Effect of monoclonal antibody to bull sperm sp18 on murine fertilization and embryos development

Western blot analysis revealed that one IgG1 monoclonal antibody (mAb) to sp18 family membrane proteins (Mr. 14, 16 and 18 ku) of bovine sperm reacted faintly with protein bands of 14, 18, 22, 30 and 60 ku (reducing) in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence (IIP) showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization (IVF) and embryo development trails, a total of 426 oocytes from C57BL/6 and F1 hybrid strain (CD1 × C57BL/6 cross) of 12 female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15-20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1 %, which was not significantly (P > 0.05) different from the nonspecific mouse IgG (79.2%) and non-IgG (80.3 %) control groups. Fertilized oocytes had been continuously cultured in modifed CZB medium. 100%, 100% and 97.9% of 1-cell embryos developed to 2-cell stage in sp18 mAb, nonspecific mouse IgG and non-IgG group 30 h after the start of fertilization, respectively. In the nonspecific mouse IgG and non-IgG groups, 64.1 % and 64.3% of embryos developed to the 4-cell stage, respectively, but all developing eggs in sp18 mAb groups arrested development in vitro at 2-cell stage. After zonae of 2-cell blocked embryos were enzy-matically removed with 0.5% pronase, detection of sp18 antigens by IIF indicated that the fluorescence scattered on two embryonic cells. For embryos fertilized in vivo and co-cultured with 182 μg/mL sp18 mAb, the numbers of 1-cell embryos reaching the 2-cell and 4-cell stage were 95. 2% and 70. 5%, which were not significantly (P>0.05) different from the control group (92.9% and 77.9%). These results indicate that the sp18 antigens on posterior head of mouse sperm were incorporated into the egg plasma membrane during fertilization, and played an active role in development of murine preimplanta-tion embryo.     

菜心多倍体诱变及其细胞学观察

报道了用秋水仙素水溶液处理菜心种子及幼苗生长点获得多倍体的方法,并对获得的四倍体与二倍体（ＣＫ）进行了形态学、细胞学以及结籽率等的比较观察。试验结果表明：（１）在１０～１５℃的条件下,用０．１％秋水仙素水溶液处理幼苗生长点４８小时的诱变效果最好,诱变率可达２６．６７％,浸泡种子效果不佳,最高诱变率仅为４．０％。（２）对四倍体植株分别进行人工自交均获得了种子,但结籽率较二倍体（ＣＫ）明显降低,平均达４２．３％,而八倍体植株几乎是完全不育的。（３）与二倍体（ＣＫ）比较,四倍体植株生长旺盛,花薹的产量和品质均有所提高。（４）随着染色体组的增加,花粉粒大小和叶片保卫细胞中的叶绿体数均显著增加,可作为鉴定植株倍性的一个指标     

基于Web的远程教育课程表的设计与实现

主要介绍了一个基于Ｗｅｂ的远程教育课程表的功能设计与实现细节·重点阐述了使用Ｊａｖａ语言进行网络通信与交互控制的技术方法·使用了ＪａｖａＪＤＫ１－０２开发环境·其中,日历的绘制可根据设置方法中的相应参数来适应不同的月份;它通过ＵＲＬ读取数据文件,而不依赖于具体的主机;对数据的修改可通过浏览器,调用ＣＧＩ程序完成,充分发挥了Ｊａｖａ的网络编程优势,并具有良好的可移植性·     

广义SRLW方程的Fourier谱方法

考虑了一类广义的对称正则长波方程的周期初值问题。对所论问题构造了不同于已有文献的半离散和全离散的Ｆｏｕｒｉｅｒ－Ｇａｌｅｒｋｉｎ谱格式,采用更精细的估计不等式,给出了近似解的收敛性证明和最佳误差估计,改进了已有文献的结果。     

黄精凝集素Ⅱ对大鼠主动脉Ca^2＋跨膜流动的影响

用４５Ｃａ跨膜流动技术测定黄精凝集素Ⅱ对大鼠主动脉Ｃａ２＋跨膜内流的影响．黄精凝集素Ⅱ在０．１～１０μｍｏｌＬ－１时,不影响静息状态下Ｃａ２＋的内流,但能抑制ＮＥ和ＫＣｌ引起的Ｃａ２＋内流,其抑制能力依赖于凝集素的浓度;在１０μｍｏｌＬ－１时,可完全阻滞ＮＥ引起的Ｃａ２＋增加,并能有效地阻滞ＫＣｌ引起的内流作用．表明黄精凝集素Ⅱ能阻滞细胞外的Ｃａ２＋通过受体操纵钙通道（ＲＯＣ）和电压依赖钙通道（ＰＤＣ）的内流     

新集二矿选煤厂使用动筛跳汰机工艺流程的设计

介绍了新集二矿选煤厂使用动筛跳汰机工艺流程的设计特点及实际应用效果     

破坏准则的描述新方法

提出了一种描述破坏准则的新方法。它特别适合对混凝土破坏准则的描述。各种应力组合只能在一个有限的平行四边形区域上变化。采用这种描述方法,能够指导试验数据的选取和破坏曲面的拟合。     

中低Z元素等离子体平均电离度和辐射不透明度的近似计算

在物质处于局域热力学平衡(LTE)的条件下,采用R.M.More提出的改进型平均原子模型和类氢离子的光吸收系数,计算了中低Z元素等离子体的平均电离度及辐射不透明度.将计算结果与SESAME数据库数据和实验数据比较,符合得较好.为便于在辐射磁流体力学计算中的应用,在0.5×106K≤Te≤200×106K的温度范围、10-5(g/cm3)≤ρ≤1(g/cm3)的密度范围对计算结果进行数据拟合,得到了平均电离度和辐射不透明度随温度和密度变化的拟合函数.通过对辐射不透明度采用分温度段拟合,发现拟合值更接近于计算值.     

基于无线传感器网络和GPRS的温湿度远程监测系统

本文针对现有温湿度监测系统的缺陷和不足,采用基于ZigBee的无线传感器网络和GPRS技术,设计实现了一种真正能进行远程环境温湿度监测的系统。本方案充分利用现有的ZigBee和GPRS模块化设备进行组网,编写了ZigBee协调器与GPRS模块的接口程序,并实现了上位机监测软件。结果表明,系统运行稳定、采集温湿度数据准确、部署方便灵活。     

基于四连杆机构的起落架缓冲器行程测量方法

针对小型飞机起落架缓冲支柱行程测试提出了一种测量方法。利用四连杆机构将位移变量转化为角度变量,通过标定试验得到角位移和行程的对应关系;再将实测得到的角位移代入标定方程,即得到实测的缓冲支柱压缩行程。经验证,该方法可以得到满足精度要求的结果;并已成功应用于某型飞机起落架缓冲支柱压缩行程的实测试验。