Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

生产能力扩大的系统动态性研究

利用系统动力学的原理和建模方法描述了企业生产能力与企业物流、信息流与资金流所涉及的各种计划、生产、采购、仓储、销售及财务等职能的关系，利用因果环路图研究分析了生产能力与库存水平、交货期、市场份额、产品价格、成本、利润率、人力等多种因素的因果反馈关系，并构建了生产能力扩大的系统动力学模型来刻画其变化的复杂性与动态性，在两种市场需求（阶跃需求和泊松流随机需求）下模型的仿真结果表明，该模型能较好的反应生产能力扩大的动态行为。     

网络文化对高校学生负面影响的理性思考

随着网络信息技术的迅猛发展,互联网对大学生的影响越来越大,网络文化是一把双刃剑,它具有深远的意义和沉重的“负面效应”。为此,笔者对网络文化的特点及网络文化对大学生的负面影响进行了分析总结,并在此基础上探寻高校教育的新对策,以增强教育的实效性。     

IT系统在企业中的应用

从实际应用的角度，阐述了IT系统在企业中的应用和建设问题。从面向客户的角度出发，对企业IT系统进行了划分，并讨论了各系统之间逻辑关系及其具体功能，在此基础上，对各系统的具体建设内容进行了重点分析。     

浅谈信息时代的电视节目策划

随着信息量的增大，观众对信息的需求出现了多样化和个性化的趋势，传统的电视节目的设置和编排阻碍了电视节目质量的提高，只有顺应时代需要，科学地策划电视节目，才能提高电视的社会效益和经济效益。     

应用化学专业《化工原理》课程教学改革探索

为了适应社会需求的变化和发展，提高学生的工程意识和实践能力，对应用化学专业《化工原理》课程存在的问题与现状进行了分析，探索了该课程教学内容、教学方法、教学手段和实践性教学环节等方面的改革，并在教学实践中予以完善与深化。     

浅谈适龄母猪的饲养管理

针对适龄母猪在妊娠期、分娩期、哺乳期等3个不同的生理阶段的特点。介绍了适龄母猪的饲养管理技术，并对母猪不同症状提出了相应的解决措施。     

PRELIMINARY STUDY ON RETRO-REFLECTIVE COATED PAPER BASED ON MICROGLASS BEADS

INTRODUCTIONThe micro-glass bead[1-2] is a new kind of silicate material, with good properties of chemical stability, mechanical strength as well as electro-resistance. When its diameter is below 0.08mm and the reflective index is in the range of 1.9~2.1, retro-reflective characteristics can be achieved. When a beam of ray from any direction irradiates the surface of the micro-glass beads, it can be converged at the special reflective layer consisted of focuses of micro-glass beads, owing …     

M-泛函的经验似然置信区间

利用经验似然思想，分别讨论不含附加信息和含附加信息时，M-泛函的经验似然置信区间，并推广Zhang Biao (1997) 在独立同分布下的结果。     

罗平布依文化的内涵与价值建构

罗平布依化是我国布依族化的重要组成部分。它既保持着布依整体原生化的优秀内质，又有独特的再生创造话性。其特点是：(1)以美丽的自然环境为裁体；(2)以独特的民俗民趣为形态；(3)以鲜明的民族气质为核心；(4)以丰富的民间艺术形式为见证。强化这些特点，是建构罗平布依化价值的基本思路。“民族尊重”和“宽容姿态”是罗平布依化价值建构的起点和最后归宿。