拟步甲昆虫基因组DNA提取的比较研究

对拟步甲科8种昆虫的新鲜标本及无水乙醇浸泡标本，分别用SDS-蛋白酶K消化法和乙酸钾抽提法进行基因组总DNA的提取．经5次重复实验，结果表明，用两种方法均能从新鲜标本中获得较完整的DNA，且DNA得率较高；而对于无水乙醇保存的标本，提取出的DNA用琼脂糖凝胶电泳检测不出DNA条带，说明标本在保存过程中DNA可能发生了变化，致使提取出的DNA量太少或未分离出DNA．通过对比实验，分析原因，阐明了两种方法的优缺点，并提出了该类昆虫用于DNA提取的标本的保存方法．     

四川省蝗虫一新属及一新种(直翅目:蝗总科)

记述1992年在四川省凉山地区进行蝗虫调查时发现的蝗虫新属彝蝗属Yiacris及新种蓝翅彝蝗Yiacris cyaniptera。     

峨眉腹露蝗精子的超微结构

通过对峨眉腹露蝗 Fruhsforferiola omei(Rehn et Rehn)精子的透射电镜观察,发现其中心粒侧体呈片状结构,线粒体衍生体纵脊数目为3～4个,精子形成过程中轴丝及线粒体衍生体周围有一定数目的微管分布,这些特点构成了与其它蝗虫精子超微结构相区别的依据.     

黄条黑纹蝗（直翅目：蝗总科）雄性的发现及染色体C带核型分析

报道了在云南西双版纳州勐腊县进行蝗虫调查时采到的黄条黑纹蝗（ＭｅｌｔｒｉｐａｔａｃｈｌｏｒｏｎｅｍａＺｈｅｎｇ）雄性个体，并对其形态结构进行了描述，同时分析了该种的染色体Ｃ带核型．     

中国螽斯总科二新种（直翅目：螽斯总科）

报道了螽斯总科蛩螽科一新种，缙云隐翅螽Ａｃｙｒｔａｓｐｉｓｊｉｎｙｕｎｅｎｓｉｓｓｐ．ｎｏｖ；螽斯科一新种，黄山寰螽Ａｔｌａｎｔｉｃｕｓｈｕａｎｇｓｈａｎｅｎｓｉｓｓｐ．ｎｏｖ．模式标本均保存于陕西师范大学动物研究所．     

中国刺翼蚱科—新属三新种记述（直翅目：蚱总科）

记述了来自浙江、福建、安徽及广西等地区刺翼蚱科—新属三新种，即佯鳄蚱属，新属Ｐａｒａｇａｖｉａｌｉｄｉｕｍｇｅｎ．ｎｏｖ，弯刺佯鳄蚱，新种Ｐ．ｃｕｒｖｉｓｐｉｎｕｍｓｐ．ｎｏｖ，三齿佯鳄蚱，新种Ｐ．ｔｒｉｄｅｎｔａｔｕｍｓｐ．ｎｏｖ及直刺佯鳄蚱，新种Ｐ．ｏｒｔｈａｃａｎｕｍｓｐ．ｎｏｖ．     

陕西省蜻蜒目的初步调查

本文系统调查了陕西省蜻蜓目昆虫的种类和分布,共记载蜻蜓目昆虫63种,分属于2亚目11科34属,其中有2种国内新纪录,22种陕西省新纪录及2新种.     

广西剑螽属—新种（直翅目：蛩螽科）

记述蛩螽科剑螽属昆虫一新种，即凹尾剑螽Ｘｉｐｈｉｄｉｏｐｓｉｓ　ｌａｃｕｓｉｃｅｒｃａ　ｓｐ．ｎｏｖ．标本采自广西环江地区。模式标本存陕西师范大学动物研究所。     

湖南省蚱总科的调查(直翅目)

系统报道湖南省蚱总科名录并3新种,即壶瓶山玛蚱Mazarredia hupingshanensis sp.nov.、湖南玛蚱Mazarredia hunanensis sp.nov.及壶瓶山无翅蚱Aalatettix hupingshanensis sp.nov.壶瓶山玛蚱近似于凸背玛蚱M.convexa Deng,zhengWei,2007,主要区別为:1)颜面隆起在触角之间部分的宽度与触角基节等宽;2)侧面观背板上缘微波状;3)后突到达后足股节顶端;4)中足股节下缘平直;5)后足股节下侧外面非黑色;6)后足胫节褐色.湖南玛蚱近似于平背玛蚱M.platynota Zheng et Ou,2010,主要区別为:1)侧面观头顶与颜面隆起形成钝圆形;2)侧面观颜面隆起在侧单眼前略凹陷;3)侧单眼位于复眼前缘的中部;4)侧面观背板上缘在肩部前略波状,在肩部后平直;5)中足股节宽小于前翅宽;6)后足股节下侧外面非黑色;7)后足胫节褐色.壶瓶山无翅蚱蚱近似于乐山无翅蚱Aalatettix leshanensis Zheng,Caochen,2011及苍山无翅蚱A.cangshanensis Zheng,LinZhang,2013,其区别于两者为:1)侧面观头顶与颜面隆起形成圆形;2)侧面观前胸背板上缘近平直;3)后突到达后足股节膝部.该新种又区别于苍山无翅蚱为:1)头顶突出于复眼之前;2)中足股节下缘波状.该新种又区别于乐山无翅蚱为颜面隆起在触角之间部分的宽度略大于触角基节宽.     

Methane oxidation by an extremely acidophilic bacterium of the phylum Verrucomicrobia

Aerobic methanotrophic bacteria consume methane as it diffuses away from methanogenic zones of soil and sediment. They act as a biofilter to reduce methane emissions to the atmosphere, and they are therefore targets in strategies to combat global climate change. No cultured methanotroph grows optimally below pH 5, but some environments with active methane cycles are very acidic. Here we describe an extremely acidophilic methanotroph that grows optimally at pH 2.0-2.5. Unlike the known methanotrophs, it does not belong to the phylum Proteobacteria but rather to the Verrucomicrobia, a widespread and diverse bacterial phylum that primarily comprises uncultivated species with unknown genotypes. Analysis of its draft genome detected genes encoding particulate methane monooxygenase that were homologous to genes found in methanotrophic proteobacteria. However, known genetic modules for methanol and formaldehyde oxidation were incomplete or missing, suggesting that the bacterium uses some novel methylotrophic pathways. Phylogenetic analysis of its three pmoA genes (encoding a subunit of particulate methane monooxygenase) placed them into a distinct cluster from proteobacterial homologues. This indicates an ancient divergence of Verrucomicrobia and Proteobacteria methanotrophs rather than a recent horizontal gene transfer of methanotrophic ability. The findings show that methanotrophy in the Bacteria is more taxonomically, ecologically and genetically diverse than previously thought, and that previous studies have failed to assess the full diversity of methanotrophs in acidic environments.