流体神经网络模型在规划物流配送运输方案中的应用

讨论了物流信息系统中物流配送运输方案的规划问题，针对表上作业法、神经网络方法的不足，提出了用流体神经网络来解决这个问题。     

水产养殖业如何面对SPS协定

我国加入WTO后 ,对如何切实发挥政府和水产养殖企业在控制养殖水产品的卫生、安全质量方面的作用 ,充分利用SPS协定有关条款 ,扩大我国养殖水产品的出口 ,有效保护和促进我国民族水产业的可持续健康发展等问题进行了初步的探讨     

CSCW在远程医疗会诊中的应用

讨论了计算机支持的协同工作 (CSCW )的特点和结构 ,结合实际工作需要 ,设计了一个运用CSCW技术进行远程医疗会诊的方案 .分别给出了客户端和服务器端的实现模块 ,采用该方案的专家会诊系统可为患者提供高效、安全的服务     

Prediction of nonregular secondary structures of proteins based on wavelet analysis

The secondary structures of proteins fall into two classes: regular structure and nonregular structure. Helices and sheets are termed "regular" structures because their residues have repeating main-chain torsion angles, and their backbone N-H and C-O groups are arranged in a periodic pattern of hydrogen bonding. In contrast, the remaining structures with nonrepeating backbone torsion angles are called nonregular secondary structures. In this note, we performed an extensive sequence analysis of nonregular secondary structures and showed that these nonregular parts could be effectively predicted by continuous wavelet transform.     

Construction of multivalent DNA vaccines forMycobacterium tuberculosis and its immunogenicity

The coding regions of Ag85B MPT-64, and ESAT-6 secreted proteins were cloned initially into the eukaryotic expression vector pJW4303, then transformed to E. coli Top 10 strain for plasmid DNA extraction and further analysis. Plasmids containing the right insertion were sequenced to confirm their identity. COS7 cells were transfected with a mixture containing serially diluted plasmid DNA encoding three secreted proteins and Lipofectin (Gibco). The supernatants and pellets prepared from various cell lines were run on SDS-PAGE gel and the expression of these proteins in COS7 cells were demonstrated by immunoblot using polyclonal or monoclonal antiserum of M.TBH37Rv. 21 days after first vaccination of C57BL-6 mice by all three recombinant eukaryotic expressing vectors, antibody titer for Ag85B reached 1∶3200. 21 days after second vaccination, the antibody titer reached 1∶102400. The highest antibody levels induced by multivalent vaccines after the second injection were equal to or even greater than the highest antibody levels of single DNA vaccine reported in literature after third injections. Antibody titer of MPT-64 was 1∶50 after the first injection and it reached 1∶200 after the second injection. No antigen-specific antibody against ESAT-6 was detected in sera harvested from immunized mice 21 days after both injections. Antigen-specific IFN-g level of Ag85B was 110 pg/mL while no antigen-specific IFN- g level of ESAT-6 and MPT-64 was detected even after third injections. To our knowledge, it is the first time that studies of polyvalent recombinant DNA vaccines against TB were carried out in C57BL-6 mice. Our results indicated that multiple DNA vaccines could be used to enhance protective responses against M.TB.     

果实成熟与多聚半乳糖醛酸酶基因的研究进展

对与果实成熟相关基因多聚半乳糖醛酸酶 (PG)基因的筛选、鉴定、表达及基因工程的研究进展作一个简要评述     

嗅神经鞘细胞移植恢复大鼠损伤脊髓的功能

为探讨嗅神经鞘细胞移植恢复大鼠损伤脊髓的功能 ,将成年大鼠分为脊髓半切洞损伤组 (A组 )和脊髓半切洞损伤 嗅神经鞘细胞移植组 (B)组 ,手术后应用联合行为评分 (CBS)感觉诱发电位 (SEP)和运动诱发电位 (MEP)检查 ,测定脊髓功能恢复情况 .结果 2组CBS得分 ,A组 >B组 ,SEP和MEP潜峰时均A组 >B组 ,统计学分析均有显著性差异 (P <0 0 5 ) .表明移植嗅神经鞘细胞能恢复损伤脊髓的功能     

应用Elman神经网络的混沌时间序列预测

利用改进的 Elman神经网络对 3个典型的混沌时间序列在不同的噪声水平下进行预测 ,探讨了神经网络学习与泛化之间的关系 ,通过试凑法给出了 Elman最优的隐节点个数。并利用3种指标对预测结果进行了评估 ,结果显示 Elman网络对混沌时间序列预测的良好特性     

衢州珍稀濒危植物区系的研究

调查统计表明,衢州共有野生珍稀濒危植物22种,隶属12科19属,其中国家1级保护植物3种,2级保护植物19种.衢州珍稀濒危植物区系具有种类丰富、过渡性强、成分特有及起源古老等特征.还探讨了它们与邻近地区珍稀植物区系的关系.     

CDMA系统中LMMSE均衡器的时延参数对其性能的影响

介绍了码分多址CDMA系统中码片级线性最小均方误差均衡器。针对时延参数的优化选取对线性最小均方误差均衡器性能的影响进行仿真分析，并同传统rake接收机的性能相比较。结果表明：在均衡器长度与信道最大多径时延接近时，时延参数优化后的线性最小均方误差均衡器与同时延参数未经优化时相比性能有较大改善，而随着均衡器长度的增加，线性最小均方误差均衡器性能对时延参数选取的敏感性逐渐下降。