重型车荷载作用的公路斜拉桥振动响应分析

为了分析重型车荷载对高速公路大跨度斜拉桥振动响应的影响,以一辆六轴重型车和鄱阳湖二桥为研究对象,考虑车重和车速的随机性,基于车桥耦合振动理论,按照最不利荷载作用位置,采用Newmark-β数值方法求解重型车在行车道偏载行驶时的斜拉桥振动响应。研究结果表明：全桥结构的横向刚度大于竖向刚度。斜拉桥振动响应最大值与车重的随机性有很大关系,两者的概率分布特征相同,而车速的随机性对斜拉桥振动响应最大值的影响并不明显。纵梁的竖向振动响应最大,纵向振动响应次之,而横向振动响应最小,竖向振动响应最大值超过90 mm的概率为1.7%。桥塔的纵向振动响应大于横向和竖向振动响应,纵向振动响应最大值超过30 mm的概率为1.2%。斜拉索面内振动响应大于轴向振动响应,而面外振动响应最小;各拉索轴向振动响应随着拉索长度的增加而增大,面外振动响应因拉索长度不同而各异,主跨较长拉索的面内振动响应幅值相差较大,其他拉索的面内振动响应幅值相差较小;最长拉索的轴向、面外、面内振动响应最大值分别分别超过32 mm、2 mm、40 mm的概率为1.7%、2.4%、0.4%。     

基于知识图谱的核电设备健康管理知识建模与分析

随着信息技术发展,设备健康数据与知识图谱技术结合为设备健康发展带来新的发展机遇,知识图谱利用其多种特异性优势,使企业系统中积累的海量设备维修数据得到有效整合利用.本文提出基于知识图谱的核电设备健康管理知识建模与分析方法,并根据实体关系模型构建维修知识图谱本体框架,同时基于核电企业实际的维修工单数据,从统计分析和关联分析两方面取得良好的实践效果.研究结果表明,知识图谱能够在设备维修知识的集成上解决传统的数据孤岛问题,同时能基于故障的可视化分析为维修人员和管理人员提供决策和知识支持.     

人源程序性死亡配体PD-L2蛋白的原核表达与抗血清制备

利用原核表达系统获得包涵体表达形式的重组蛋白h PD-L220~123(Ig V结构域)和h PD-L220~208(Ig V结构域和Ig C结构域);将重组蛋白利用Ni-NTA柱亲和层析纯化与复性后,作为免疫原免疫Balb/c雌性小鼠制备鼠抗人的多抗血清,经ELISA法检测其效价均达到1∶106;Western-blot实验结果表明,制备的多抗血清均能特异性识别h PD-L2蛋白.以制备的多抗血清进行IHC实验评估与鉴定,结果显示:以h PD-L220~123重组蛋白为免疫原制备的多抗血清阳性较弱且定位模糊,以h PD-L220~208重组蛋白为免疫原制备的多抗血清具有很强的膜定位且背景清晰.     

牛肉及其制品中肉类掺假的荧光PCR鉴别体系优化

根据可能用于牛肉及其制品掺假的肉类原料(猪肉、鸡肉和鸭肉),设计了4对引物和探针.经过测试其具有良好的特异性后,对影响各自聚合酶链式反应(polymerase chain reaction,PCR)扩增的5个影响因素包括Mg2+浓度、Taq DNA聚合酶活、dNTPs浓度、引物和探针浓度以及模板DNA用量等进行了优化,确定了最佳的PCR扩增体系.同时,根据优化后的结果,对特异性进行进一步的测试,并使用建立的优化荧光PCR鉴别体系对混合样品和市售样品进行检测,确定整体检出限为0.1%(质量分数),并证明该鉴别体系的实际应用价值.     

预测气液两相分层流界面剪切应力的新方法

针对水平管气液两相分层流界面剪切应力的预测,建立了基于计算流体力学（CFD）方法的剪切滑移壁面模型,即将气液界面处理为固定的具有均匀剪切应力的水平滑移壁面。利用FLUENT 软件模拟了气相流动,气液界面采用剪切滑移壁面边界条件,通过对比实验测量的气相流场分布得到气速和界面剪切收敛值,并由收敛值拟合得到界面摩擦因子与气相雷诺数的关联式。应用这一关联式的预测结果与实验间接测量值吻合较好,并且在气、液相雷诺数9 4006ReG650 000 和21 0006ReL630 000 范围内优于Taitel 和Dukler 与Sidi-Ali 和Gatignol 模型。表明剪切滑移壁面模型可以有效地控制关键参数,提高预测效率和精度,为CFD 方法预测气液两相分层流界面剪切应力提供了新的思路。     

Suppressing progress of pancreatitis through selective inhibition of NF-κB activation by using NAC

