Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

Development of Full Automatic External Welding Machine for Annular Welds of Pipeline

To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided.     

制备介孔复合环境材料的酸洗刻蚀工艺

探索了一种制备介孔复合环境材料的方法--破坏式造孔与有机复合相结合,使最终产品可大量吸附污染水体中的各种污染物并具有一定的杀菌功能.该方法通过酸洗刻蚀,达到了去除原材料中的杂质和刻蚀孔径的目的,所制备的中间产品24h吸湿率为原材料的2倍,染料吸附量为原材料的1.6倍.     

极低硫钢的精炼脱硫动力学模型

通过对影响脱硫反应限制性环节的分析,将持续接触和瞬时接触两种反应模式综合考虑,建立了精炼脱硫动力学模型,并通过实验室"极低硫钢精炼脱硫"实验数据对模型进行验证.结果表明,本模型的预测结果与实验结果比较吻合.     

峰谷电价体制下东北输油管网日输油优化研究

东北原油长输管网是中国规模最大的地区性原油运输系统，就该管网基于峰谷电价体制下的运行现状，建立了线性规划数学模型，提出了日输油计划优化问题，并以铁秦线为例进行分析；初步揭示了峰谷电价体制下日输油计划方式的一些基本规律， 表明在该体制下东北管网具有一定的节能降耗潜力。但该方式在一定程度上受到管道本身运行特性的约束，与热油管道相比它更适合于等温管道。     

Spectroscopic study on the photophysical properties of chlorine substituted tetraphenylporphyrin-histidine and its zinc(Ⅱ)complexes

As the structural analogues of (bacterio)chlorophylls in photosynthetic organisms, porphyrins play an important role in building up artificial photosynthetic systems[13]. In natural photosynthetic pigment-protein complexes,certain amino acid residuals modulate the reactions ofcharge transfer and energy transfer via coordination andother types of interactions with chlorophyll molecules.For instance, in higher-plant photosynthetic reaction cen-ter PSⅡ, the oxidization-reduction system consistin…     

筒型基础系缆平台沉／拔过程侧摩阻力原型测试

介绍了1999年首次安装在渤海锦州9－3地区多筒基础平台安装施工过程中的侧摩阻力原型测试研究，描述了简型基础上沉和上拔过程中筒壁侧摩阻力的测试系统和测试方法，给出了主要测试结果并与现行规范进行了对比分析，这项成果对筒型基础平台的设计和施工具有直接的参考意义。     

我国中小企业现状及竞争前景

阐述并分析了我国中小型企业在发展中存在的问题。根据中小型企业自身的特点，提出中小型企业可以利用自身优势进行横向联合发展，并处理好成本、特色经营、产品创新之间的关系；树立风险意识，积极创新，扩大多渠道的融资等竞争策略，以适应WTO的全球经济化的需求。     

甲醇催化脱氢制甲酸甲酯反应中Cu－ZnO－ZrO_2／SiO_2催化剂的特性

Ｃｕ－ＺｎＯ－ＺｒＯ２／ＳｉＯ２催化剂对甲醇催化脱氢制甲酸甲酯的反应具有良好的活性和选择性．应用连续微反、差示热分析、Ｘ射线衍射、程序升温还原、Ｘ射线光电子能谱分析和红外光谱等技术，对该催化剂的活性中心本质、ＺｎＯ及ＺｒＯ２的助催化作用、可能的反应机理以及催化剂的失活原因进行了探讨．     

层次分析法在城市土地分级中的应用

探讨了用层次分析法对城市土地等级进行定量评价的原理和方法，并以济南市为例进行实证研究，取得了较好的效果，为土地有偿使用和城市规划提供了依据。