Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization

During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AAF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8. A mixture of cosmid cHT2-derived subclones of the 3' part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated.     

基于DSP的电子耳蜗语音处理器的研制

电子耳蜗是一项正在迅速发展的聋人康复新技术，其关键是要研制出可靠、灵活、价格低廉的产品。首先介绍电子耳蜗的原理及其进展，然后介绍基于TMS320VC5402 DSP处理器的电子耳蜗语音处理器。实验证明：基于DSP的电子耳蜗系统可以按照患者的实际情况灵活选择各种语音处理方案，并能方便实现语音预处理、噪声抑制和对应各电极的频谱阈值调整，是一种比较好的系统结构。     

7—氯—8—甲基喹啉氯化过程研究

本文用ＨＭＯ法对７－氯－８－甲基喹啉的电子密度、键级和自由价进行了计算，认为７－氯－８－甲基喹啉可以进行Ｃ３位亲电氯代反应。通过实验研究氯化过程，测定出六种氯化物，结果表明，在合适条件下，Ｃ３位的氯化伴随着甲基侧链的自由基氯代反应同时发生。     

板材辊式矫直机辊数研究

本文根据弹塑性弯曲理论，应用迭代法计算了板材一次弯曲矫直时的弯曲力矩和相应的反弯曲率，计算给定方案下形成的残余曲率，给出了压下量与反弯曲率的数学表达式．对于一定的矫直精度要求，提出了上排工作辊集体倾斜调整的辊式矫直机辊数的计算方法．     

外部收益率讨论

着重讨论了外部收益率在投资方案中的选择和比较问题，证实了外部收益率的实用性。     

平面桁架结构损伤的探测、定位与评估

利用参数识别技术描述了对平面桁架结构损伤的探测、定位与评估（ＤＬＥ）的一个新算法。这一概念始于精确＂健康＂模型，利用动力响应数据周期地更新这一＂健康＂模型，当损伤发生时，用一个识别算法（一个显式桁架损伤模型），以动力响应数据构造出一个桁架损伤模型，由此比较损伤模型与健康模型的参数，从而探测、定位及评估损伤的程度     

水火弯板几个成形参数的实验分析

提出船体外板水火加工影响成形因素的测试方法。通过对船厂实船板加工数据的测量，定性分析温度应力、加热线顺序、加工板曲率、边界条件及二次边热对成型效果的影响规律，并给出其变化曲线和数据对比。探讨了影响变形的本质。为理论分析奠定实验基础。     

Rapid-time-course miniature and evoked excitatory currents at cerebellar synapses in situ.

Neurotransmission from mossy fibre terminals onto cerebellar granule cells is almost certainly mediated by L-glutamate. By taking advantage of the small soma size, limited number of processes and short dendrite length of granule cells, we have obtained high-resolution recordings of spontaneous miniature excitatory postsynaptic currents (m.e.p.s.cs) and evoked currents in thin cerebellar slices. Miniature currents have a similar time-course and pharmacology to evoked currents and consist of an exceptionally fast non-NMDA (N-methyl-D-aspartate) component (measured rise-time, 200 microseconds; estimated pre-filtered rise-time less than 100 microseconds; decay time constant, tau = 1.0 ms), followed by 50 pS NMDA channel openings that are directly resolvable. We could find no evidence for the recent proposal that miniature currents in granule cells are mediated solely by NMDA channels with a novel time course. The non-NMDA receptor component of m.e.p.s.cs has a skewed amplitude distribution, which suggests potential complications for quantal analysis. The difference in time course between the m.e.p.s.cs reported here and other synaptic currents in the brain could reflect differences in synaptic function or electrotonic filtering; the relative contribution of these possibilities has yet to be established.     

实验性脾虚证大鼠血清中微量元素含量的研究

用破气苦降药加饮食失节法复制了大鼠脾虚证模型。采用原子吸收光谱法检测了血清发Ｚｎ、Ｃｕ和Ｆｅ的含量。本研究证明，脾虚证大鼠血清Ｚｎ值明显降低（Ｐ＜０．００１）；Ｃｕ值、Ｆｅ值无明显差异（Ｐ＞００５）；Ｃｕ／Ｚｎ比值上升。用中药加味四君子汤治疗后，血清Ｚｎ值明显回升并高于正常水平（Ｐ＜００５），Ｃｕ值回升（Ｐ＜０．０５），但Ｆｅ值和Ｃｕ／Ｚｎ比值与自然恢复及对照组比较无显著性差异。这说明：１．脾虚组Ｚｎ的降低可能与消化吸收障碍有关；２．加味四君子汤对脾虚证时血清Ｚｎ及Ｃｕ／Ｚｎ比值的变化有明显的调整和恢复作用．     

认识银河系400年

银河和银河系是两个完全不同的概念。古人早就知道夜间天空中有一条银河．而只是从17世纪初起．天文学家才认识到银河系的存在。人类对银河系结构和运动的认识已经历了约400年的漫长时间。有关银河系的一些重大观测和理论问题一直是天文学和天体物理学中极为重要的研究领域。