Checkpoint kinase 1 in DNA damage response and cell cycle regulation

Originally identified as a mediator of DNA damage response (DDR), checkpoint kinase 1 (Chk1) has a broader role in checkpoint activation in DDR and normal cell cycle regulation. Chk1 activation involves phosphorylation at conserved sites. However, recent work has identified a splice variant of Chk1, which may regulate Chk1 in both DDR and normal cell cycle via molecular interaction. Upon activation, Chk1 phosphorylates a variety of substrate proteins, resulting in the activation of DNA damage checkpoints, cell cycle arrest, DNA repair, and/or cell death. Chk1 and its related signaling may be an effective therapeutic target in diseases such as cancer.     

A genome-wide association study in Chinese men identifies three risk loci for non-obstructive azoospermia

Non-obstructive azoospermia (NOA) is one of the most severe forms of male infertility. Its pathophysiology is largely unknown, and few genetic influences have been defined. To identify common variants contributing to NOA in Han Chinese men, we performed a three-stage genome-wide association study of 2,927 individuals with NOA and 5,734 controls. The combined analyses identified significant (P < 5.0 × 10(-8)) associations between NOA risk and common variants near PRMT6 (rs12097821 at 1p13.3: odds ratio (OR) = 1.25, P = 5.7 × 10(-10)), PEX10 (rs2477686 at 1p36.32: OR = 1.39, P = 5.7 × 10(-12)) and SOX5 (rs10842262 at 12p12.1: OR = 1.23, P = 2.3 × 10(-9)). These findings implicate genetic variants at 1p13.3, 1p36.32 and 12p12.1 in the etiology of NOA in Han Chinese men.     

Frequent mutations of chromatin remodeling genes in transitional cell carcinoma of the bladder

Transitional cell carcinoma (TCC) is the most common type of bladder cancer. Here we sequenced the exomes of nine individuals with TCC and screened all the somatically mutated genes in a prevalence set of 88 additional individuals with TCC with different tumor stages and grades. In our study, we discovered a variety of genes previously unknown to be mutated in TCC. Notably, we identified genetic aberrations of the chromatin remodeling genes (UTX, MLL-MLL3, CREBBP-EP300, NCOR1, ARID1A and CHD6) in 59% of our 97 subjects with TCC. Of these genes, we showed UTX to be altered substantially more frequently in tumors of low stages and grades, highlighting its potential role in the classification and diagnosis of bladder cancer. Our results provide an overview of the genetic basis of TCC and suggest that aberration of chromatin regulation might be a hallmark of bladder cancer.     

Behavior Model Construction for Client Side of Modern Web Applications

Most of the behavior models with respect to Web applications focus on sequencing of events,without regard for the changes of parameters or elements and the relationship between trigger conditions of events and Web pages.As a result,these models are not sufficient to effectively represent the dynamic behavior of the Web2.0 application.Therefore,in this paper,to appropriately describe the dynamic behavior of the client side of Web applications,we define a novel Client-side Behavior Model(CBM) for Web applications and present a user behavior trace-based modeling method to automatically generate and optimize CBMs.To verify the effectiveness of our method,we conduct a series of experiments on six Web applications according to three types of user behavior traces.The experimental results show that our modeling method can construct CBMs automatically and effectively,and the CBMs built are more precise to represent the dynamic behavior of Web applications.     

针对要点防空模型的作战兵力优化研究

为了在现代化联合防空作战中,根据蓝方火力配置,红方能够做出最优的兵力分配,使防空效能最大化。从要点防空的任务角度出发,对作战兵力分配进行建模优化,并对优化后的兵力分配效能进行评估。首先选定任务背景为近海小规模局部战争,对复杂作战情况进行抽象得到模型。对模型进行优化,求解得到红方最优兵力分配结果,提出一种新方法,简化了对有限制的类二项分布期望的求解,得到蓝方各类进攻力量成功突防的期望以及红方要点平均损耗期望,并用此值定量评估联合防空的兵力分配效能。最后,进行了实例分析,验证了模型的有效性。     

环境标准的现状与作用分析

介绍了我国环境标准的基本现状,对我国环境标准的制定目的、作用作了阐述,指出环境标准对环境保护的重要性.     

阶段原型法及其支持环境

本文介绍了阶段原型法(SPA)及其支持环境(PEMIS)的总体结构和主要功能,探讨了知识库和推理机制在循环控制中的作用,以及软件重用技术、图形接口技术和应用生成技术等实现技术。     

Immune biology of Ag-specific γδ T cells in infections

Accumulating evidence suggests that human γδ T cells act as non-classical T cells and contribute to both innate and adaptive immune responses in infections. Vγ2 Vδ2 T (also termed Vγ9 Vδ2 T) cells exist only in primates, and in humans represent a dominant circulating γδ T-cell subset. Primate Vγ2 Vδ2 T cells are the only γδ T cell subset capable of recognizing microbial phosphoantigen. Since nonhuman primate Vγ2 Vδ2 T cells resemble their human counterparts, in-depth studies have been undertaken in macaques to understand the biology and function of human Vγ2 Vδ2 T cells. This article reviews the recent progress for immune biology of Vγ2 Vδ2 T cells in infections.     

中国人看生物技术——2003-2007研究报告

本文基于中国公众对生物技术应用态度的研究,项目从2003年开始到2007年结束,先后获得中国和丹麦政府间科技合作项目、浙江省自然科学基金、浙江省科技国际合作项目的资助.研究参照欧洲EUROBAROMETER研究范式设计问卷,采用职业分层抽样的方法,对我国代表性样本浙江四个地区的4157名公众进行了生物技术应用态度的纵向实证研究,定量分析并定性描述了我国公众对生物技术应用态度的轮廓和远景.