利用有机溶剂法快速纯化绿色荧光蛋白

介绍非色谱GFP纯化方法——有机溶剂纯化法．该方法在短时间内（30min）即可获得大量较高纯度（91．7％）,且荧光光谱特征不改变的GFP蛋白．纯化过程简单,无需昂贵的仪器和设备,适合绝大多数中小实验室使用．     

肉芝软珊瑚素全合成的研究：Ⅲ.肉芝软珊瑚素的合成

由3,7-二甲基-6,7-环氧-8-苯硫基-2-辛烯醛和6-甲基-8-三甲硅氧基-6-烯-2-酮-1-辛酸乙酯通过羟醛缩合、内酯化、Wittig反应、闭环等反应合成得肉芝软珊瑚素,总产率10.6％。其中的关键步骤是以硫稳定的碳负离子烷基化。讨论了合成品的立体化学。     

Influence of acid leaching and calcination on iron removal of coal kaolin

Calcination and acid leaching of coal kaolin were studied to determine an effective and economical preparation method of calcined kaolin. Thermogravimetric-differential thermal analysis (TG-DTA) and X-ray diffraction (XRD) demonstrated that 900℃ was the suitable temperature for the calcination. Leaching tests showed that hydrochloric acid was more effective for iron dissolution from raw coal kaolin (RCK), whereas oxalic acid was more effective on iron dissolution from calcined coal kaolin (CCK). The iron dissolution from CCK was 28.78wt%, which is far less effective than the 54.86wt% of RCK under their respective optimal conditions. Through analysis by using M?ssbauer spectroscopy, it is detected that nearly all of the structural ferrous ions in RCK were removed by hydrochloric acid. However, iron sites in CCK changed slightly by oxalic acid leaching because nearly all ferrous ions were transformed into ferric species after firing at 900℃. It can be concluded that it is difficult to remove the structural ferric ions and ferric oxides evolved from the structural ferrous ions. Thus, iron removal by acids should be conducted prior to calcination.     

利福定对果蝇寿命的影响

本文研究利福定喂饲果蝇对其寿命的影响。试验组液体饲料分别含利福定10、5和2.5ppm,结果发现这三个浓度的利福定对果蝇均有延寿效应,其中,10ppm的利福定对雄果蝇的延寿效果最好,平均寿命延长22.4％(p<0.05),5ppm的利福定对雌果蝇的延寿效果最好,平均寿命延长19.2％(p<0.05)。而且,它们的最高寿命也有显著延长,提示利福定是一种较好的抗衰老药物。     

考虑固结路径影响的天然沉积土不排水剪切试验研究

针对传统等向固结剪切试验不能反映实际工程中的非等向变形和强度特性的局限,采用GDS应力路径三轴试验仪,对天然沉积结构性软黏土进行了不同固结条件下的不排水剪切试验,探讨不同应力路径对天然沉积土不排水剪切特性的影响.结果表明:相同的平均有效固结应力下土体偏压固结后剪切阶段应力-应变关系曲线的峰值强度大于等向固结的峰值强度;固结过程偏应力的存在提高了土体的总应力强度指标,当控制固结压力终值的剪应力相同时,偏压固结的强度包线相对于等向固结将平行上移;等向固结下当固结压力大于结构屈服压力时,有效应力路径与屈服面具有相似性,剪切屈服破坏后有效应力路径沿着临界状态线下滑;偏应力固结后剪切过程中有效应力路径的走向相对于等向固结发生了向右偏转.     

复合电流滤序器的谐波特性及其对相差高频保护的影响

谐波对电力系统继电保护的影响是电力部门十分关注的问题.本文就稳态谐波对复合电流滤序器和相差高频保护的影响作了计算与分析,并指出谐波对于相差高频保护影响的严重性.文中的计算、分析和结论可供电力系统继电保护工作者在设计、研究和运行分析时参考.     

Simulation of wind gust structure in the atmospheric boundary layer with Lattice Boltzmann Method

Cold fronts occur in northern East Asia during winter and spring.After cold frontal passage,airflow is downward and accompa-nying strong winds fluctuate significantly;this is termed wind gusts.Analysis of observation data shows that wind gust structure has coherent characteristics.This is important for entrainment of spring dust storms into the upper boundary layer,where they are transported great distances.The Lattice Boltzmann Method(LBM) is a computational fluid technique based on the Boltzmann transport equation.The LBM has been used to study complex motion such as turbulence,because it describes motion at the micro level.In this paper,Large eddy simulation is introduced in the LBM,enabling simulation of turbulent flow in the atmospheric boundary layer.The formation and development of wind gusts are simulated,and a coherent structure with a combination of wave and vortex is obtained.This explains the mechanism of soil erosion and sand entrainment by the coherent structure of wind gusts.     

高压除鳞喷嘴内部流场数值模拟与仿真分析

针对目前工厂广泛使用的高压除鳞喷嘴,应用前处理软件Gambit建立其内部流畅的三维模型,采用Fluent软件提供的Laminar层流模型对不同结构参数的喷嘴内部不可压缩、稳态、层流流场进行了数值模拟,并分析了各参数对其流场速度分布、压力分布和出口轴心速度的影响.仿真值与理论计算值十分接近,数值模拟结果表明,喷嘴收缩角和直径对其内部流场影响较大,而扩张角对其内部流场影响相对较小,但各参数都存在最优值,且各参数之间也存在着最优搭配使射流打击效果最好.     

南京地区中风化较软岩地区嵌岩桩单桩竖向承载力计算分析

对南京地区嵌岩桩桩基设计中新旧国家桩基规范、地方规范及公路和铁路行业规范结合工程实例进行了分析比较,得出了不同规范所得单桩竖向承载力的相应关系,为该地区嵌岩桩设计提供参考.     

宜昌市城区PM_(2.5)污染形成灰霾天气的规律研究

2012年选取与气象站点相邻的一个环境空气质量测点对PM2.5进行了研究性监测,测点距地面23m,全年PM2.5质量浓度在19~284μg/m3之间,年均质量浓度为89μg/m3,月均质量浓度最高的为1月.系统分析全年PM2.5监测质量浓度与相邻气象测点灰霾、能见度观测数据之间的关系,得到以下结论:宜昌市城区PM2.5污染质量浓度与灰霾观测值相关性不强,但与能见度的观测值显著相关;全年能见度降低受PM2.5污染的影响具有季节性,2、5~6三个月与7~9三个月及4、10~11三个月,这3组月份内的PM2.5与能见度之间的回归曲线基本一致,全年中3月份影响最大,而1月份最小,主要与气温、风速、降雨因素有关.