一种基于驾驶决策的三相交通流仿真模型

提出了一种基于三相交通流理论的驾驶决策交通流仿真模型.该模型采用驾驶决策函数替代由Kerner等人提出的KKW模型中目标速度函数,在新模型中将驾驶行为分为自由流驾驶模式、同步流驾驶模式和堵塞流驾驶模式,三种模式会根据道路的实际交通情况进行切换.基本图和时空分析结果表明,提出的模型符合实际的交通观察.     

中上扬子地区龙马溪组页岩气成藏特征

为解中国南方海相页岩气成藏的基本特征,厘清成藏要素,建立符合中国复杂构造背景下页岩气储层选区评价指标等问题,借鉴美国页岩气储层评价指标,结合目前页岩气勘探评价成果,分析得出:中上扬子地区龙马溪组底部富有机质页岩储层分布连续、埋深适中、构造稳定,富有机质页岩在区域上分布连续,但含气性在局部表现为不连续;根据构造改造的程度,将中上扬子地区龙马溪组页岩分为构造改造区、靠近剥蚀区和构造稳定区三种类型.其中构造稳定区页岩储层表现为超压、含气量高,气井试采初期产量高,是中国中上扬子地区海相页岩气成藏的最有利区.     

掩埋物对声-地震耦合效率的影响

为验证声共振探雷模型对地雷的鉴别能力,研究了不同掩埋物对声．地震耦合效率的影响．首先,简述声波共振技术探测地雷的基本原理及其实验系统;其次,检测在没有埋藏物、埋藏地雷及埋藏砖头3种情况下声波激发的地表振动速度的幅频特性曲线;最后,用有无掩埋物地表振动速度相对值的数据处理方法分析地雷对地表振动的影响．研究结果显示,埋藏地雷与没有埋藏物的地表振动速度的比值在很宽的频带内大于1,地雷能引起地表振动状态的明显变化．表明掩埋地雷能提高声一地震耦合效率,声共振探雷模型具有较强的地雷鉴别能力．     

烟草多酚提取工艺优化及成分定性分析

采用微波辅助技术提取烟草多酚,利用高效液相色谱-光电二级管阵列检测器-质谱联用技术(HPLC-PDA-MS)对烟草多酚主要组分进行定性分析.研究了微波功率、甲醇用量、提取时间和料液比对烟草多酚得率的影响.经响应面优化确定烟草多酚的微波提取条件为:53%甲醇,料液比为1∶11,微波功率为560 W,提取时间为70 s,在此条件下总多酚得率为22.38 mg/g(干重).HPLC-PDA-MS测定表明,烟草多酚的主要组分为绿原酸和芸香苷.     

Characterization of an endo-1,4-β-glucanase from Lilium longiflorum that functioned in pollen germination and pollen tube growth

Endo-β-glucanases play vital roles in the regulation of pollen tube growth. Here, a previously identified endo-l,4-β-glucanase from Lilium Iongiflorum （lily）, named LlpCell, was expressed in Escherichia coli, purified, and further investigated for its physiological function. The recombinant LlpCell protein hydrolyzed carboxy-methylcellulose （CMC） and exhibited activity towards laminarin from Eisenia. arborea and 1,3：l,4-β-glucan of barley. The pH for the optimum activity was 6.0 and the value of Km calculated from CMC was 5.0 mg/mL. Adding EDTA resulted in the total loss of the enzymatic activity, and this effect could be restored by the addition of Ca^2＋. Western blotting analysis showed that LlpCell protein was present in pollen grains and rehydrated pollen grains, and the amount of the protein was increased during pollen germinating, but not in the pollen tube. Consistently, the immunofluorescence labeling study with the antibody against LIpCell also indicated the presence of LIpCell at the begin-ning of germination, but not in the elongating pollen tube. Furthermore, incubation of LlpCell with pollen at the beginning of pollen germination increased the germination percentage and the length of pollen tube. All of these results suggested that LlpCell could play an important role in the regulation of lily pollen germination and the initiation of pollen tube growth.     

基于可用性工程思想的网络地图界面设计—以南京师范大学校园网络地图为例

在对目前网络地图及其界面设计相关研究进行调研与分析的基础上,发现已有的网络地图界面设计缺乏对用户因素的考虑.本文引入可用性工程及设计方法,将其与网络地图界面设计的各个方面相结合,探讨了基于可用性工程思想的网络地图界面设计的过程与方法.以南京师范大学校园网络地图界面设计为例,按照可用性工程的设计思路,设计了针对不同用户功能需求的校园网络地图界面.     

FITC标记的GSTP1蛋白在鼠巨噬细胞中的定位

GSTP1(GSTπ)是人体内谷胱甘肽-S-转移酶(GST)的一种重要的活性亚型,属于机体内源性蛋白,不会引起免疫原反应,在维持细胞的正常机能中发挥着重要作用,也是一种具有临床应用价值的抗氧化剂.本实验室在研究中首先发现GSTP1具有明显的抗炎作用,由此我们设想可以通过增加外源性的GSTP1蛋白来提高其在机体内的水平,以此增强机体的抗炎能力.本实验将薄层色谱扫描和SDS-PAGE相结合用于FITC标记蛋白质的检测,使用激光共聚焦显微镜和流式分析相结合的方法研究了外源GSTP1蛋白在RAW264.7巨噬样细胞和鼠腹腔巨噬细胞中的跨膜转位,结果显示外源GSTP1蛋白能够进入上述细胞.该研究为GSTP1蛋白作为直接使用的药物在抗肿瘤、抗炎、抗氧化等领域的应用提供了实验基础.     

Conformations and adsorption behavior of poly（allylamine hydrochloride） studied by single molecule force spectroscopy

Poly（allylamine hydrochloride） （PAH）, which is frequently used in fabricating polyelectrolyte multilayer films, was studied by single molecule force spectroscopy （SMFS）. Plenty of force-extension curves with a long plateau were obtained in water, indicating that train-like structure was predominant when PAH was adsorbed on the substrate. It was found that the peak-type force-extension curves of PAH in water were not able to be fitted by the modified freely-jointed chain model. Additionally, there was a flat region in the derivative of force-extension curves. Thus, it was inferred that PAH chain in water was in a special conformation and underwent a ＂conformational transition＂ under the stretching of an external force. This phenomenon did not appear in the SMFS experiment in 1 mol/L urea solution, which indicated that urea was able to break the special conformation.     

Verification of GRAPES unified global and regional numerical weather prediction model dynamic core

During the past few years, most of the new developed numerical weather prediction models adopt the strategy of multi-scale technique. Therefore, China Meteorological Administration has devoted to developing a new generation of global and regional multi-scale model since 2003. In order to validate the performance of the GRAPES （Global and Regional Assimilation and PrEdiction System） model both for its scientific design and program coding, a suite of idealized tests has been proposed and conducted, which includes the density flow test, three-dimensional mountain wave and the cross-polar flow test. The density flow experiment indicates that the dynamic core has the ability to simulate the fine scale nonlinear flow structures and its transient features. While the three-dimensional mountain wave test shows that the model can reproduce the horizontal and vertical propagation of internal gravity waves quite well. Cross-polar flow test demonstrates the rationality of both for the semi-Lagrangian departure point calculation and the discretization of the model near the poles. The real case forecasts reveal that the model has the ability to predict the large-scale weather regimes in summer such as the subtropical high, and to capture the major synoptic patterns in the mid and high latitudes.