OBJECTIVE: To explore the characteristics of NF-KappaB activation in the progress of pancreatitis, the relationship with expression of TNF-alpha in the inflammatory reaction, and prevent the exacerbation of pancreatitis by using NAC. METHOD: Forty-eight rats were divided into three groups: therapy (group C), pancreatitis (group B) and control (group A). NAC served as the inhibitor of NF-KappaB activation. In the time intervals of 1.5, 3.0, 6.0, 12.0 hour, NF-KappaB activation was detected with flow cytometry (FCM) and the expression of TNF-alpha mRNA and protein with in situ hybridization (ISH) and enzyme-linked immuno-sorbent assay (ELISA) respectively. Meanwhile, the level of lipase and amylase in the serum was assayed and the pathological change was evaluated. RESULT: NF-KappaB activation in the pancreatitis group was higher than that in the control group (P<0.01), peaked at 3 hours, and was depressed by the inhibitor of NF-KappaB, NAC. The expression of TNF-alpha as well as the level of lipase and amylase in the serum also rose synchronously with activation of NF-KappaB. In contrast to group A, it was significantly different (P<0.01) in group B. After using NAC in group C, all of these values were decreased and the inflammatory reaction in the pancreas abated evidently. The pathology changes of the pancreas were shown to be alleviated in group C. CONCLUSION: First, NF-KappaB activity is intensively initiated in the course of pancreatitis and shown to have closely relationship with the release of cytokines. Second, use of NAC markedly depressed NF-KappaB activation. TNF-alpha expression is down regulated by cytokines. It is suggested that NAC probably acts as a useful agent for treatment of pancreatitis by indirectly inhibiting activation of NF-KappaB.     

Correlations for the ignition delay times of hydrogen/air mixtures

Correlations for the ignition delay times of hydrogen/air mixtures were developed using the method of High Dimensional Model Representation (HDMR).The hydrogen/air ignition delay times for initial conditions over a wide range of temperatures from 800 to 1600 K,pressures from 0.1 to 100 atm,and equivalence ratios from 0.2 to 10 were first calculated utilizing the full chemical mechanism.Correlations were then developed based on these ignition delay times.Two forms of correlations were constructed:the first one is an overall general model covering the whole range of the initial conditions;while the second one is a piecewise correlation model valid for initial conditions within different sub-domains.The performance of these correlations was studied through comparison with results from the full chemical mechanism as well as experimental data.It was shown that these correlations work well over the whole range of initial conditions and that the accuracy can be significantly improved by using different piecewise correlations for different sub-domains.Therefore,piecewise correlations can be used as an effective replacement for the full mechanism when the prediction of chemical time scale is needed in certain combustion modeling.     

高温后横向压力对带肋钢筋与高强混凝土间粘结的影响

配制C100高强混凝土,分别浇筑成混凝土立方体抗压试块和特制的黏结试块.对立方体试块和黏结试块进行200,300,400,500,600℃的高温试验,测量不同温度经历下高强混凝土的残余抗压强度和基于损伤的黏结强度,并分析横压力与黏结强度的关系.对高温后高强混凝土的表观特征和带肋钢筋与高强混凝土黏结强度的退化机理进行研究,为火灾后高强混凝土结构鉴定及加固提供技术支持和理论依据.     

国家免疫第二道防线：经济系统防御

经济系统构成国家免疫系统的第二道防线,经济系统防御能力和健康程度取决于一个国家的经济规模、经济水平、经济增长、真实储蓄、国家信用、开放程度和资源安全等关键因子。本文对45个样本国家的经济防御能力进行了定量评估与分析,研究表明：有3个国家属于经济防御能力“较强国家”,分别是中国、印度和美国;有14个国家属于经济防御能力“一般国家”;有19个国家属于“较差国家”;有9个国家属于经济防御能力“脆弱国家”。中国国家经济防御能力指数（0．818）在45个国家中排第1位,远远高于第2位的印度（O．770）和第3位的美国（O．675）。强大的经济防御能力,为我国成功抗击百年不遇的全球金融危机,实现健康可持续发展,提供了基础保障。     

L-半胱氨酸修饰的水溶性掺杂型CdZnTe量子点的水热法制备和表征

通过水热法,以Zn离子作为掺杂材料,L-半胱氨酸为稳定剂,制备出了水溶性的掺杂型CdZnTe量子点.应用高温高压的水热合成法,有效地减少了量子点表面的缺陷,过渡金属Zn的掺杂有效降低了CdTe量子点的毒性,结果表明:制备出的CdZnTe量子点含有高比例掺杂剂,性质稳定、单分散性好、量子产率高,具有良好的生物相容性,可广泛应用于生化检测和生物医学研究.