入全球创新网络的内涵价值、影响因素、基本途径及我国的政策选择等层面逐步展开评述,试图为学术研究者和政策制定者对该领域的研究脉络、研究内容、主要观点和前景展望提供一个比较清晰的认识,最后提出进一步探究国际科技合作融入全球创新网络的内在机理,发掘经济...