Recombinant soluble TRAIL induces apoptosis of cancer cells

TRAIL is a tumor necrosis factor family member that selectively induces apoptosis of cancer cells but not of normal cells. To develop TRAIL into a potential cancer drug, three different sizes of soluble TRAIL fragments, including sTRAIL(74—281), sTRAIL(95—281) and sTRAIL(101—281), were expressed in E. coli and purified to homogeneity. Apoptosis assays indicated that sTRAIL(95—281) and sTRAIL(101—281), but not sTRAIL(74—281), can potently induce apoptosis of various cancer cell lines in 6 h, suggesting that the N-terminal fragment of aa101 has inhibitory effect on TRAIL-induced apoptosis. Moreover, we found that some cancer cells were resistant to TRAIL and the resistant cells could be converted into sensitive cells by treatment with the protein synthesis inhibitor cycloheximide, suggesting that one or more short-lived proteins are responsible for cells’ resistance to TRAIL.     

Rapid senescence induced by overexpression of p53 in NIH3T3 cells

An NIH3T3 cell line which overexpresses temperature-sensitive p53Val135 was constructed by introduction of p53Val135 gene. It exhibited rapidly characteristic morphological and biochemical alterations related to repli-cative senescence when being cultured in 32℃. We suggested that the overexpression of p53 activated probably the onset of senescence in NIH3T3 cells, which induced a rapid cellular senescence.     

民用建筑安装工程土建与安装配合施工刍议

文章对建筑安装工程施工中土建与安装之间配合存在的问题和原因进行了剖析,并提出相应的对策。     

遥感影像浅水河道提取二维EMD方法

遥感影像中浅水河道与居民地具有相似的光谱特性,在浅水河道自动提取过程中噪声较多,经验模态分解(EMD)可获取原始信号不同尺度上的细节信息,有效地提高遥感影像浅水河道自动提取的精度。利用环境与减灾小卫星数据,以彰武县柳河为研究区,对该区2012年10个时期NDVI时间序列分别EMD分解,选取每个时相信息量较大的前三个固有模态函数(IMF),结合2012年9月18号影像共34波段作为研究数据,利用极大似然法、BP神经网络传统的分类方法进行分类。结果表明结合EMD的分类方法可有效地去除居民地噪声, Kappa系数最高达到0.9690,总体分类精度最高达到93.35%,从而有效地解决了遥感影像中浅水河道提取正确率低的难题。     

50 ku keratin-like protein and β-microtublin coexist in higher plant cells

IF-like proteins have been obtained from suspension cells of Nicotiana tabacum by selective extraction. Western blot analysis shows that the major components of IF-like proteins are 6 keratin-like proteins of 64, 58, 55, 54, 50 and 45 ku. Specially the 50 ku ptotein also reacts with polyantibody against microtublin. Two-dimensional gel electrophoresis shows that the 50 ku protein is composed of two different proteins and their amino acid sequences have been determined. Part of the sequence of one protein is identical to that of β-microtublin and the other protein's sequence has no significant homologue, which should be a new sequence-unknown protein. These results suggest that 50 ku keratin-like protein and β-microtublin coexist in higher plant cells, and that may lead to the phenomenon of co-distribution of IF and microtuble in plant cells.     

一种高效率PS光子互连网络的实验研究

在分析互连元件四脊棱镜和菲涅耳棱镜组等效关系的基础上,对由此构成的光子互连网络进行了实验研究,并给出了具体实验方案及结果。     

会计诚信的若干思考

文章从会计诚信问题形成的原因及如何重塑会计诚信方面作了探索,旨在呼唤会计诚信的回归.     

底水油藏水平井合理位置的确定方法研究

底水油藏中水平井的合理位置是确保水平井高产稳产、延长无水采油期的关键所在。利用镜像反映及势函数的叠加原理,在获得底水油藏中任意一点的势函数后,得到了底水油藏中水平井底势差和水平井的产量方程,由此研究影响水平井产量、临界产量及见水时间的因素,通过研究获得了底水油藏中水平井的合理位置,为水平井开采底水油藏的优化设计提供了理论基础。     

基于多尺度特征融合的超分辨率重建算法研究

超分辨率重建技术可以提高图像质量,使原图像具有更丰富的细节信息。针对现有的超分辨率重建算法存在提取特征单一、不利于对图像信息进一步提取的问题,提出了一种基于多尺度特征融合的超分辨率重建算法。采用多特征提取模块获取更多浅层信息,并在网络中添加密集连接结构,增强特征的传播,减少相关参数计算,减轻梯度消失问题。在Set5和Set14基准数据集上进行了测试,并在电力巡检数据集上进一步验证了算法的有效性。与主流的超分辨率重建方法进行了对比,实验结果表明,该方法生成的图像有更加丰富的细节信息,能够有效地改善图像质量,峰值信噪比与结构相似度值较其他主流算法均有一定的提高。     

建筑设计协同工作模型设计与实践

对建筑设计协同工作的机制进行了研究 ,提出了逻辑模型 ,论述了依据建筑设计在协同工作目标、实施过程和控制机制等若干方面的特点所建立的工作流模型、资源管理模型、虚拟协同设计环境等。在此基础上实现远程建筑设计协同工作通用平台 (TH- CSCDP) ,该系统利用 Auto CAD几何实体句柄的特性 ,设计了面向对象的变更对象模型来管理图纸变更的历史和附加信息并运用计算机网络、数据仓库实现工程级的设计变更管理 ;同时采用网络多媒体技术构造实时虚拟协同设计环境。该系统已经被中国多家设计单位采用 ,实践证明其在管理建筑设计协同资源 ,支持设计资源的共享和传输 ,实现多层次多媒体协同工作环境等方面效用卓著