Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

基于线性经济模式批判的服装网络营销渠道分析

从经济学角度来讲，线性经济模式把自然环境当做是为了实现自身利益而自由开采和利用的资源，使得我们对自己的经济决策所造成的环境后果不需要负责。越来越多的企业开始转变生产和营销模式，本文在批判线性经济模式的基础上分析了服装企业网络营销的渠道建设，并建立了相关的生态链。     

高水平大学生足球运动员运动损伤的调查与分析

通过对参加2004-2005飞利浦中国大学生足球联赛南区决赛的156名高水平大学生足球运动员进行调查研究,对其运动损伤的特征、易发部位及原因进行了分析,并提出了相应的预防措施,以增强大学生足球运动员的防伤病意识,从而更好的促进高校足球运动的发展。     

浅析电机节能降耗改进方法

文章介绍了几种对矿山机电设备常用电机进行技术改造的方法，实现了节能降耗，降低成本的目的，对企业具有一定的经济价值。     

INFLUENCE OF POLYMER CHARACTERISTICS AND MELT-SPINNING CONDITIONS ON THE PRODUCTION OF HIGH RANDOMNESS 60PHB/PET THERMOTROPIC LIQUID CRYSTAL COPOLYESTER FIBER

Six samples of linear high randomness 60PHB/ PET thermotropic liquid crystal copolyesters are made by melt copolymerization at 290℃ , whose randomness about 0.955 is measured by the discernible 'H-NMR spectrometer. High tenacity, high module fiber is prepared by melt spinning in liquid crystal phase. The effect of molecular weight, shear rate, temperature as well as spinning drawn ratio on the mechanical behavior of 60PHB / PET copolyester fiber are shown that, lower shear rate (2~ 10 s~(-1)), higher temperature melting (300℃ ), lower temperature spinning (280℃ ) and higher molecular weight are favourable to the increase of the fiber mechanical properties. With the variance of drawn ratio, fiber mechanical property has a transition point due to traversion from shear-orientation to drawn-orientation. The copolyester fiber has high crystallinity, high orientation at the crystalline region, high chain orientation and high regular fibrillar structure.     

一类递推关系的计算定理及其应用

论述了关于二次型递推关系的一类问题，并提出一种新的计算定理和相应的计算方法。     

中长跑的选材与预测

本文从系统学角度,探讨如何控制中长跑选材的过程,并从生理、心理、身体素质以及身体形态等方面探讨如何以科学的选材方法和手段为依据,指导教练选出优秀人材,进行早期培养,缩短其成材周期,提高成材率。     

固体表面化学发光基质背景降低的研究

对固体表面化学发光法固体基质的发光背景进行了考察．提出了降低背景的方法，以ＮａＯＨ与水的质量比值为１．５％溶液、１０－２ｍｏｌ·Ｌ－１ＥＤＴＡ溶液或其混合溶液浸泡滤纸２４ｈ，滤纸背景的降低分别为６０％，７９％及８２％．     

甘肃产马先蒿属植物分种检索表

本文对甘肃产马先蒿属５２种、７亚种、６变种和１变型列了分种检索表，并在括号内记载了产地，为开发该属资源提供依据。     

蔸兰立枯病原菌及颉抗菌防治的研究

本文对１３种蔸兰，２４９个发病器官进行了病原真菌的分离，共获得真菌菌株１９２个，其中立枯丝核菌（Ｒｈｉｚｏｃｌｏｎｉａｓｏｌａｎｉｋｕｈｎ）有１１８个菌株，占整个真菌菌株的６１．４５％，用分离的立枯丝核菌ＹＫ－２６２，菌株对同色蔸兰等４种蔸兰进行了回接试验，共接４２株１３８片叶，发病叶１１２片，发病率平均８８．９％，而对照为８．３％．证明立枯丝核菌是该病的病原菌。用菌丝融合法测试，确定是一个新的菌丝融合群，按国际编号定为ＺＡＧ－８群。绿色木霉菌７８菌株对该病原菌有明显的颉抗作用，初步测定对同色蔸兰立枯丝核菌的防治有效率为８８．８％～９３．３％；对长瓣蔸兰为７７．８％～８１．１％；对带叶蔸兰为７４％．