汽车ABS台架检测方法研究

汽车ABS性能检测是汽车行驶安全性能检测的重要内容,但如何实现在台架上对汽车ABS机械部分性能的检测是需要解决的关键问题.根据ABS工作过程中车轮线速度的变化规律,提出一种ABS台架检测方法,并设计了汽车ABS检测试验台.仿真结果表明,设计的ABS检测试验台能够实现对ABS的检测.     

莪术挥发油亚微乳剂的制备与物理稳定性的研究

目的:研究莪术挥发油亚微乳剂的处方和制备工艺,并对其物理稳定性进行考察.方法:以平均粒径、粒度分布、ζ-电位等参数为指标,采用不同的蒸汽灭菌参数研究确定乳剂的分散相、稳定剂、复合乳化剂等处方组成.结果:所研制的莪术挥发油亚微乳剂载药量高,可耐受热压灭菌,室温下放置6个月基本稳定.其处方为:莪术油2.0%、大豆油2.0%、精制蛋黄磷脂1.2%、油酸0.04%、泊洛沙姆188 0.8%、注射用水加至100 mL.结论:莪术油含有挥发性活性成分,应根据挥发油的挥发特性,设计并制备稳定的可静脉注射的亚微乳剂.     

关于加强创新型人才培养的思考与探索

传统学校教育在教育理念、培养目标、方式以及内容等许多方面都与我国当今倡导的培养创新型人才的教育格格不入。本文对教育改革转型时期创新型人才培养的教育理念、教学思想、培养模式、教学方法与手段、学生个性培养、教师素质及管理体制等若干重要要素进行了思考与探索。     

Generation of transgenic wheat resistant to wheat yellow mosaic virus and identification of gene silence induced by virus infection

The plasmid containing the promoter Act1, the coat protein (cp) gene of wheat yellow mosaic virus (WYMV) and the selectable bar gene, was delivered via particle bombardment, directly into immature embryos of a wheat cultivars. PCR and PCR-RFLP were employed to screen the existence of the cp gene in T0 and T1 generations. Seeds from the positive T1 plants were sowed in fields heavily contaminated with WYMV to detect their resistance. In field trial of virus infection, one of the transgenic wheat lines, P8-T2, exhibited highly disease-resistance. Western blot and RT-PCR analysis showed that the expression level of cp gene in the resistant transgenic line was reduced greatly compared to those susceptible to WYMV infection. This provided evidence to presume that the resistance obtained by the transgenic wheat line was stimulated by the mechanism of the virus induced gene silencing.     

Optical mapping of a rice BAC clone using restriction endonuclease and imaging with fluorescent microscopy at single molecule level

A method of constructing restriction map by optical mapping and single molecule fluorescent microscopy is described. DNA molecules were aligned and adsorbed on a glass coverslip surface by a modified “molecular combing” technique, and then the surface-immobilized DNAs were cleaved in situ with a restriction endonuclease. Individual DNA molecules digested by the endonuclease EcoRⅠ were observable with fluorescent microscopy. Using optical mapping, a physical map of a rice bacterial artificial chromosome clone was constructed. This method will facilitate genomic mapping and tracing the dynamic process in real time at a single molecule level with fluorescence microscopy.     

Nicotiana ovule extracts induce nuclear reconstitution of demembranated Xenopus sperm in cell-free system

Nicotiana tabaccum ovule extracts induced nuclear reconstitution of demembranated Xenopus leavis sperm in a cell-free system. Demembranated Xenopus sperm began to swell after 15 min of incubation with Nicotiana ovule extracts. Accompanying the process of incubation, Xenopus sperm decondensed and their shapes changed gradually from long and ellipse to round. The completely decondensed chromatin was surrounded with membrane structure, which was a mixture envelope of a double membrane and a single membrane. Nucleosome assembly was verified by means of micrococcal nuclease digestion to reconstituted nuclei and DNA electrophoresis. Nicotiana ovule extracts supplied one more experimental model and system. The new system could promote powerfully the research on mechanisms of cell division and cell cycle regulation.     

用高锰酸钠氧化法去除水中的苯胺

研究了高锰酸钠对水中苯胺的去除作用·实验结果表明,随着高锰酸钠投加量的增加,水中苯胺的去除率增加,在苯胺初始质量浓度为10mg/L,温度为18℃,pH值为6 8,反应时间为30min的条件下,高锰酸钠质量浓度为30mg/L时,苯胺的去除率达92 12%·水样的pH值对苯胺去除效果有较大影响,在强酸性及强碱性条件下苯胺去除效果高于中性条件,pH值为3时,苯胺去除率为97 55%;pH值为11时,苯胺去除率为96 33%·升高温度或延长反应时间对苯胺的去除均有正的影响·苯胺是通过高锰酸根和新生态的氧将其氧化成苯醌而去除的·     

PP_(333)对人参果生长发育的影响

通过对人参果苗喷施不同浓度的PP_(333),其结果表明以150ppm的处理最理想,株高比CK降低了29.7％,始果期和盛果期分别提早11天和17天。75ppm和300ppm的处理较CK也有一定的效果,但表现为浓度过低和过高。     

近海污染大气对自由空间量子通信性能的影响

自由空间是量子保密通信的重要应用场景之一。近海地区,大气湿度和各种污染大气气溶胶粒子会导致量子链路性能的衰减。基于Mie散射理论,结合近海地区空气湿度对气溶胶粒径及复折射率的影响,计算吸湿后近海污染大气气溶胶的消光系数。根据气溶胶的粒度谱分布函数,分析近海污染大气气溶胶粒子浓度与星地量子链路衰减、量子链路效率及信道纠缠度之间的关系。针对比特翻转噪声及相位阻尼噪声,研究量子隐形传态保真度的变化。结果表明,近海地区大气相对湿度为80%的环境下,当传输距离为6 km,气溶胶粒子数浓度为60 cm^-3时,对应的链路衰减系数、链路效率、幅值阻尼信道量子纠缠度分别为1.7 dB、2.02×10^-6、0.457。仿真结果可为近海地区量子通信系统的设计与优化提供参考。     

曲线顶管底幕法古沉船整体打捞缩尺模型试验

为了保护沉船的完整性,缩短打捞周期并降低打捞成本,本文提出了一种采用矩形曲线顶管形成底部托盘的新型水下沉船整体打捞方法。基于极限平衡法,推导了单根矩形顶管沿竖曲线推进的顶推力理论计算公式。依托“长江口二号”古沉船打捞项目,开展了缩尺模型试验,分析了底幕不同位置顶管推进顶推力的变化规律,并对一些施工因素对地层扰动及顶推力的影响进行了探讨。结果表明：矩形顶管沿竖曲线推进,顶推力随推进角度的增大呈现先增大后减小的趋势,顶推力峰值发生在推进角度为120°-180°之间;刀盘旋转切削及泥浆的冲刷作用有助于降低对地层的扰动,减小推进阻力;由于端板的约束作用,靠近端板处顶管顶推力大于其他位置的顶管顶推力;顶管在入土和出土时刻会对周围环境产生一定的扰动,因此在确定顶管弧形梁的半径时,需要充分考虑其对沉船扰动的影响;基底吸附力约占总起吊力的31%。本项研究为隧道施工技术在沉船打捞领域的创新性交叉应用,研究成果可为“长江口二号”沉船打捞及类似打捞工程的实施提供重要的理论依据和技术指导。